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Silicon nanochannel field effect transistor (FET) biosensors are one of the most promising technologies in the development of highly sensitive and label-free analyte detection for cancer diagnostics. With their exceptional electrical…

Quantitative Methods · Quantitative Biology 2014-01-07 Pritiraj Mohanty , Yu Chen , Xihua Wang , Mi K. Hong , Carol L. Rosenberg , David T. Weaver , Shyamsunder Erramilli

The development of next generation medicines demand more sensitive and reliable label free sensing able to cope with increasing needs of multiplexing and shorter times to results. Field effect transistor-based biosensors emerge as one of…

Applied Physics · Physics 2019-05-22 Serena Rollo , Dipti Rani , Renaud Leturcq , Wouter Olthuis , César Pascual García

Optical-based nanothermometry represents a transformative approach for precise temperature measurements at the nanoscale, which finds versatile applications across biology, medicine, and electronics. The assembly of ratiometric fluorescent…

Optics · Physics 2025-04-01 Mahshid Iraniparast , Nishant Kumar , Igor Sokolov

Real-time monitoring of dynamic biological processes in the body is critical to understanding disease progression and treatment response. This data, for instance, can help address the lower than 50% response rates to cancer immunotherapy.…

In recent years, light-sheet fluorescence microscopy (LSFM) has emerged as a powerful tool for visualizing and analyzing cancer tissue samples, including patient-derived specimens, organoids, biopsies, and murine models. In this work, we…

Fluorescent nanodiamonds (FNDs) relaxometry holds promising future for advancement of high spatiotemporal resolution metabolic imaging technology. In this study, we demonstrate a simultaneous integration of spatial position tracking with…

Biological Physics · Physics 2025-12-23 Jia Su , Linyu Zeng , Pengyu Chen , Zenghao Kong , Fazhan Shi

Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…

Biological Physics · Physics 2017-11-15 Edakkattuparambil Sidharth Shibu , Nadezda Varkentina , Laurent Cognet , Brahim Lounis

Atomic resolution imaging in transmission electron microscopy (TEM) and scanning TEM (STEM) of light elements in electron-transparent materials has long been a challenge. Biomolecular materials, for example, are rapidly altered when…

Instrumentation and Detectors · Physics 2018-12-05 Fehmi S. Yasin , Tyler R. Harvey , Jordan J. Chess , Jordan S. Pierce , Colin Ophus , Peter Ercius , Benjamin J. McMorran

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

Biosensors are essential tools which have been traditionally used to monitor environmental pollution, detect the presence of toxic elements and biohazardous bacteria or virus in organic matter and biomolecules for clinical diagnostics. In…

Chemical Physics · Physics 2021-01-08 Aishwaryadev Banerjee , Swagata Maity , Carlos H. Mastrangelo

The interactions between tumor cells and the tumor microenvironment (TME) dictate therapeutic efficacy of radiation and many systemic therapies in breast cancer. However, to date, there is not a widely available method to reproducibly…

The living body is composed of innumerable fine and complex structures and although these structures have been studied in the past, a vast amount of information pertaining to them still remains unknown. When attempting to observe these…

Medical Physics · Physics 2021-11-30 Raku Son , Kenji Yamazawa , Akiko Oguchi , Mitsuo Suga , Masaru Tamura , Yasuhiro Murakawa , Satoshi Kume

Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of…

Soft Condensed Matter · Physics 2017-06-21 Luca Puricelli , Massimiliano Galluzzi , Carsten Schulte , Alessandro Podestà , Paolo Milani

The fluorescence of organic fluorophore molecules is enhanced when they are placed in contact with certain metals (Al, Ag, Cu, Au, etc.) whose surface plasmon waves couple into the radiative modes of the molecules and increase the radiative…

Mesoscale and Nanoscale Physics · Physics 2015-04-21 S. Sarkar , B. Kanchibotla , J. D. Nelson , J. D. Edwards , J. Anderson , G. C. Tepper , S. Bandyopadhyay

Despite being extremely old concepts, plasmonics and surface plasmon resonance-based biosensors have been increasingly popular in the recent two decades due to the growing interest in nanooptics and are now of relevant significance in…

Optics · Physics 2023-04-19 Giuseppina Simone

Objective: Survival rate of oral squamous cell carcinoma (OSCC) patients is very poor and can be improved using highly sensitive, specific and accurate techniques. Autofluorescence and fluorescence techniques are very sensitive and useful…

Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

The increasing utilization of charged particle beams for therapeutic purposes requires designing novel detector systems which shall be capable of assessing radiation quality for a diversity of ion species. It is shown that the pattern of…

Instrumentation and Detectors · Physics 2009-09-30 M. Hajek

Viscoelasticity of the cytoplasm plays a critical role in cell morphology and division. In parallel, local temperature is coupled to viscoelasticity and influences cellular bioenergetics. Probing the interdependence of intracellular…

The characterization of nanostructured surfaces with sensitivity in the sub-nm range is of high importance for the development of current and next generation integrated electronic circuits. Modern transistor architectures for e.g. FinFETs…