English
Related papers

Related papers: Fluorescence Diffraction Tomography using Explicit…

200 papers

Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Modern Flash X-ray diffraction Imaging (FXI) acquires diffraction signals from single biomolecules at a high repetition rate from X-ray Free Electron Lasers (XFELs), easily obtaining millions of 2D diffraction patterns from a single…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Jing Liu , Stefan Engblom , Carl Nettelblad

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…

Optics · Physics 2018-01-04 Seungwoo Shin , Doyeon Kim , Kyoohyun Kim , Yongkeun Park

We propose a novel explicit dense 3D reconstruction approach that processes a set of images of a scene with sensor poses and calibrations and estimates a photo-real digital model. One of the key innovations is that the underlying volumetric…

Computer Vision and Pattern Recognition · Computer Science 2022-03-02 Samir Aroudj , Steven Lovegrove , Eddy Ilg , Tanner Schmidt , Michael Goesele , Richard Newcombe

As a critical component of coherent X-ray diffraction imaging (CDI), phase retrieval has been extensively applied in X-ray structural science to recover the 3D morphological information inside measured particles. Despite meeting all the…

Image and Video Processing · Electrical Eng. & Systems 2021-10-29 Longlong Wu , Shinjae Yoo , Ana F. Suzana , Tadesse A. Assefa , Jiecheng Diao , Ross J. Harder , Wonsuk Cha , Ian K. Robinson

Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier…

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

Optics · Physics 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

The non-interference three-dimensional refractive index(RI) tomography has attracted extensive attention in the life science field for its simple system implementation and robust imaging performance. However, the complexity inherent in the…

Optics · Physics 2023-06-13 Delong Yang , Shaohui Zhang , Yao Hu , Qun Hao

Coherent diffraction imaging (CDI) is a promising imaging technique revealing most of the information from diffraction measurements. An ideal CDI should reconstruct complex-valued object from a single-shot far-field diffraction without any…

Optics · Physics 2022-08-26 An-Dong Xiong , Xiao-Peng Jin , Xv-Ri Yao , Qing Zhao

Fluorescence molecular tomography (FMT) is a sensitive optical imaging technology widely used in biomedical research. However, the ill-posedness of the inverse problem poses a huge challenge to FMT reconstruction. Although end-to-end deep…

Image and Video Processing · Electrical Eng. & Systems 2025-03-18 Peng Zhang , Qianqian Xue , Xingyu Liu , Guanglei Zhang , Wenjian Wang , Jiye Liang

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

Image and Video Processing · Electrical Eng. & Systems 2025-04-22 N Goyal , K Khare

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D…

Manufacturers struggle to produce low-cost, robust and complex components at manufacturing lot-size one. Additive processes like Fused Filament Fabrication (FFF) inexpensively produce complex geometries, but defects limit viability in…

Image and Video Processing · Electrical Eng. & Systems 2019-11-14 Joshua E. Siegel , Maria F. Beemer , Steven M. Shepard

Magnetic resonance imaging (MRI) and positron emission tomography (PET) are increasingly used in multimodal analysis of neurodegenerative disorders. While MRI is broadly utilized in clinical settings, PET is less accessible. Many studies…

Image and Video Processing · Electrical Eng. & Systems 2024-11-13 Minhui Yu , Mengqi Wu , Ling Yue , Andrea Bozoki , Mingxia Liu

Acquiring accurate depth information of transparent objects using off-the-shelf RGB-D cameras is a well-known challenge in Computer Vision and Robotics. Depth estimation/completion methods are typically employed and trained on datasets with…

Optical diffraction tomography (ODT) is an emerging 3D imaging technique that is used for the 3D reconstruction of the refractive index (RI) for semi-transparent samples. Various inverse models have been proposed to reconstruct the 3D RI…

Optics · Physics 2022-06-13 Ahmed B. Ayoub , Amirhossein Saba , Carlo Gigli , Demetri Psaltis

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…

Biological Physics · Physics 2017-06-07 Maia Brunstein , Lopamudra Roy , Martin Oheim