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Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Light passing through scattering media will be strongly scattered and diffused into complex speckle pattern, which however contains almost all the spatial information and color information of the objects. Although various technologies have…

Image and Video Processing · Electrical Eng. & Systems 2019-07-30 Lei Zhu , Yuxiang Wu , Jietao Liu , Tengfei Wu , Lixian Liu , Xiaopeng Shao

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

Quantum Physics · Physics 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

Imaging with optical resolution through highly scattering media is a long sought-after goal with important applications in deep tissue imaging. Although being the focus of numerous works, this goal was considered impractical until recently.…

Optics · Physics 2012-02-10 Ori Katz , Eran Small , Yaron Silberberg

Scattering, especially multiple scattering, is a well known problem in imaging, ranging from astronomy to medicine. In particular it is often desirable to be able to perform non-invasive imaging through turbid and/or opaque media. Many…

Optics · Physics 2024-04-23 Harry Penketh , Jacopo Bertolotti

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…

Image and Video Processing · Electrical Eng. & Systems 2021-06-07 Figen S. Oktem , Oğuzhan Fatih Kar , Can Deniz Bezek , Farzad Kamalabadi

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

We provide a quantitative analysis of super-resolution imaging techniques which exploit temporal fluctuations of luminosity of the sources in order to beat the Rayleigh limit. We define an operationally justified resolution gain figure of…

Quantum Physics · Physics 2021-06-08 Stanislaw Kurdzialek , Rafal Demkowicz-Dobrzanski

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

We demonstrate focusing and imaging through a scattering medium noninvasively by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning…

Optics · Physics 2019-07-25 Anat Daniel , Dan Oron , Yaron Silberberg

Fluorescence is a powerful mean to probe information processing in the mammalian brain. However, neuronal tissues are highly heterogeneous and thus opaque to light. A wide set of non-invasive or invasive techniques for scattered light…

Optics · Physics 2020-05-11 Claudio Moretti , Sylvain Gigan

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

Optics · Physics 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

Optics · Physics 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

The recent use of diffusion prior, enhanced by pre-trained text-image models, has markedly elevated the performance of image super-resolution (SR). To alleviate the huge computational cost required by pixel-based diffusion SR, latent-based…

Computer Vision and Pattern Recognition · Computer Science 2023-12-14 Feng Luo , Jinxi Xiang , Jun Zhang , Xiao Han , Wei Yang

Flatbed scanners have emerged as promising devices for high-resolution, single-image material capture. However, existing approaches assume very specific conditions, such as uniform diffuse illumination, which are only available in certain…

As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…

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