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Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multi-color capability with high-quality illumination is currently lacking.…

Optics · Physics 2023-03-14 Ning Xu , Sarah E. Bohndiek , Zexing Li , Cilong Zhang , Qiaofeng Tan

We describe a time lens to expand the dynamic range of photon Doppler velocimetry (PDV) systems. The principle and preliminary design of a time-lens PDV (TL-PDV) are explained and shown to be feasible through simulations. In a PDV system,…

Optics · Physics 2021-04-14 Pinghan Chu , Velat Kilic , Mark A. Foster , Zhehui Wang

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

Two-Photon Microscopy (TPM) can provide three-dimensional morphological and functional contrast in vivo. Through proper staining, TPM can be utilized to create virtual, H&E equivalent images and thus can improve throughput in…

Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…

We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light…

Large field-of-view (FOV) microscopic imaging with high lateral resolution (1-2 microns for high space-bandwidth product) plays a pivotal role in biomedicine and biophotonics, especially within the label-free regime, e.g., for whole slide…

This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…

Optics · Physics 2007-05-23 Sergey A. Alexandrov , P. Meredith , T. J. McIntyre , A. V. Zvyagin

Large Language Models (LLMs), known for their versatility in textual data, are increasingly being explored for their potential to enhance medical image segmentation, a crucial task for accurate diagnostic imaging. This study explores…

Image and Video Processing · Electrical Eng. & Systems 2025-08-20 Gurucharan Marthi Krishna Kumar , Aman Chadha , Janine Mendola , Amir Shmuel

Over the last two decades, spatial light modulators (SLMs) have revolutionised our ability to shape optical fields. They grant independent dynamic control over thousands of degrees-of-freedom within a single light beam. In this work we test…

Pretrained Vision Transformers (ViTs) such as DINOv2 and MAE provide generic image features that can be applied to a variety of downstream tasks such as retrieval, classification, and segmentation. However, such representations tend to…

Computer Vision and Pattern Recognition · Computer Science 2026-04-03 Jona Ruthardt , Manu Gaur , Deva Ramanan , Makarand Tapaswi , Yuki M. Asano

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

We present the design and implementation of a side entry laser system designed for an ultra-high vacuum scanning transmission electron microscope. This system uses a versatile probe design enclosed in a vacuum envelope such that parts can…

Numerous applications in quantum and classical optics require scalable, high-speed modulators that cover visible-NIR wavelengths with low footprint, drive voltage (V) and power dissipation. A critical figure of merit for electro-optic (EO)…

Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…

Optics · Physics 2019-04-10 Stephan Amann , Max von Witzleben , and Stefan Breuer

A spatial light modulator (SLM) and a pair of galvanometer-mounted mirrors (GMM) were combined into an optical tweezers set-up. This provides great flexibility as the SLM creates an array of traps which can be moved smoothly and quickly…

Optics · Physics 2009-07-21 Richard D. L. Hanes , Matthew C. Jenkins , Stefan U. Egelhaaf

Exposure to laser light alters cell culture examination via optical microscopic imaging techniques, also based on label-free coherent digital holography. To mitigate this detrimental feature, researchers tend to use a broader spectrum and…

We demonstrate the trapping and manipulation of single neutral atoms in reconfigurable arrays of optical tweezers. Our approach offers unparalleled speed by using a Texas Instruments Digital Micro-mirror Device (DMD) as a holographic…

Atomic Physics · Physics 2018-03-14 Dustin Stuart , Axel Kuhn

The local interaction of charges and light in organic solids is the basis of distinct and fundamental effects. We here observe, at the single molecule scale, how a focused laser beam can locally shift by hundreds-time their natural…

A multi-element interdigitated photoconductive emitter for broadband THz polarization rotation is proposed and experimentally verified. The device consists of separate pixels for the emission of horizontally and vertically polarized THz…

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