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The past decade has seen a revolution in genomic technologies that enable a flood of genome-wide profiling of chromatin marks. Recent literature tried to understand gene regulation by predicting gene expression from large-scale chromatin…

Machine Learning · Computer Science 2017-11-08 Ritambhara Singh , Jack Lanchantin , Arshdeep Sekhon , Yanjun Qi

The spatial organization of complex biochemical reactions is essential for the regulation of cellular processes. Membrane-less structures called foci containing high concentrations of specific proteins have been reported in a variety of…

Amoeboid cell migration is characterized by frequent changes of the direction of motion and resembles a persistent random walk on long time scales. Although it is well known that cell migration is typically driven by the actin cytoskeleton,…

Biological Physics · Physics 2021-06-09 Nicolas Ecker , Karsten Kruse

Focusing on the DNA-bridging nucleoid proteins Fis and H-NS, and integrating several independent experimental and bioinformatic data sources, we investigate the links between chromosomal spatial organization and global transcriptional…

Genomics · Quantitative Biology 2010-12-17 Vittore F. Scolari , Bruno Bassetti , Bianca Sclavi , Marco Cosentino Lagomarsino

Chromatin is a polymer complex of DNA and proteins that regulates gene expression. The three-dimensional structure and organization of chromatin controls DNA transcription and replication. High-throughput chromatin conformation capture…

We investigate the problem of signal transduction via a descriptive analysis of the spatial organization of the complement of proteins exerting a certain function within a cellular compartment. We propose a scheme to assign a numerical…

Molecular Networks · Quantitative Biology 2012-02-03 Federico Felizzi , Jerome Galtier , Georgios Fengos , Dagmar Iber

The classic model of eukaryotic gene expression requires direct spatial contact between a distal enhancer and a proximal promoter. Recent Chromosome Conformation Capture (3C) studies show that enhancers and promoters are embedded in a…

Genomics · Quantitative Biology 2014-11-25 Boryana Doyle , Geoffrey Fudenberg , Maxim Imakaev , Leonid A. Mirny

The genetic instructions stored in the genome require an additional layer of information to robustly determine cell fate. This additional regulation is provided by the interplay between chromosome-patterning biochemical ("epigenetic") marks…

Soft Condensed Matter · Physics 2019-12-04 Davide Coli , Davide Michieletto , Davide Marenduzzo , Enzo Orlandini

A variety of living and non-living systems exhibit collective motion. From swarm robotics to bacterial swarms, and tissue wound healing to human crowds, examples of collective motion are highly diverse but all of them share the common…

Biological Physics · Physics 2023-07-12 Jonathan E. Dawson , Abdul N. Malmi-Kakkada

Transport of molecular motors along protein filaments in a half-closed geometry is a common feature of biologically relevant processes in cellular protrusions. Using a lattice gas model we study how the interplay between active and…

Biological Physics · Physics 2017-03-30 Isabella R. Graf , Erwin Frey

Loop extrusion by motor proteins mediates the attractive interactions in chromatin on the length scale of megabases, providing the polymer with a well-defined structure and at the same time determining its dynamics. The mean square…

Biomolecules · Quantitative Biology 2023-07-12 E. Marchi , Y. Zhan , G. Tiana

By means of a minimal physical model, we investigate the interplay of two phase transitions at play in chromatin organization: (1) liquid-liquid phase separation (LLPS) within the fluid solvating chromatin, resulting in the formation of…

Biological Physics · Physics 2024-09-24 Reda Tiani , Marie Jardat , Vincent Dahirel

A variety of complex mechanisms, from chemical reaction pathways to active fluctuations, orchestrate molecular transport in intracellular environments. Despite significant recent progress in visualizing and probing these processes, little…

Statistical Mechanics · Physics 2024-07-29 Ardra Suchitran , Sreekanth K Manikandan

Keratin are among the most abundant proteins in epithelial cells. Functions of the keratin network in cells are shaped by their dynamical organization. Using a collection of experimentally-driven mathematical models, different hypotheses…

Subcellular Processes · Quantitative Biology 2015-08-19 Stephanie Portet , Anotida Madzvamuse , Andy Chung , Rudolf E. Leube , Reinhard Windoffer

We perform simulations of a system containing simple model proteins and a polymer representing chromatin. We study the interplay between protein-protein and protein-chromatin interactions, and the resulting condensates which arise due to…

Soft Condensed Matter · Physics 2022-07-20 Marco Ancona , Chris A. Brackley

The dispersal of cells from an initially constrained location is a crucial aspect of many physiological phenomena ranging from morphogenesis to tumour spreading. In such processes, the way cell-cell interactions impact the motion of single…

We investigate the large-scale geometry of the DNA-protein complex of chromatin using a generalized optimality principle, which requires that not only should all sub-parts of a natural process be optimal but also the unfolding of higher…

Biomolecules · Quantitative Biology 2024-02-16 Subhash Kak

Active loop extrusion - the process of formation of dynamically growing chromatin loops due to the motor activity of DNA-binding protein complexes - is firmly established mechanism responsible for chromatin spatial organization at different…

Statistical Mechanics · Physics 2023-01-11 Sergey Belan , Vladimir Parfenyev

We use Brownian dynamics simulations to study the formation of chromatin loops through diffusive sliding of slip-link-like proteins, mimicking the behaviour of cohesin molecules. We recently proposed that diffusive sliding is sufficient to…

Soft Condensed Matter · Physics 2020-11-02 Andrea Bonato , Chris A. Brackley , James Johnson , Davide Michieletto , Davide Marenduzzo

A structural relationship between the centrosome and cytoskeleton has been recognized for many years. Centrosomes typically reside near the nucleus, establishing and maintaining the nucleus-centrosome axis. This spatial arrangement is…

Biological Physics · Physics 2024-07-08 Subarna Dutta , Arnab Barua