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Related papers: Pixel super-resolved virtual staining of label-fre…

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Deep learning-based virtual staining was developed to introduce image contrast to label-free tissue sections, digitally matching the histological staining, which is time-consuming, labor-intensive, and destructive to tissue. Standard…

Image and Video Processing · Electrical Eng. & Systems 2022-10-31 Yijie Zhang , Luzhe Huang , Tairan Liu , Keyi Cheng , Kevin de Haan , Yuzhu Li , Bijie Bai , Aydogan Ozcan

Here, we present a diffusion transformer (DiT)-based pixel super-resolution virtual staining approach to transform low-resolution FTIR microspectroscopic images of the unstained tissues into corresponding high-resolution H&E-stained images.…

Optics · Physics 2026-03-10 Yudong Tian , Xiangyu Zhao , Yuqing Liu , Bofei Yang , Chongzhao Wu

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Histological staining is the gold standard for tissue examination in clinical pathology and life-science research, which visualizes the tissue and cellular structures using chromatic dyes or fluorescence labels to aid the microscopic…

Medical Physics · Physics 2023-03-07 Bijie Bai , Xilin Yang , Yuzhu Li , Yijie Zhang , Nir Pillar , Aydogan Ozcan

Diffusion-model-based image super-resolution techniques often face a trade-off between realistic image generation and computational efficiency. This issue is exacerbated when inference times by decreasing sampling steps, resulting in less…

Computer Vision and Pattern Recognition · Computer Science 2025-07-01 Aradhana Mishra , Bumshik Lee

Visualizing the details of different cellular structures is of great importance to elucidate cellular functions. However, it is challenging to obtain high quality images of different structures directly due to complex cellular environments.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-01 Yi Liu , Hao Yuan , Zhengyang Wang , Shuiwang Ji

Polarization, as a new optical imaging tool, has been explored to assist in the diagnosis of pathology. Moreover, converting the polarimetric Mueller Matrix (MM) to standardized stained images becomes a promising approach to help…

Image and Video Processing · Electrical Eng. & Systems 2025-03-04 Xiaoyu Zheng , Jing Wen , Jiaxin Zhuang , Yao Du , Jing Cong , Limei Guo , Chao He , Lin Luo , Hao Chen

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

Image and Video Processing · Electrical Eng. & Systems 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Virtual staining, or in-silico-labeling, has been proposed to computationally generate synthetic fluorescence images from label-free images by use of deep learning-based image-to-image translation networks. In most reported studies,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-04 Sourya Sengupta , Jianquan Xu , Phuong Nguyen , Frank J. Brooks , Yang Liu , Mark A. Anastasio

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

Image and Video Processing · Electrical Eng. & Systems 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Gram staining has been one of the most frequently used staining protocols in microbiology for over a century, utilized across various fields, including diagnostics, food safety, and environmental monitoring. Its manual procedures make it…

Diffusion probabilistic models learn to remove noise added during training, generating novel data (e.g., images) from Gaussian noise through sequential denoising. However, conditioning the generative process on corrupted or masked images is…

Computer Vision and Pattern Recognition · Computer Science 2026-05-01 Sakshi Agarwal , Gabriel Hope , Jimin Heo , Erik B. Sudderth

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

Staining is essential in cell imaging and medical diagnostics but poses significant challenges, including high cost, time consumption, labor intensity, and irreversible tissue alterations. Recent advances in deep learning have enabled…

Computer Vision and Pattern Recognition · Computer Science 2025-04-15 Ziwang Xu , Lanqing Guo , Satoshi Tsutsui , Shuyan Zhang , Alex C. Kot , Bihan Wen

The advance of diffusion-based generative models in recent years has revolutionized state-of-the-art (SOTA) techniques in a wide variety of image analysis and synthesis tasks, whereas their adaptation on image restoration, particularly…

Image and Video Processing · Electrical Eng. & Systems 2024-07-09 Luzhe Huang , Xiongye Xiao , Shixuan Li , Jiawen Sun , Yi Huang , Aydogan Ozcan , Paul Bogdan

Image-based virtual try-on is an increasingly important task for online shopping. It aims to synthesize images of a specific person wearing a specified garment. Diffusion model-based approaches have recently become popular, as they are…

Computer Vision and Pattern Recognition · Computer Science 2024-04-02 Xu Yang , Changxing Ding , Zhibin Hong , Junhao Huang , Jin Tao , Xiangmin Xu
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