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Complementary DNA strands in solution reliably hybridize to form stable duplexes. We study the kinetics of the hybridization process and the mechanisms by which two initially isolated strands come together to form a stable double helix. We…

Biological Physics · Physics 2019-03-27 Raymond Jin , Lutz Maibaum

The mechanical separation of the double helical DNA structure induced by forces pulling apart the two DNA strands (``unzipping'') has been the subject of recent experiments. Analytical results are obtained within various models of…

Statistical Mechanics · Physics 2009-11-07 D. Marenduzzo , A. Trovato , A. Maritan

We introduce a model of DNA sequence evolution which can account for biases in mutation rates that depend on the identity of the neighboring bases. An analytic solution for this class of non-equilibrium models is developed by adopting…

Biological Physics · Physics 2007-05-23 Peter F. Arndt , Christopher B. Burge , Terence Hwa

DNA cyclization is a powerful technique to gain insight into the nature of DNA bending. The worm-like chain model provides a good description of small to moderate bending fluctuations, but some experiments on strongly-bent shorter molecules…

Biomolecules · Quantitative Biology 2021-12-03 Ryan M. Harrison , Flavio Romano , Thomas E. Ouldridge , Ard A. Louis , Jonathan P. K. Doye

The denaturation of the double helix is a template for fundamental biological functions such as replication and transcription involving the formation of local fluctuational openings. The denaturation transition is studied for heterogeneous…

Biomolecules · Quantitative Biology 2014-06-03 Marco Zoli

We show that a mesoscale model, with a minimal number of parameters, can well describe the thermomechanical and mechanochemical behavior of homogeneous DNA at thermal equilibrium under tension and torque. We predict critical temperatures…

Biomolecules · Quantitative Biology 2015-06-22 Cristiano Nisoli , A. R. Bishop

Using theory and simulations, we carried out a first systematic characterization of DNA unzipping via nanopore translocation. Starting from partially unzipped states, we found three dynamical regimes depending on the applied force, f: (i)…

Soft Condensed Matter · Physics 2023-02-08 Antonio Suma , Vincenzo Carnevale , Cristian Micheletti

In this work we propose a model for DNA double helix within the tight-binding framework that incorporates the helicity of the molecules. We have studied localization properties of three DNAsequences,the periodic poly(dG)-poly(dC) and…

Mesoscale and Nanoscale Physics · Physics 2015-07-27 Sourav Kundu , S. N. Karmakar

Recent advances in operating and manipulating DNA have provided unique experimental possibilities in many fields of DNA research, especially in gene therapy. Researchers have deployed many techniques, experimental and theoretical, to study…

Soft Condensed Matter · Physics 2023-01-04 Arghya Maity , Neha Mathur , Petra Imhof , Navin Singh

Loops are essential secondary structure elements in folded DNA and RNA molecules and proliferate close to the melting transition. Using a theory for nucleic acid secondary structures that accounts for the logarithmic entropy c ln m for a…

Statistical Mechanics · Physics 2011-05-02 Thomas R. Einert , Henri Orland , Roland R. Netz

Here we derive and experimentally test a free energy functional that captures the adhesion of DNA-coated emulsion droplets. Generalizing previous approaches, the theory combines important energetic and entropic effects of microscopic DNA…

Soft Condensed Matter · Physics 2024-06-14 Nicolas Judd , Angus McMullen , Sascha Hilgenfeldt , Jasna Brujic

Specific binding of proteins to DNA is one of the most common ways in which gene expression is controlled. Although general rules for the DNA-protein recognition can be derived, the ambiguous and complex nature of this mechanism precludes a…

Biomolecules · Quantitative Biology 2007-12-17 E. Moroni , M. Caselle , F. Fogolari

DNA has a well-defined structural transition -- the denaturation of its double-stranded form into two single strands -- that strongly affects its thermal transport properties. We show that, according to a widely implemented model for DNA…

Soft Condensed Matter · Physics 2013-02-12 Chih-Chun Chien , Kirill A. Velizhanin , Yonatan Dubi , Michael Zwolak

We investigate diffusion-limited reactions between a diffusing particle and a target site on a semiflexible polymer, a key factor determining the kinetics of DNA-protein binding and polymerization of cytoskeletal filaments. Our theory…

Soft Condensed Matter · Physics 2010-12-08 Yann von Hansen , Roland R. Netz , Michael Hinczewski

An analysis of the stability of a duplex containing G.A mispairs or G.A/A.G tandem during DNA melting has revealed that duplex stability depends on both DNA sequences and on the conformations of the G.A mispairs. The thermodynamics of…

Biological Physics · Physics 2010-08-13 Julia Berashevich , Tapash Chakraborty

It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from its static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with…

Biomolecules · Quantitative Biology 2009-11-13 Nils B. Becker , Ralf Everaers

DNA amplification is the process of replication of a specified DNA sequence \emph{in vitro} through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating…

Biological Physics · Physics 2015-06-23 Karthikeyan Marimuthu , Raj Chakrabarti

Protein-DNA interactions are vital for many processes in living cells, especially transcriptional regulation and DNA modification. To further our understanding of these important processes on the microscopic level, it is necessary that…

Biomolecules · Quantitative Biology 2007-05-23 Jason E Donald , William W Chen , Eugene I Shakhnovich

The present work is devoted to the study of the thermostability of native DNA during binding to TiO$_{2}$ nanoparticles (NPs) at various concentrations under conditions close to physiological (0.1 M Na $^+$, pH 7) using thermal denaturation…

We have measured the temperature driven denaturing, or melting transition in poly d(A)-poly d(T) DNA oligomers of various lengths in different buffer conditions. Our findings are in clear disagreement with two state, reaction kinetics…

Statistical Mechanics · Physics 2016-08-16 G. Zocchi , A. Omerzu , T. Kuriabova , J. Rudnick , G. Grüner