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Low image contrast is a major limitation in transmission electron microscopy, since samples with low atomic number only weakly phase-modulate the illuminating electron beam, and beam-induced sample damage limits the usable electron dose.…

Instrumentation and Detectors · Physics 2024-03-19 Jeremy J. Axelrod

Numerous physical phase plates (PP) for phase-contrast enhancement in transmission electron microscopy (TEM) have been proposed and studied with the hole-free or Volta PP having a high impact and interest in recent years. This study is…

Instrumentation and Detectors · Physics 2021-05-25 Simon Hettler , Raul Arenal

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

Quantitative Methods · Quantitative Biology 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

We explore the possibility of applying physical phase plates (PPs) in combination with aberration-corrected transmission electron microscopy. Phase-contrast transfer characteristics are calculated and compared for a thin-film based Zernike…

Instrumentation and Detectors · Physics 2022-05-10 Simon Hettler , Raul Arenal

Phase-plate transmission electron microscopy has recently regressed since a report that Volta phase-plate phase-contrast is less sensitive than non-phase-plate phase contrast, which leads to conventional defocusing phase-contrast. What…

Optics · Physics 2023-07-05 Kuniaki Nagayama

We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser-electron-interaction,…

Instrumentation and Detectors · Physics 2010-09-10 Holger Mueller , Jian Jin , Radostin Danev , John Spence , Howard Padmore , Robert M. Glaeser

We report on a reflective wave-plate system utilizing phase-shifting mirrors (PSM) for a continuous variation of elliptical polarization without changing the beam position and direction. The scalability of multilayer optics to large…

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of…

Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the…

Laser-based preparation, manipulation, and readout of the states of quantum particles has become a powerful research tool that has enabled the most precise measurements of time, fundamental constants, and electromagnetic fields. Laser…

Quantum Physics · Physics 2018-12-12 O. Schwartz , J. J. Axelrod , S. L. Campbell , C. Turnbaugh , R. M. Glaeser , H. Müller

In this theoretical study we analyze contrast transfer of weak-phase objects in a transmission electron microscope, which is equipped with an aberration corrector (Cs-corrector) in the imaging lens system and a physical phase plate in the…

Materials Science · Physics 2010-09-22 B. Gamm , K. Schultheiß , R. R. Schröder , D. Gerthsen

First results on the experimental realisation of a 2x2 programmable phase plate for electrons are presented. The design consists of an array of electrostatic einzel lenses that influence the phase of electron waves passing through 4…

We propose a scheme for constructing a phase plate for use in an ultrafast Zernike-type phase contrast electron microscope, based on the interaction of the electron beam with a strongly focused, high-power femtosecond laser pulse and a…

Instrumentation and Detectors · Physics 2023-03-15 K. A. H. van Leeuwen , W. J. Schaap , B. Buijsse , S. Borelli , S. T. Kempers , W. Verhoeven , O. J. Luiten

In a scanning transmission electron microscope (STEM), producing a high-resolution image generally requires an electron beam focused to the smallest point possible. However, the magnetic lenses used to focus the beam are unavoidably…

Achieving high repeatability and efficiency in laser-induced strong shock wave excitation remains a significant technical challenge, as evidenced by the extensive efforts undertaken at large-scale national laboratories to optimize the…

Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…

Scanning transmission electron microscopy (STEM) provides high-resolution visualization of atomic structures as well as various functional imaging modes utilizing phase contrast. In this study we introduce a semicircular aperture in STEM…

The use of phase plates in the back focal plane of a microscope is a well established technique in optical microscopy to increase the contrast of weakly interacting samples and is gaining interest in electron microscopy as well. In this…

Optics · Physics 2016-08-31 Roeland Juchtmans , Laura Clark , Axel Lubk , Jo Verbeeck

A new generation of microfabricated MEMS for electron optics is changing electron microscopy for the better. These devices allow operations on the electron beam that are impossible with conventional electron optics. Unprecedented phase…

We report initial observations of coherent optical transition radiation interferometry (COTRI) patterns generated by microbunched electrons from laser-driven plasma accelerators (LPAs). These are revealed in the angular distribution…

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