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Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…

The Point Spread Function (PSF) is a key figure of merit for specifying the angular resolution of optical systems and, as the demand for higher and higher angular resolution increases, the problem of surface finishing must be taken…

Instrumentation and Methods for Astrophysics · Physics 2016-10-04 K. Tayabaly , D. Spiga , G. Sironi , R. Canestrari , M. Lavagna , G. Pareschi

This Point spread function (PSF) plays a crucial role in many computational imaging applications, such as shape from focus/defocus, depth estimation, and fluorescence microscopy. However, the mathematical model of the defocus process is…

Computer Vision and Pattern Recognition · Computer Science 2022-09-20 Renzhi He , Yan Zhuang , Boya Fu , Fei Liu

Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…

Optics · Physics 2020-07-15 Petar N. Petrov , W. E. Moerner

Simulated images are essential in algorithm development and instrument testing for optical telescopes. During real observations, images obtained by optical telescopes are affected by spatially variable point spread functions (PSFs), a…

Instrumentation and Methods for Astrophysics · Physics 2025-02-17 Zeyu Bai , Peng Jia , Jiameng Lv , Xiang Zhang , Wennan Xiang , Lin Nie

Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but their performance depends critically on the distribution and number of functional groups on their surface. When studying…

Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity…

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…

Image and Video Processing · Electrical Eng. & Systems 2022-08-05 Bhargav Ghanekar , Vishwanath Saragadam , Dushyant Mehra , Anna-Karin Gustavsson , Aswin Sankaranarayanan , Ashok Veeraraghavan

The key features of the MATPHOT algorithm for precise and accurate stellar photometry and astrometry using discrete Point Spread Functions are described. A discrete Point Spread Function (PSF) is a sampled version of a continuous PSF which…

Astrophysics · Physics 2009-11-11 Kenneth J. Mighell

The point spread function (PSF) reflects states of a telescope and plays an important role in development of data processing methods, such as PSF based astrometry, photometry and image restoration. However, for wide field small aperture…

Instrumentation and Methods for Astrophysics · Physics 2021-07-07 Peng Jia , Xuebo Wu , Zhengyang Li , Bo Li , Weihua Wang , Qiang Liu , Adam Popowicz

Accurate astrometry and photometry of saturated and coronagraphic point spread functions (PSFs) are fundamental to both ground- and space-based high contrast imaging projects. For ground-based adaptive optics imaging, differential…

Astrophysics · Physics 2008-11-26 C. Marois , D. Lafreniere , B. Macintosh , R. Doyon

In single molecule orientation localization microscopy, valuable information about the orientation and longitudinal position of each molecule is often encoded in the shape of the point spread function (PSF). This shape, though, can be…

Precise knowledge of the point spread function (PSF) underpins many data analysis steps in astronomy, from photometry and astrometry to source de-blending and deconvolution. In adaptive optics (AO) observations, however, the PSF is highly…

Instrumentation and Methods for Astrophysics · Physics 2026-04-02 Arseniy Kuznetsov , Benoit Neichel , Sylvain Oberti , Thierry Fusco

Point Spread Function (PSF) modeling is a central part of any astronomy data analysis relying on measuring the shapes of objects. It is especially crucial for weak gravitational lensing, in order to beat down systematics and allow one to…

Instrumentation and Methods for Astrophysics · Physics 2015-05-30 Joel Bergé , Sedona Price , Adam Amara , Jason Rhodes

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

The past decade has brought many innovations in optical design for 3D super-resolution imaging of point-like emitters, but these methods often focus on single-emitter localization precision as a performance metric. Here, we propose a simple…

Optics · Physics 2022-09-28 James M. Jusuf , Matthew D. Lew

Accurate modelling of the effective point spread function (ePSF) is essential for high-precision photometry and astrometry, particularly in undersampled imaging regimes. In this work, we build on a well-established ePSF modelling framework…

Instrumentation and Methods for Astrophysics · Physics 2025-12-19 Emma Godden , Katherine M. Blundell

Point spread function (PSF) reconstruction (PSF-R) is a well established technique to determine reliably and accurately the PSF from Adaptive Optics (AO) control loop data. We have successfully applied this technique to improve the…

Instrumentation and Methods for Astrophysics · Physics 2020-04-15 Olivier Beltramo-Martin , Antonino Marasco , Thierry Fusco , Davide Massari , Julien Milli , Giuliana Fiorentino , B. Neichel

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman
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