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Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Accurate forecasting of spatiotemporal data remains challenging due to complex spatial dependencies and temporal dynamics. The inherent uncertainty and variability in such data often render deterministic models insufficient, prompting a…

Machine Learning · Computer Science 2024-11-05 Mingze Gong , Lei Chen , Jia Li

Spatial transcriptomics has revolutionized tissue analysis by simultaneously mapping gene expression, spatial topography, and histological context across consecutive tissue sections, enabling systematic investigation of spatial…

Applications · Statistics 2025-10-24 Meng Zhou , Shuangge Ma , Mengyun Wu

The development of generative models in the past decade has allowed for hyperrealistic data synthesis. While potentially beneficial, this synthetic data generation process has been relatively underexplored in cancer histopathology. One…

Image and Video Processing · Electrical Eng. & Systems 2024-12-10 Aakash Madhav Rao , Debayan Gupta

Spatial transcriptomic (ST) clustering employs spatial and transcription information to group spots spatially coherent and transcriptionally similar together into the same spatial domain. Graph convolution network (GCN) and graph attention…

Quantitative Methods · Quantitative Biology 2023-10-24 Chen Zhang , Junhui Gao , Lingxin Kong , Guangshuo cao , Xiangyu Guo , Wei Liu

Spatial transcriptomics (ST) enables spatially resolved gene profiling but remains expensive and low-throughput, limiting large-cohort studies and routine clinical use. Predicting spatial gene expression from routine hematoxylin and eosin…

Computer Vision and Pattern Recognition · Computer Science 2026-04-24 Changfan Wang , Xinran Wang , Donghai Liu , Fei Su , Lulu Sun , Zhicheng Zhao , Zhu Meng

Recent advancements in spatial transcriptomics technologies allow researchers to simultaneously measure RNA expression levels for hundreds to thousands of genes while preserving spatial information within tissues, providing critical…

Methodology · Statistics 2025-07-31 Catherine Higgins , Jingyi Jessica Li , Michelle Carey

Generative models are used as an alternative data augmentation technique to alleviate the data scarcity problem faced in the medical imaging field. Diffusion models have gathered special attention due to their innovative generation…

Image and Video Processing · Electrical Eng. & Systems 2024-03-27 Ricardo Montoya-del-Angel , Karla Sam-Millan , Joan C Vilanova , Robert Martí

Image synthesis approaches, e.g., generative adversarial networks, have been popular as a form of data augmentation in medical image analysis tasks. It is primarily beneficial to overcome the shortage of publicly accessible data and…

Computer Vision and Pattern Recognition · Computer Science 2023-10-05 Shiyi Du , Xiaosong Wang , Yongyi Lu , Yuyin Zhou , Shaoting Zhang , Alan Yuille , Kang Li , Zongwei Zhou

Score-based Generative Models (SGMs) have demonstrated exceptional synthesis outcomes across various tasks. However, the current design landscape of the forward diffusion process remains largely untapped and often relies on physical…

Machine Learning · Computer Science 2023-10-13 Kushagra Pandey , Stephan Mandt

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

Recent advances in computational pathology have leveraged vision-language models to learn joint representations of Hematoxylin and Eosin (HE) images with spatial transcriptomic (ST) profiles. However, existing approaches typically align HE…

Spatial Transcriptomics (ST) technologies provide biologists with rich insights into single-cell biology by preserving spatial context of cells. Building foundational models for ST can significantly enhance the analysis of vast and complex…

Genomics · Quantitative Biology 2025-07-24 Suyuan Zhao , Yizhen Luo , Ganbo Yang , Yan Zhong , Hao Zhou , Zaiqing Nie

Histopathological diagnoses of tumors in tissue biopsy after Hematoxylin and Eosin (H&E) staining is the gold standard for oncology care. H&E staining is slow and uses dyes, reagents and precious tissue samples that cannot be reused.…

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-12-23 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

Spatial transcriptomics (ST) technologies not only offer an unprecedented opportunity to interrogate intact biological samples in a spatially informed manner, but also set the stage for integration with other imaging-based modalities.…

Quantitative Methods · Quantitative Biology 2025-07-31 Levin M Moser , Ahmad Kamal Hamid , Esteban Miglietta , Nodar Gogoberidze , Beth A Cimini

Spatial transcriptomics provides a molecularly rich description of tissue organization, enabling unsupervised discovery of tissue niches -- spatially coherent regions of distinct cell-type composition and function that are relevant to both…

Computer Vision and Pattern Recognition · Computer Science 2026-04-13 Arbel Hizmi , Artemii Bakulin , Shai Bagon , Nir Yosef

Spatial transcriptomics (ST) has emerged as an advanced technology that provides spatial context to gene expression. Recently, deep learning-based methods have shown the capability to predict gene expression from WSI data using ST data.…

Machine Learning · Computer Science 2024-12-10 Mingcheng Qu , Yuncong Wu , Donglin Di , Anyang Su , Tonghua Su , Yang Song , Lei Fan
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