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High-resolution wide field-of-view (FOV) microscopic imaging plays an essential role in various fields of biomedicine, engineering, and physical sciences. As an alternative to conventional lens-based scanning techniques, lensfree holography…

Optics · Physics 2017-06-16 Jialin Zhang , Jiasong Sun , Qian Chen , Jiaji Li , Chao Zuo

Recent advancements in whole slide imaging (WSI) have moved pathology closer to digital practice. Existing systems require precise mechanical control and the cost is prohibitive for most individual pathologists. Here we report a low-cost…

Image and Video Processing · Electrical Eng. & Systems 2019-12-30 Chengfei Guo , Zichao Bian , Shaowei Jiang , Michael Murphy , Jiakai Zhu , Ruihai Wang , Pengming Song , Xiaopeng Shao , Yongbing Zhang , Guoan Zheng

Whole slide imaging (WSI) has recently been cleared for primary diagnosis in the US. A critical challenge of WSI is to perform accurate focusing in high speed. Traditional systems create a focus map prior to scanning. For each focus point…

Computer Vision and Pattern Recognition · Computer Science 2019-04-26 Jun Liao , Yutong Jiang , Zichao Bian , Bahareh Mahrou , Aparna Nambiar , Alexander W. Magsam , Kaikai Guo , Yong Ku Cho , Guoan Zheng

In this paper, scalable Whole Slide Imaging (sWSI), a novel high-throughput, cost-effective and robust whole slide imaging system on both Android and iOS platforms is introduced and analyzed. With sWSI, most mainstream smartphone connected…

Biological Physics · Physics 2017-04-05 Shuoxin Ma , Tan Wang

Whole Slide Imaging (WSI) is a cornerstone of digital pathology, offering detailed insights critical for diagnosis and research. Yet, the gigapixel size of WSIs imposes significant computational challenges, limiting their practical utility.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-15 Ravi Kant Gupta , Shounak Das , Amit Sethi

We report a new lensless microscopy configuration by integrating the concepts of transverse translational ptychography and defocus multi-height phase retrieval. In this approach, we place a tilted image sensor under the specimen for…

Image and Video Processing · Electrical Eng. & Systems 2021-11-03 Shaowei Jiang , Chengfei Guo , Patrick Hu , Derek Hu , Pengming Song , Tianbo Wang , Zichao Bian , Zibang Zhang , Guoan Zheng

Computational imaging methods empower modern microscopy with the ability of producing high-resolution, large field-of-view, aberration-free images. One of the dominant computational label-free imaging methods, Fourier ptychographic…

Optics · Physics 2023-09-06 Ruizhi Cao , Cheng Shen , Changhuei Yang

Background: The use of whole slide imaging (WSI) for digital pathology has recently been cleared for primary diagnosis in the US. A conventional WSI system scans the tissue slide to different positions and acquires the digital images. In a…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Shaowei Jiang , Zichao Bian , Xizhi Huang , Pengming Song , He Zhang , Yongbing Zhang , Guoan Zheng

We present a novel axial ptychographic coherent diffractive imaging (AP-CDI) technique designed to overcome the critical throughput bottleneck of conventional methods. By replacing the 2D raster scan with a simple 1D axial scan, our…

Optics · Physics 2025-11-24 Qijun You , Lingshuo Meng , Fangrui Quan , Wei Cao

The last decade has seen significant advances in computer-aided diagnostics for cytological screening, mainly through the improvement and integration of scanning techniques such as whole slide imaging (WSI) and the combination with deep…

Signal Processing · Electrical Eng. & Systems 2026-01-23 Philip Groult , Julia D. Sistermanns , Ellen Emken , Oliver Hayden , Wolfgang Utschick

Illuminating or imaging samples from a broad angular range is essential in a wide variety of computational 3D imaging and resolution-enhancement techniques, such as optical projection tomography (OPT), optical diffraction tomography (ODT),…

The advancement of digital pathology, particularly through computational analysis of whole slide images (WSI), is poised to significantly enhance diagnostic precision and efficiency. However, the large size and complexity of WSIs make it…

Computer Vision and Pattern Recognition · Computer Science 2024-11-14 Ravi Kant Gupta , Dadi Dharani , Shambhavi Shanker , Amit Sethi

Whole Slide Image (WSI) analysis plays a crucial role in modern digital pathology, enabling large-scale feature extraction from tissue samples. However, traditional feature extraction pipelines based on tools like CellProfiler often involve…

Image and Video Processing · Electrical Eng. & Systems 2025-01-13 Yuechen Yang , Yu Wang , Tianyuan Yao , Ruining Deng , Mengmeng Yin , Shilin Zhao , Haichun Yang , Yuankai Huo

Fourier ptychographic microscopy (FPM), characterized by high-throughput computational imaging, theoretically provides a cunning solution to the trade-off between spatial resolution and field of view (FOV), which has a promising prospect in…

Optics · Physics 2024-02-29 Shuhe Zhang , Aiye Wang , Jinghao Xu , Tianci Feng , Jinhua Zhou , An Pan

Automatic outlining of different tissue types in digitized histological specimen provides a basis for follow-up analyses and can potentially guide subsequent medical decisions. The immense size of whole-slide-images (WSI), however, poses a…

We present a novel diffusion-based approach to generate synthetic histopathological Whole Slide Images (WSIs) at an unprecedented gigapixel scale. Synthetic WSIs have many potential applications: They can augment training datasets to…

Image and Video Processing · Electrical Eng. & Systems 2023-11-15 Robert Harb , Thomas Pock , Heimo Müller

Coherent diffractive imaging (CDI) enables lensless imaging with experimental simplicity and a flexible field of view, yet its resolution is fundamentally constrained by the Abbe diffraction limit. To overcome this limitation, we introduce…

Microscopy imaging systems with a very wide field-of-view (FOV) are highly sought in biomedical applications. In this paper, we report a wide FOV microscopy imaging system that uses a low-cost scanner and a closed-circuit-television (CCTV)…

Optics · Physics 2012-09-19 Guoan Zheng , Xiaoze Ou , Changhuei Yang

The difficulty of processing gigapixel whole slide images (WSIs) in clinical microscopy has been a long-standing barrier to implementing computer aided diagnostic systems. Since modern computing resources are unable to perform computations…

Computer Vision and Pattern Recognition · Computer Science 2020-08-13 Sam Maksoud , Kun Zhao , Peter Hobson , Anthony Jennings , Brian Lovell

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang
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