Related papers: Validation of Golden Gate assemblies using highly …
Golden Gate cloning has become a powerful and widely used DNA assembly method. Its modular nature and the reusability of standardized parts allow rapid construction of transcription units and multi-gene constructs. Importantly, its modular…
Golden Gate cloning has become one of the most popular DNA assembly techniques. Its modular and hierarchical structure allows the construction of complex DNA fragments. Over time, Golden Gate cloning allows for the creation of a repository…
Recombinant protein production is pivotal in molecular biology, enabling profound insights into cellular processes through biophysical, biochemical, and structural analyses of the purified samples. The demand for substantial biomolecule…
Nanopore sequencing technology has the potential to render other sequencing technologies obsolete with its ability to generate long reads and provide portability. However, high error rates of the technology pose a challenge while generating…
Cell-free transcription and translation (TXTL) systems have emerged as a powerful tool for testing genetic regulatory elements and circuits. Cell-free prototyping can dramatically accelerate the design-build-test cycle of new functions in…
The emerging field of DNA storage employs strands of DNA bases (A/T/C/G) as a storage medium for digital information to enable massive density and durability. The DNA storage pipeline includes: (1) encoding the raw data into sequences of…
Oligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning. While currently offering the most affordable source of synthetic DNA, oligo…
We propose that genetic encoding of self-assembling components greatly enhances the evolution of complex systems and provides an efficient platform for inductive generalization, i.e. the inductive derivation of a solution to a problem with…
Expanding genetic codes from natural standard nucleotides to artificial non-standard nucleotides marks a significant advancement in synthetic biology, with profound implications for biotechnology and medicine. Decoding the biological…
We propose an assembly algorithm {\sc Barnacle} for sequences generated by the clone-based approach. We illustrate our approach by assembling the human genome. Our novel method abandons the original physical-mapping-first framework. As we…
The determination of a patient's DNA sequence can, in principle, reveal an increased risk to fall ill with particular diseases [1,2] and help to design "personalized medicine" [3]. Moreover, statistical studies and comparison of genomes [4]…
Most quantum computing architectures to date natively support multi-valued logic, albeit being typically operated in a binary fashion. Multi-valued, or qudit, quantum processors have access to much richer forms of quantum entanglement,…
Programmable assembly of nanoparticles into structures other than hexagonal lattices remains challenging. Assembling an open checkerboard or square lattice is harder to achieve compared to a close-packed hexagonal structure. Here, we…
We construct a sequence of multi-site gates which transform an easily constructed product state into an approximation to the superfluid ground state of the Bose-Hubbard model. The mapping is exact in the one dimensional hard core limit, and…
Geometric quantum computation offers a potential route to fault-tolerant quantum information processing by exploiting the global nature of geometric phases. However, achieving controlled high-order suppression of multiple error sources…
As quantum processors grow in scale and reliability, the need for efficient quantum gate decomposition of circuits to a set of specific available gates, becomes ever more critical. The decomposition of a particular algorithm into a sequence…
We present a detailed description of an architecture for fault-tolerant quantum computation, which is based on the cluster model of encoded qubits. In this cluster-based architecture, concatenated computation is implemented in a quite…
Simple methods to detect biomolecules including specific nucleic acid sequences have received renewed attention since the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus pandemic. Notably, biomolecule detection that uses…
Existing program verifiers can prove advanced properties about security protocol implementations, but are difficult to scale to large codebases because of the manual effort required. We develop a novel methodology called *Diodon* that…
The programmable assembly of DNA strands is a promising tool for building tailored bottom-up nanostructures. Here, we present a plasmonic nanosystem obtained by the base-pairing mediated aggregation of gold nanoparticles (NPs) which are…