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In fluorescence microscopy, spectral unmixing aims to recover individual fluorophore concentrations from spectral images that capture mixed fluorophore emissions. Since classical methods operate pixel-wise and rely on least-squares fitting,…

Computer Vision and Pattern Recognition · Computer Science 2026-03-26 Federico Carrara , Talley Lambert , Mehdi Seifi , Florian Jug

Optical imaging deep inside scattering media remains a fundamental problem in bio-imaging. While wavefront shaping has been shown to allow focusing of coherent light at depth, achieving it non-invasively remains a challenge. Various…

Optics · Physics 2019-12-24 Antoine Boniface , Baptiste Blochet , Jonathan Dong , Sylvain Gigan

Two-photon (2P) microscopy is a powerful technique for deep-tissue fluorescence imaging; however, tissue scattering limits its effectiveness for depth imaging using conventional approaches. Despite typical strategies having been put forward…

Optics · Physics 2025-05-21 Lei Zhu , Bernhard Rauer , Hilton B. De Aguiar , Sylvain Gigan

Memory-effect-based methods have been demonstrated to be feasible to observe hidden objects through thin scattering layers, even from a single-shot speckle pattern. However, most of the existing methods are performed with narrowband…

Image and Video Processing · Electrical Eng. & Systems 2018-09-20 Tengfei Wu , Chengfei Guo , Xiaopeng Shao

High-contrast imaging for the detection and characterization of exoplanets relies on the instrument's capability to block out the light of the host star. Some current post-processing methods for calibrating out the residual speckles use…

Instrumentation and Methods for Astrophysics · Physics 2015-06-15 Marie Ygouf , Laurent M. Mugnier , David Mouillet , Thierry Fusco , Jean-Luc Beuzit

Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…

Optics · Physics 2026-03-04 Qi Hu , Jingyu Wang , Huriye Atilgan , Armin Lak , Martin J. Booth

MRI reconstruction techniques based on deep learning have led to unprecedented reconstruction quality especially in highly accelerated settings. However, deep learning techniques are also known to fail unexpectedly and hallucinate…

Image and Video Processing · Electrical Eng. & Systems 2023-08-08 Paul Fischer , Thomas Küstner , Christian F. Baumgartner

Robust and accurate camera calibration is essential for 3D reconstruction in light microscopy under circular motion. Conventional methods require either accurate key point matching or precise segmentation of the axial-view images. Both…

Computer Vision and Pattern Recognition · Computer Science 2019-10-31 Yuanhao Guo , Fons J. Verbeek , Ge Yang

Optical imaging systems are inherently imperfect due to diffraction limits, lens manufacturing tolerances, assembly misalignment, and other physical constraints. In addition, unavoidable camera shake and object motion further introduce…

Computer Vision and Pattern Recognition · Computer Science 2025-11-17 Yanlong Yang , Guanxiong Luo

Motivated by their great potential to reduce the size, cost and weight, flat lenses, a category that includes diffractive lenses and metalenses, are rapidly emerging as key components with the potential to replace the traditional refractive…

Optics · Physics 2023-08-25 Roy Maman , Eitan Mualem , Noa Mazurski , Jacob Engelberg , Uriel Levy

Lensless fiber endomicroscope is an emerging tool for in-vivo microscopic imaging, where quantitative phase imaging (QPI) can be utilized as a label-free method to enhance image contrast. However, existing single-shot phase reconstruction…

Ground-based solar image restoration is a computationally expensive procedure that involves nonlinear optimization techniques. The presence of atmospheric turbulence produces perturbations in individual images that make it necessary to…

Instrumentation and Methods for Astrophysics · Physics 2023-07-26 A. Asensio Ramos , S. Esteban Pozuelo , C. Kuckein

Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data. While deconvolution methods and other…

Computer Vision and Pattern Recognition · Computer Science 2017-04-06 Martin Weigert , Loic Royer , Florian Jug , Gene Myers

Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

This paper demonstrates a practical method that can correct spatial varying blur from a set of images of the same object. The algorithm jointly estimates the object and local point spread functions~(PSF). The method prioritizes sections…

Image and Video Processing · Electrical Eng. & Systems 2020-11-04 Wouter van de Ketterij , Oleg Soloviev , Michel Verhaegen

Motion blur estimation remains an important task for scene analysis and image restoration. In recent years, the removal of motion blur in photographs has seen impressive progress in the hands of deep learning-based methods, trained to map…

Computer Vision and Pattern Recognition · Computer Science 2021-04-28 Guillermo Carbajal , Patricia Vitoria , Mauricio Delbracio , Pablo Musé , José Lezama

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Structured light, where complex optical fields are tailored in all their degrees of freedom, has become highly topical of late, advanced by a sophisticated toolkit comprising both linear and nonlinear optics. Removing undesired structure…

Traditional feature-based image stitching technologies rely heavily on feature detection quality, often failing to stitch images with few features or low resolution. The learning-based image stitching solutions are rarely studied due to the…

Computer Vision and Pattern Recognition · Computer Science 2021-07-07 Lang Nie , Chunyu Lin , Kang Liao , Shuaicheng Liu , Yao Zhao

Frequency upconversion technique offers an appealing approach for sensitive mid-infrared (MIR) imaging at room temperature. However, the spatial resolution of the upconversion imager has been notoriously restricted by the limited transverse…