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Important applications of single-particle tracking (SPT) aim at deciphering the diffusion properties of single fluorescent nanoparticles immersed in heterogeneous environments, such as multi-cellular biological tissues. To maximize the…

Access to knowledge of the point spread function (PSF) of adaptive optics(AO)-assisted observations is still a major limitation when processing AO data. This limitation is particularly important when image analysis requires the use of…

Instrumentation and Methods for Astrophysics · Physics 2023-05-10 A. Lau , R. JL. Fétick , B. Neichel , O. Beltramo-Martin , T. Fusco

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

Scanning Kelvin probe microscopy (SKPM) is a powerful technique for investigating the electrostatic properties of material surfaces, enabling the imaging of variations in work function, topology, surface charge density, or combinations…

Applied Physics · Physics 2024-07-26 Isaac C. D. Lenton , Felix Pertl , Lubuna Shafeek , Scott R. Waitukaitis

In astronomy, upcoming space telescopes with wide-field optical instruments have a spatially varying point spread function (PSF). Specific scientific goals require a high-fidelity estimation of the PSF at target positions where no direct…

Instrumentation and Methods for Astrophysics · Physics 2023-02-22 Tobias Liaudat , Jean-Luc Starck , Martin Kilbinger , Pierre-Antoine Frugier

Point-spread-function (PSF) engineering is a well-established computational imaging technique that uses phase masks and other optical elements to embed extra information (e.g., depth) into the images captured by conventional CMOS image…

Computer Vision and Pattern Recognition · Computer Science 2024-06-14 Sachin Shah , Matthew Albert Chan , Haoming Cai , Jingxi Chen , Sakshum Kulshrestha , Chahat Deep Singh , Yiannis Aloimonos , Christopher Metzler

The accurate modelling of the Point Spread Function (PSF) is of paramount importance in astronomical observations, as it allows for the correction of distortions and blurring caused by the telescope and atmosphere. PSF modelling is crucial…

Instrumentation and Methods for Astrophysics · Physics 2023-09-25 Tobias Liaudat , Jean-Luc Starck , Martin Kilbinger

Point spread function (PSF) engineering has been pivotal in the remarkable progress made in high-resolution imaging in the last decades. However, the diversity in PSF structures attainable through existing engineering methods is limited.…

Optics · Physics 2025-06-10 Md Sadman Sakib Rahman , Aydogan Ozcan

Point spread function (PSF) engineering is vital for precisely controlling the focus of light in computational imaging, with applications in neural imaging, fluorescence microscopy, and biophotonics. The PSF is derived from the magnitude of…

Optics · Physics 2025-04-22 Aleksey Valouev

We consider the high-resolution imaging problem of 3D point source image recovery from 2D data using a method based on point spread function (PSF) engineering. The method involves a new technique, recently proposed by S.~Prasad, based on…

Signal Processing · Electrical Eng. & Systems 2019-06-13 Chao Wang , Raymond Chan , Mila Nikolova , Robert Plemmons , Sudhakar Prasad

Point spread function (PSF) plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view as astigmatism, coma and vignetting. Thus, one should…

Computer Vision and Pattern Recognition · Computer Science 2017-08-01 Anna Jezierska , Hugues Talbot , Jean-Christophe Pesquet , Gilbert Engler

We describe a rapid and direct method for regularizing, post-facto, the point-spread function (PSF) of a telescope or other imaging instrument, across its entire field of view. Imaging instruments in general blur point sources of light by…

Instrumentation and Methods for Astrophysics · Physics 2023-03-20 J. M. Hughes , C. E. DeForest , D. B. Seaton

Planned wide-field weak lensing surveys are expected to reduce the statistical errors on the shear field to unprecedented levels. In contrast, systematic errors like those induced by the convolution with the point spread function (PSF) will…

Cosmology and Nongalactic Astrophysics · Physics 2012-10-23 M. Gentile , F. Courbin , G. Meylan

Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…

Optics · Physics 2024-07-11 Hanzhe Wu , Danni Chen , YiHong Jiand Gan Xiang , Heng Li , Bin Yu , JunLe Qu

Modern Giant Segmented Mirror Telescopes (GSMTs) like the Extremely Large Telescope, which is currently under construction, depend heavily on Adaptive Optics (AO) systems to correct for atmospheric distortions. However, a residual blur…

Instrumentation and Methods for Astrophysics · Physics 2023-07-05 Roland Wagner , Jenny Niebsch , Ronny Ramlau

We present the development of a data-driven, AI-based model of the Point Spread Function (PSF) that achieves higher accuracy than the current state-of-the-art approach, "PSF in the Full Field-of-View'' (PIFF). PIFF is widely used in leading…

Instrumentation and Methods for Astrophysics · Physics 2026-02-18 Dayana Andrea Henao Arbeláez , Pierre-François Léget , Andrés Alejandro Plazas Malagón

Modeling the Point Spread Function (PSF) of wide-field surveys is vital for many astrophysical applications and cosmological probes including weak gravitational lensing. The PSF smears the image of any recorded object and therefore needs to…

Instrumentation and Methods for Astrophysics · Physics 2018-07-26 Jörg Herbel , Tomasz Kacprzak , Adam Amara , Alexandre Refregier , Aurelien Lucchi

Incoherently illuminated or luminescent objects give rise to a low-contrast speckle-like pattern when observed through a thin diffusive medium, as such a medium effectively convolves their shape with a speckle-like point spread function…

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

Point-wise localization of individual fluorophores is a critical step in super-resolution microscopy and single particle tracking. Although the methods are limited by the accuracy in localizing individual flourophores, this point-wise…

Biological Physics · Physics 2017-05-08 Martin Lindén , Vladimir Ćurić , Elias Amselem , Johan Elf