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In single particle coherent x-ray diffraction imaging experiments, performed at x-ray free-electron lasers (XFELs), samples are exposed to intense x-ray pulses to obtain single-shot diffraction patterns. The high intensity induces…

Atomic and Molecular Clusters · Physics 2013-01-29 U. Lorenz , N. M. Kabachnik , E. Weckert , I. A. Vartanyants

Single-pixel imaging (SPI) is a novel imaging technique whose working principle is based on the compressive sensing (CS) theory. In SPI, data is obtained through a series of compressive measurements and the corresponding image is…

Image and Video Processing · Electrical Eng. & Systems 2022-07-15 Stephen L. H. Lau , Edwin K. P. Chong

Single biomolecular imaging using XFEL radiation is an emerging method for protein structure determination using the "diffraction before destruction" method at near atomic resolution. Crucial parameters for such bio-imaging experiments are…

Single-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification. However, the…

Biomolecules · Quantitative Biology 2024-11-26 Tong You , Johan Bielecki , Filipe R. N. C. Maia

Single-shot diffraction imaging of isolated nanosized particles has seen remarkable success in recent years, yielding in-situ measurements with ultra-high spatial and temporal resolution. The progress of high-repetition-rate sources for…

Atomic and Molecular Clusters · Physics 2022-08-26 Julian Zimmermann , Fabien Beguet , Daniel Guthruf , Bruno Langbehn , Daniela Rupp

The short and intense pulses of the new X-ray free electron lasers, now operational or under construction, may make possible diffraction experiments on single molecule-sized objects with high resolution, before radiation damage destroys the…

Data Analysis, Statistics and Probability · Physics 2012-04-11 Miklós Tegze , Gábor Bortel

With X-ray free-electron lasers (XFELs), it is possible to determine the three-dimensional structure of noncrystalline nanoscale particles using X-ray single-particle imaging (SPI) techniques at room temperature. Classifying SPI scattering…

Machine Learning · Computer Science 2023-02-15 Cong Wang , Eric Florin , Hsing-Yin Chang , Jana Thayer , Chun Hong Yoon

Modern x-ray free-electron lasers (XFELs) produce x-ray pulses of exceptional transverse coherence. This is due largely to the process of optical guiding by which the radiation is both refractively guided by the bunched electron beam and…

Accelerator Physics · Physics 2021-04-20 River R. Robles , Gabriel Marcus , Zhirong Huang

Single particle cryo-electron microscopy is a vital tool for 3D characterization of protein structures. A typical workflow involves acquiring projection images of a collection of randomly oriented particles, picking and classifying…

Image and Video Processing · Electrical Eng. & Systems 2021-03-22 S. V. Venkatakrishnan , Puneet Juneja , Hugh O'Neill

Recovery of three-dimensional structure from single particle X-ray scattering of completely randomly oriented diffraction patterns as predicted few decades back has been real due to the advent of the new emerging X-ray Free Electron Laser…

Soft Condensed Matter · Physics 2015-11-24 M. Uddin

Speden is a computer program that reconstructs the electron density of single particles from their x-ray diffraction patterns, using a single-particle adaptation of the Holographic Method in crystallography. (Szoke, A., Szoke, H., and…

X-ray Free Electron Lasers (XFEL) are revolutionary photons sources, whose ultrashort, brilliant pulses are expected to allow single molecule diffraction experiments providing structural information on the atomic length scale. This ultimate…

Optics · Physics 2015-05-20 Andrea Fratalocchi , Giancarlo Ruocco

By circumventing the resolution limitations of optics, coherent diffractive imaging (CDI) and ptychography are making their way into scientific fields ranging from X-ray imaging to astronomy. Yet, the need for time consuming iterative phase…

Computational Physics · Physics 2023-10-13 Oliver Hoidn , Aashwin Ananda Mishra , Apurva Mehta

Single particle imaging (SPI) is a promising method for native structure determination which has undergone a fast progress with the development of X-ray Free-Electron Lasers. Large amounts of data are collected during SPI experiments,…

Image and Video Processing · Electrical Eng. & Systems 2021-04-23 Alexandr Ignatenko , Dameli Assalauova , Sergey A. Bobkov , Luca Gelisio , Anton B. Teslyuk , Viacheslav A. Ilyin , Ivan A. Vartanyants

Single-shot X-ray imaging of short-lived nanostructures such as clusters and nanoparticles near a phase transition or non-crystalizing objects such as large proteins and viruses is currently the most elegant method for characterizing their…

Atomic and Molecular Clusters · Physics 2020-10-14 Thomas Stielow , Robin Schmidt , Christian Peltz , Thomas Fennel , Stefan Scheel

Understanding complex biological macromolecules, especially proteins, is vital for grasping their diverse chemical functions with direct impact in biology and pharmacology. While techniques like X-ray crystallography and cryo-electron…

Biomolecules · Quantitative Biology 2024-04-12 S. H. Mejias , A. L. Cortajarena , R. Mincigrucci , C. Svetina , C. Masciovecchio

Machine learning is attracting surging interest across nearly all scientific areas by enabling the analysis of large datasets and the extraction of scientific information from incomplete data. Data-driven science is rapidly growing,…

Applied Physics · Physics 2025-03-17 Sung Yun Lee , Do Hyung Cho , Chulho Jung , Daeho Sung , Daewoong Nam , Sangsoo Kim , Changyong Song

An improved analysis for single particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the AMO instrument at the Linac Coherent Light Source (LCLS) as…

Proposals to determine biomolecular structures from diffraction experiments using femtosecond X-ray free-electron laser (XFEL) pulses involve a conflict between the incident brightness required to achieve diffraction-limited atomic…

Optics · Physics 2015-05-19 Harry M. Quiney , Keith A. Nugent

We reconstructed intensities in Fourier space and electron densities in the real space for an azimuthally symmetric object Nanorice particle (Iron Oxide nanoparticle) exposed in the ultrashort, bright and coherent x-ray free electron laser…

Biological Physics · Physics 2020-07-14 S. S. Kim , P. Nepal , D. K. Saldin , C. H. Yoon