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The spatio-temporal organization of proteins within the cell membrane can affect numerous biological functions, including cell signaling, communication, and transportation. Deviations from normal spatial arrangements have been observed in…

Biomolecules · Quantitative Biology 2023-10-25 Yasaman Moradi , Jerry SH Lee , Andrea M. Armani

Recently, the implementation of plasmonic nanoantennas has opened new possibilities to investigate the nanoscale dynamics of individual biomolecules in living cell. However, studies have yet been restricted to single molecular species as…

The progression of cancer is associated with different genetic and epigenetic events which result in nano to microscale structural alterations in cells/tissue. However, these structural alterations in the early stage of the disease remain…

Coupling between electrical and mechanical phenomena is a near-universal characteristic of inorganic and biological systems alike, with examples ranging from ferroelectric perovskites to electromotor proteins in cellular membranes.…

Materials Science · Physics 2016-11-15 Sergei V. Kalinin , Andrei Rar , Stephen Jesse

The tumor microenvironment (TME) plays a central role in cancer progression, treatment response, and patient outcomes, yet large-scale, consistent, and quantitative TME characterization from routine hematoxylin and eosin (H&E)-stained…

Nanophotonics has become a key enabling technology in biomedicine with great promises in early diagnosis and less invasive therapies. In this context, the unique capability of plasmonic noble metal nanoparticles to concentrate light on the…

Applied Physics · Physics 2017-08-02 Ozlem Yavas , Mikael Svedendahl , Paulina Dobosz , Vanesa Sanz , Romain Quidant

Optical biosensors based on micro-/nano-fibers are highly valuable for probing and monitoring liquid environments and bioactivity. Most of current optical biosensors, however, are still based on glass, semiconductors, or metallic materials,…

Optics · Physics 2025-02-27 X. Yang , L. Xu , S. Xiong , H. Rao , F. Tan , J. Yan , Y. Bao , A. Albanese , A. Camposeo , D. Pisignano , B. Li

All optical nanothermometry has become a powerful, noninvasive tool for measuring nanoscale temperatures in applications ranging from medicine to nanooptics and solid-state nanodevices. The key features of any candidate nanothermometer are…

Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual…

Breast cancer is one of the most challenging global health problems among women. This study investigates the intricate breast tumor microenvironment (TME) dynamics utilizing data from Mammary-specific Polyomavirus Middle T Antigen…

Quantitative Methods · Quantitative Biology 2024-04-11 Navid Mohammad Mirzaei , Panayotis G. Kevrekidis , Leili Shahriyari

Laser illumination of tissue results in a characteristic fluorescence emission spectrum whose features depend on the type of tissue, viz., healthy, adenoma or malignant. Hence measurement of the fluorescence is potentially a rapid and…

There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to…

Biological Physics · Physics 2015-06-23 Mark A. Zurbuchen , Michael P. Lake , Sirus A. Kohan , Belinda Leung , Louis-S. Bouchard

Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier…

Tissues and Organs · Quantitative Biology 2007-05-23 I. C. Baianu , D. Costescu , N. E. Hofmann , S. S. Korban

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

Optics · Physics 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

Scanning probe microscopy is one of the most versatile windows into the nanoworld, providing imaging access to a variety of sample properties, depending on the probe employed. Tunneling probes map electronic properties of samples, magnetic…

Mesoscale and Nanoscale Physics · Physics 2015-01-09 Thomas Häberle , Dominik Schmid-Lorch , Friedemann Reinhard , Jörg Wrachtrup

Nitrogen-vacancy (NV) centers show great potentials for nanoscale bio-sensing and bio-imaging. Nevertheless, their envisioned bio-applications suffer from intrinsic background noise due to unavoidable light scattering and autofluorescence…

Optics · Physics 2024-08-05 Yayin Tan , Xiaolu Wang , Feng Xu , Xinhao Hu , Yuan Lin , Bo Gao , Zhiqin Chu

This paper describes a micro fluorescence in situ hybridization ({\mu}FISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting…

Tissues and Organs · Quantitative Biology 2018-06-19 Deborah Huber , Govind V. Kaigala

Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The…

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Combining optical tweezers with fluorescence microscopy is a powerful tool for single-cell analysis, playing a pivotal role in disease diagnosis, cell sorting, and the investigation of cellular dynamics. However, fluorescence detection…

Optics · Physics 2026-04-07 Jun Yin , Sanyou Chen , Yihao Yan , Mengqi Wang , Ya Wang , Yiheng Lin , Qi Zhang , Fazhan Shi