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Superresolution fluorescence microscopy techniques beat the diffraction limit, enabling ultra-high resolution imaging in biological physics and nanoscience. In all cases that have been studied experimentally, the resolution scales inversely…

Optics · Physics 2012-10-10 Alexander Small

In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…

Optics · Physics 2018-12-13 Oren Solomon , Yonina C. Eldar , Maor Mutzafi , Mordechai Segev

We introduce a practical digital holographic method capable of imaging through a diffusive or scattering medium. The method relies on statistical averaging from a rotating ground glass diffuser to negate the adverse effects caused by…

Spatial Frequency Domain Imaging can map tissue scattering and absorption properties over a wide field of view, making it useful for clinical applications such as wound assessment and surgical guidance. This technique has previously…

Biological Physics · Physics 2021-02-24 Mason T. Chen , Melina Papadakis , Nicholas J. Durr

We report a new coherent imaging technique, termed ptychographic structured modulation (PSM), for quantitative super-resolution microscopy. In this technique, we place a thin diffuser (i.e., a scattering lens) in between the sample and the…

Image and Video Processing · Electrical Eng. & Systems 2019-07-26 Pengming Song , Shaowei Jiang , He Zhang , Zichao Bian , Chengfei Guo , Kazunori Hoshino , Guoan Zheng

Recording and identifying faint objects through atmospheric scattering media by an optical system are fundamentally interesting and technologically important. In this work, we introduce a comprehensive model that incorporates contributions…

Optics · Physics 2024-04-24 Libang Chen , Jun Yang , Lingye Chen , Yuyang Shui , Yikun Liu , Jianying Zhou

The field of biomedical imaging has undergone a rapid growth in recent years, mostly due to the implementation of ad-hoc designed experimental setups, theoretical support methods and numerical reconstructions. Especially for biological…

Biological Physics · Physics 2017-06-30 Daniele Ancora

Image projection through scattering media has applications ranging from light delivery through multimode fiber to near-eye displays. Conventional methods utilize the transmission matrix (TM) measured by interfering with a reference beam.…

When waves propagate through a complex medium, they undergo several scattering events. This phenomenon is detrimental to imaging, as it causes full blurring of the image. Here we describe a method for detecting, localizing and…

Super-resolution algorithms often struggle with images from surveillance environments due to adverse conditions such as unknown degradation, variations in pose, irregular illumination, and occlusions. However, acquiring multiple images,…

Computer Vision and Pattern Recognition · Computer Science 2024-10-22 Marcelo dos Santos , Rayson Laroca , Rafael O. Ribeiro , João C. Neves , David Menotti

A solution to the inversion problem of scattering would offer aberration-free diffraction-limited 3D images without the resolution and depth-of-field limitations of lens-based tomographic systems. Powerful algorithms are increasingly being…

Speckles arise when coherent light interacts with biological tissues. Information retrieval from speckles is desired yet challenging, requiring understanding or mapping of the multiple scattering process, or reliable capability to reverse…

Image and Video Processing · Electrical Eng. & Systems 2020-05-05 Huanhao Li , Zhipeng Yu , Yunqi Luo , Shengfu Cheng , Lihong V. Wang , Yuanjin Zheng , Puxiang Lai

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Manipulating broadband fields in scattering media is a modern challenge across photonics and other wave domains. Recent studies have shown that complex propagation in scattering media can be harnessed to manipulate broadband light wave…

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Deep, high-resolution imaging is essential for unraveling biological complexity and advancing medical diagnostics, yet scattering fundamentally limits optical methods. Among the most promising approaches, photoacoustic imaging achieves…

Adaptive optics can focus light through opaque media by compensating the random phase delay acquired while crossing a scattering curtain. The technique is commonly exploited in many fields, including astrophysics, microscopy, biomedicine…

Optics · Physics 2015-06-24 Diego Di Battista , Giannis Zacharakis , Marco Leonetti

Multiple optical scattering occurs when light propagates in a non-uniform medium. During the multiple scattering, images were distorted and the spatial information they carried became scrambled. However, the image information is not lost…

Computer Vision and Pattern Recognition · Computer Science 2021-07-30 Xinyu Gao , Yi Li , Yanqing Qiu , Bangning Mao , Miaogen Chen , Yanlong Meng , Chunliu Zhao , Juan Kang , Yong Guo , Changyu Shen

Object-motion-based speckle correlation can recover hidden objects from any inhomogeneous medium, which takes advantage of the inherent connection that the cross-correlation between speckle patterns can reflect the autocorrelation of…

Optics · Physics 2023-12-01 Zhao Wang , Rui Ma , Mingzhu She , Anda Shi , Weili Zhang