Related papers: Approximations of MINFLUX Localization Precision w…
Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…
Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to nanoscopy. MINFLUX pioneers this transition through point spread function (PSF) engineering, yet its performance is…
MINFLUX (Minimal Photon Flux) is a single-molecule imaging technique capable of resolving fluorophores at a precision of <5 nm. Interpretation of the point patterns generated by this technique presents challenges due to variable emitter…
MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics,…
Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of…
In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…
Localization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the MINFLUX method achieves super-resolution by shaping the excitation point-spread function (PSF) to minimize the…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…
Single nanoparticle tracking using optical microscopy is a powerful technique with many applications in biology, chemistry and material sciences. Despite significant advances, localising objects with nanometric position accuracy in a…
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…
Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterisation of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background…
Super-resolution localization microscopy is based on determining the positions of individual fluorescent markers in a sample. The major challenge in reaching an ever higher localization precision lies in the limited number of collected…
Here, we report analysis and summary of research in the field of localization microscopy for optical imaging. We introduce the basic elements of super-resolved localization microscopy methods for PALM and STORM, commonly used both in vivo…
Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…
An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…
Optical near-fields of small dielectric particles are of particular importance and interests for nanoscale optical engineering such as field localization, fabrication, characterization, sensing and imaging. This paper represents a…
To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…
In this work, we develop several inference methods to estimate the position of dislocations from images generated using dark-field X-ray microscopy (DFXM) -- achieving superresolution accuracy and principled uncertainty quantification.…