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Optical microscopy has long been the standard method for diagnosis in cytopathology. Whole slide scanners can image and digitize large sample areas automatically, but are slow, expensive and therefore not widely available. Clinical…

Whole slide images (WSIs) classification represents a fundamental challenge in computational pathology, where multiple instance learning (MIL) has emerged as the dominant paradigm. Current state-of-the-art (SOTA) MIL methods rely on…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Chengying She , Chengwei Chen , Dongjie Fan , Lizhuang Liu , Chengwei Shao , Yun Bian , Ben Wang , Xinran Zhang

We propose and experimentally demonstrate a high-efficiency single-pixel imaging (SPI) scheme by integrating time-correlated single-photon counting (TCSPC) with time-division multiplexing to acquire full-color images at extremely low light…

Histopathology whole slide images (WSIs) are being widely used to develop deep learning-based diagnostic solutions, especially for precision oncology. Most of these diagnostic softwares are vulnerable to biases and impurities in the…

Image and Video Processing · Electrical Eng. & Systems 2024-10-01 Abhijeet Patil , Harsh Diwakar , Jay Sawant , Nikhil Cherian Kurian , Subhash Yadav , Swapnil Rane , Tripti Bameta , Amit Sethi

We present a method for speckle holography that is optimised for crowded fields. Its two key features are an iterativ improvement of the instantaneous Point Spread Functions (PSFs) extracted from each speckle frame and the (optional)…

Instrumentation and Methods for Astrophysics · Physics 2015-05-30 R. Schoedel , S. Yelda , A. Ghez , J. H. V. Girard , L. Labadie , R. Rebolo , A. Perez-Garrido , M. R. Morris

Multi-stain whole-slide-image (WSI) registration is an active field of research. It is unclear, however, how the current WSI registration methods would perform on a real-world data set. AutomatiC Registration Of Breast cAncer Tissue…

Image and Video Processing · Electrical Eng. & Systems 2022-08-29 Satoshi Kondo , Satoshi Kasai , Kousuke Hirasawa

Whole slide image (WSI) classification is a crucial problem for cancer diagnostics in clinics and hospitals. A WSI, acquired at gigapixel size, is commonly tiled into patches and processed by multiple-instance learning (MIL) models.…

Computer Vision and Pattern Recognition · Computer Science 2024-10-10 Doanh C. Bui , Jin Tae Kwak

Label-free imaging approaches seek to simplify and augment histopathologic assessment by replacing the current practice of staining by dyes to visualize tissue morphology with quantitative optical measurements. Quantitative phase imaging…

Whole-slide images (WSIs) from cancer patients contain rich information that can be used for medical diagnosis or to follow treatment progress. To automate their analysis, numerous deep learning methods based on convolutional neural…

Computer Vision and Pattern Recognition · Computer Science 2026-02-18 Lucas Sancéré , Noémie Moreau , Katarzyna Bozek

Second-harmonic generation (SHG) microscopy provides label-free imaging of biological tissues with unique contrast mechanisms, but its resolution is limited by the diffraction limit. Here, we present the first experimental demonstration of…

Optics · Physics 2023-04-28 Jungho Moon , Sungsam Kang , Jin Hee Hong , Seokchan Yoon , Wonshik Choi

Automatic medical image segmentation has wide applications for disease diagnosing. However, it is much more challenging than natural optical image segmentation due to the high-resolution of medical images and the corresponding huge…

Computer Vision and Pattern Recognition · Computer Science 2020-12-18 Ziqiang Li , Rentuo Tao , Qianrun Wu , Bin Li

Plane wave imaging (PWI) with angular compounding has gained in popularity over recent years because it provides high frame rates and good image properties. However, most linear arrays used in clinical practice have a pitch that is equal to…

Signal Processing · Electrical Eng. & Systems 2022-12-08 Zhengchang Kou , Rita J. Miller , Michael L. Oelze

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Whole slide images (WSIs) pose fundamental computational challenges due to their gigapixel resolution and the sparse distribution of informative regions. Existing approaches often treat image patches independently or reshape them in ways…

Computer Vision and Pattern Recognition · Computer Science 2026-05-19 Weiyi Wu , Xingjian Diao , Chunhui Zhang , Chongyang Gao , Xinwen Xu , Siting Li , Jiang Gui

The fast algorithms in Fourier optics have invigorated multifunctional device design and advanced imaging technologies. However, the necessity for fast computations has led to limitations in the widely used conventional Fourier methods,…

Applied Physics · Physics 2024-06-25 Zhi Li , Xuhao Luo , Jing Wang , Xin Yuan , Dongdong Teng , Qiang Song , Huigao Duan

Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity- a common predicament for advancing high-speed and high-throughput cellular imaging. We here…

Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…

Optics · Physics 2025-09-09 Shi Zhao , Haowen Zhou , Changhuei Yang

Immunohistochemical (IHC) stains play a vital role in a pathologist's analysis of medical images, providing crucial diagnostic information for various diseases. Virtual staining from hematoxylin and eosin (H&E)-stained whole slide images…

Image and Video Processing · Electrical Eng. & Systems 2024-10-03 Sitong Liu , Kechun Liu , Samuel Margolis , Wenjun Wu , Stevan R. Knezevich , David E Elder , Megan M. Eguchi , Joann G Elmore , Linda Shapiro

Registration of serial whole-slide histopathological images (WSIs) is critical for enabling direct comparison across diverse stains and for preparing paired datasets in artificial intelligence (AI) workflows such as virtual staining and…

Computer Vision and Pattern Recognition · Computer Science 2025-09-04 Zeyu Liu , Shengwei Ding
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