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Motivation: In recent years, image-based biological assays have steadily become high-throughput, sparking a need for fast automated methods to extract biologically-meaningful information from hundreds of thousands of images. Taking…

Computer Vision and Pattern Recognition · Computer Science 2021-11-25 Stanley Bryan Z. Hua , Alex X. Lu , Alan M. Moses

Determination of fundamental mechanisms of disease often hinges on histopathology visualization and quantitative image analysis. Currently, the analysis of multi-channel fluorescence tissue images is primarily achieved by manual…

High-content image-based assays have fueled significant discoveries in the life sciences in the past decade (2013-2023), including novel insights into disease etiology, mechanism of action, new therapeutics, and toxicology predictions.…

Subcellular Processes · Quantitative Biology 2024-05-07 Srijit Seal , Maria-Anna Trapotsi , Ola Spjuth , Shantanu Singh , Jordi Carreras-Puigvert , Nigel Greene , Andreas Bender , Anne E. Carpenter

Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an…

Image and Video Processing · Electrical Eng. & Systems 2025-05-06 Shuang Zhang , Carleton Coffin , Karyn L. Rogers , Catherine Ann Royer , Ge Wang

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Live-cell imaging (LCI) technology enables the detailed spatio-temporal characterization of living cells at the single-cell level, which is critical for advancing research in the life sciences, from biomedical applications to bioprocessing.…

Computer Vision and Pattern Recognition · Computer Science 2025-10-09 Johannes Seiffarth , Keitaro Kasahara , Michelle Bund , Benita Lückel , Richard D. Paul , Matthias Pesch , Lennart Witting , Michael Bott , Dietrich Kohlheyer , Katharina Nöh

In this article, we describe the design and implementation of a publicly accessible dermatology image analysis benchmark challenge. The goal of the challenge is to sup- port research and development of algorithms for automated diagnosis of…

Computer Vision and Pattern Recognition · Computer Science 2016-05-05 David Gutman , Noel C. F. Codella , Emre Celebi , Brian Helba , Michael Marchetti , Nabin Mishra , Allan Halpern

Neural cellular automata represent an evolution of the traditional cellular automata model, enhanced by the integration of a deep learning-based transition function. This shift from a manual to a data-driven approach significantly increases…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Andrea Menta , Alberto Archetti , Matteo Matteucci

Automated in-vitro cell detection and counting have been a key theme for artificial and intelligent biological analysis such as biopsy, drug analysis and decease diagnosis. Along with the rapid development of microfluidics and lab-on-chip…

Computer Vision and Pattern Recognition · Computer Science 2020-01-08 Tiancheng Xia , Richard Jiang , YongQing Fu , Nanlin Jin

Cell image classification methods are currently being used in numerous applications in cell biology and medicine. Applications include understanding the effects of genes and drugs in screening experiments, understanding the role and…

Quantitative Methods · Quantitative Biology 2022-03-04 Mohammad Shifat-E-Rabbi , Xuwang Yin , Cailey Elizabeth Fitzgerald , Gustavo K. Rohde

Datasets (semi-)automatically collected from the web can easily scale to millions of entries, but a dataset's usefulness is directly related to how clean and high-quality its examples are. In this paper, we describe and publicly release an…

Computer Vision and Pattern Recognition · Computer Science 2020-08-24 Houda Alberts , Iacer Calixto

An automatic approach to counting any kind of cells could alleviate work of the experts and boost the research in fields such as regenerative medicine. In this paper, a method for microscopy cell counting using multiple frames (hence…

Computer Vision and Pattern Recognition · Computer Science 2018-02-01 Alexander Gomez Villa , Augusto Salazar , Igor Stefanini

Cell state discovery is crucial for understanding biological systems and enhancing medical outcomes. A key aspect of this process is identifying distinct biomarkers that define specific cell states. However, difficulties arise from the…

Human-Computer Interaction · Computer Science 2025-12-19 Rui Sheng , Zelin Zang , Jiachen Wang , Yan Luo , Zixin Chen , Yan Zhou , Shaolun Ruan , Huamin Qu

The current study of cell architecture of inflammation in histopathology images commonly performed for diagnosis and research purposes excludes a lot of information available on the biopsy slide. In autoimmune diseases, major outstanding…

Image and Video Processing · Electrical Eng. & Systems 2022-10-25 Pranav Singh , Jacopo Cirrone

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

We propose a general framework for a collaborative machine learning system to assist bioscience researchers with the task of labeling specific cell identities from microscopic still or video imaging. The distinguishing features of this…

Quantitative Methods · Quantitative Biology 2019-03-25 Greg Bubnis , Steven Ban , Matthew D. DiFranco , Saul Kato

This work summarizes the results of the largest skin image analysis challenge in the world, hosted by the International Skin Imaging Collaboration (ISIC), a global partnership that has organized the world's largest public repository of…

With the growth of artificial intelligence (AI), there has been an increase in the adoption of computer vision and deep learning (DL) techniques for the evaluation of microscopy images and movies. This adoption has not only addressed…

Quantitative Methods · Quantitative Biology 2023-07-21 Binghao Chai , Christoforos Efstathiou , Haoran Yue , Viji M. Draviam

Quantifying cell morphology using images and machine learning has proven to be a powerful tool to study the response of cells to treatments. However, models used to quantify cellular morphology are typically trained with a single microscopy…

Microscopy images from different imaging conditions, organs, and tissues often have numerous cells with various shapes on a range of backgrounds. As a result, designing a deep learning model to count cells in a source domain becomes…

Computer Vision and Pattern Recognition · Computer Science 2023-03-21 Zuhui Wang