English
Related papers

Related papers: Advanced analysis of single-molecule spectroscopic…

200 papers

Raman spectroscopy is a non-destructive and label-free chemical analysis technique, which plays a key role in the analysis and discovery cycle of various branches of science. Nonetheless, progress in Raman spectroscopic analysis is still…

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Although Raman spectroscopy is widely used for the investigation of biomedical samples and has a high potential for use in clinical applications, it is not common in clinical routines. One of the factors that obstruct the integration of…

Data Analysis, Statistics and Probability · Physics 2022-01-20 Darina Storozhuk , Oleg Ryabchykov , Juergen Popp , Thomas Bocklitz

Accurate and comprehensive diatomic molecular spectroscopic data have long been vital in a wide variety of applications for measuring and monitoring astrophysical, industrial and other gaseous environments. These data are also used…

Chemical Physics · Physics 2021-03-19 Laura K. McKemmish

Recent advances in snapshot multispectral (MS) imaging have enabled compact, low-cost spectral sensors for consumer and mobile devices. By capturing richer spectral information than conventional RGB sensors, these systems can enhance key…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Luca Cogo , Marco Buzzelli , Simone Bianco , Javier Vazquez-Corral , Raimondo Schettini

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

Functional groups and moieties are chemical descriptors of biomolecules that can be used to interpret their properties and functions, leading to the understanding of chemical or biological mechanisms. These chemical building blocks, or…

Biomolecules · Quantitative Biology 2021-11-08 Yasemin Yesiltepe , Ryan S. Renslow , Thomas O. Metz

Simultaneous multi-slice (SMS) imaging accelerates MRI data acquisition by exciting multiple image slices simultaneously. Overlapping slices are then separated using a mathematical model. Several parameters used in SMS reconstruction impact…

Spectroscopy is the most important method for probing the structure of molecules. However, predicting molecular spectra on classical computers is computationally expensive, with the most accurate methods having a cost that grows…

Quantum Physics · Physics 2025-10-06 Liam P. Flew , Ivan Kassal

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by…

Quantitative Methods · Quantitative Biology 2023-08-09 Afonso Mendes , Hannah S. Heil , Simao Coelho , Christophe Leterrier , Ricardo Henriques

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on…

Quantitative Methods · Quantitative Biology 2015-05-13 Michael C. DeSantis , Shawn H. DeCenzo , Je-Luen Li , Y. M. Wang

This article presents a high-throughput computer program, called EasyDD, for batch processing, analyzing and visualizing of spectral data; particularly those related to the new generation of synchrotron detectors and X-ray powder…

Data Analysis, Statistics and Probability · Physics 2013-07-16 Taha Sochi

Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…

Quantitative Methods · Quantitative Biology 2017-10-24 C. Chen , P. Paul-Gilloteaux , T. Vignaud , J. Salamero , F. Waharte

High-throughput spectrometers are capable of producing data sets containing thousands of spectra for a single biological sample. These data sets contain a substantial amount of redundancy from peptides that may get selected multiple times…

Data Structures and Algorithms · Computer Science 2013-01-08 Fahad Saeed , Trairak Pisitkun , Mark A. Knepper , Jason D. Hoffert

Scanning probe microscopy (SPM) is a valuable technique by which one can investigate the physical characteristics of the surfaces of materials. However, its widespread use is hampered by the time-consuming nature of running an experiment…

Instrumentation and Detectors · Physics 2025-09-03 Nicholas J. Sullivan , Julio J. Valdés , Kirk H. Bevan , Peter Grutter

Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy…

Optics · Physics 2024-10-11 Sanchayeeta Jana , Simon Durst , Markus Lippitz

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

A simplification strategy for Segmented Mirror Splitters (SMS) used as beam combiners is presented. These devices are useful for compact beam division and combination of linear and 2-D arrays. However, the standard design requires unique…

Small Angle Scattering (SAS) of X-rays or neutrons is an experimental technique that provides valuable structural information for biological macromolecules under physiological conditions and with no limitation on the molecular size. In…

Biomolecules · Quantitative Biology 2013-06-11 Konstantin Berlin , Nail A. Gumerov , Ramani Duraiswami , David Fushman