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In absence of a lens to form an image, incoherent or partially coherent light scattering off an obstructive or reflective object forms a broad intensity distribution in the far field with only feeble spatial features. We show here that…

Accurate assessment of human epidermal growth factor receptor 2 (HER2) expression is critical for breast cancer diagnosis, prognosis, and therapy selection; yet, most existing digital HER2 scoring methods rely on bulky and expensive optical…

Medical Physics · Physics 2026-01-27 Che-Yung Shen , Xilin Yang , Yuzhu Li , Leon Lenk , Aydogan Ozcan

The success of deep learning methods in medical image segmentation tasks heavily depends on a large amount of labeled data to supervise the training. On the other hand, the annotation of biomedical images requires domain knowledge and can…

Computer Vision and Pattern Recognition · Computer Science 2021-09-30 Xinrong Hu , Dewen Zeng , Xiaowei Xu , Yiyu Shi

We present a data-driven approach to compensate for optical aberration in calibration-free quantitative phase imaging (QPI). Unlike existing methods that require additional measurements or a background region to correct aberrations, we…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Taean Chang , Youngju Jo , Gunho Choi , Donghun Ryu , Hyun-Seok Min , Yongkeun Park

The current state of the art in structural biology is led by NMR, X-ray crystallography and TEM investigations. These powerful tools however all rely on averaging over a large ensemble of molecules. Here, we present an alternative concept…

Atomic and Molecular Clusters · Physics 2015-12-15 Tatiana Latychevskaia , Jean-Nicolas Longchamp , Conrad Escher , Hans-Werner Fink

We demonstrate a different scheme to perform optical sectioning of a sample based on the concept of induced coherence [Zou et al., Phys. Rev. Lett. 67, 318 (1991)]. This can be viewed as a different type of optical coherence tomography…

Quantum Physics · Physics 2018-02-19 Adam Vallés , Gerard Jiménez , Luis José Salazar-Serrano , Juan P. Torres

As a label-free imaging technique, quantitative phase imaging (QPI) provides optical path length information of transparent specimens for various applications in biology, materials science, and engineering. Multispectral QPI measures…

Optics · Physics 2023-08-29 Che-Yung Shen , Jingxi Li , Deniz Mengu , Aydogan Ozcan

Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…

Optics · Physics 2025-10-14 Guifeng Li , Chaoyang Gong

Shearing interferometry is a common-path quantitative phase imaging technique in which an object beam interferes with a laterally shifted replica of itself, providing high temporal stability, reduced sensitivity to environmental noise,…

The structural complexity and instability of many interference phase microscopy methods are the major obstacles toward high-precision phase measurement. In this vein, improving more efficient configurations as well as proposing new methods…

Optics · Physics 2020-01-08 Samira Ebrahimi , Masoomeh Dashtdar

Coherent diffractive imaging is a technique that recovers the sample image by numerically inverting its diffraction pattern. We propose a generalization of this method for the inversion of multi-wavelength data. Using this approach, we show…

Optics · Physics 2020-05-08 Erik Malm , Edwin Fohtung , Anders Mikkelsen

This paper presents a novel method for the reconstruction of high-resolution temporal images in dynamic tomographic imaging, particularly for discrete objects with smooth boundaries that vary over time. Addressing the challenge of limited…

Image and Video Processing · Electrical Eng. & Systems 2023-11-10 Ajinkya Kadu , Felix Lucka , Kees Joost Batenburg

Medical image segmentation, which is essential for many clinical applications, has achieved almost human-level performance via data-driven deep learning technologies. Nevertheless, its performance is predicated upon the costly process of…

Computer Vision and Pattern Recognition · Computer Science 2024-04-30 Heejoon Koo

Optical phase-spaces represent fields of any spatial coherence, and are typically measured through phase-retrieval methods involving a computational inversion, interference, or a resolution-limiting lenslet array. Recently, a weak-values…

Optics · Physics 2018-09-12 Samuel H. Knarr , Daniel J. Lum , James Schneeloch , John C. Howell

Non-invasive and single-shot holographic imaging through complex media is technically challenging due to random light scattering which significantly scrambles optical information. Recently, several methods have been presented to address…

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

Quantitative phase imaging (QPI) has been widely applied in characterizing cells and tissues. Spatial light interference microscopy (SLIM) is a highly sensitive QPI method, due to its partially coherent illumination and common path…

Image and Video Processing · Electrical Eng. & Systems 2024-06-12 Yuheng Jiao , Yuchen R. He , Mikhail E. Kandel , Xiaojun Liu , Wenlong Lu , Gabriel Popescu

Quantum entanglement between paired photons is the foundation of optical quantum computing, quantum sensing, and quantum networks. Traditionally, quantum information science has focused on the particle nature of photons at the microscopic…

Quantum Physics · Physics 2024-10-03 Byoung S. Ham

Multiple stochastic signals possess inherent statistical correlations, yet conventional sampling methods that process each channel independently result in data redundancy. To leverage this correlation for efficient sampling, we model…

Signal Processing · Electrical Eng. & Systems 2025-09-18 Lin Jin , Hang Sheng , Hui Feng , Bo Hu

In this work we consider the inverse problem of reconstructing the optical properties of a layered medium from an elastography measurement where optical coherence tomography is used as the imaging method. We hereby model the sample as a…

Numerical Analysis · Mathematics 2024-02-23 Peter Elbau , Leonidas Mindrinos , Leopold Veselka