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Cryo-electron tomography (Cryo-ET) is a powerful tool in structural biology for 3D visualization of cells and biological systems at resolutions sufficient to identify individual proteins in situ. The measurements are collected by tilting…

Signal Processing · Electrical Eng. & Systems 2025-09-03 Vinith Kishore , Valentin Debarnot , AmirEhsan Khorashadizadeh , Ivan Dokmanić

Deep neural networks for medical image reconstruction are traditionally trained using high-quality ground-truth images as training targets. Recent work on Noise2Noise (N2N) has shown the potential of using multiple noisy measurements of the…

Image and Video Processing · Electrical Eng. & Systems 2021-12-21 Weijie Gan , Yu Sun , Cihat Eldeniz , Jiaming Liu , Hongyu An , Ulugbek S. Kamilov

Cryo-electron tomography (cryo-ET) enables 3D visualization of cellular structures. Accurate reconstruction of high-resolution volumes is complicated by the very low signal-to-noise ratio and a restricted range of sample tilts. Recent…

Image and Video Processing · Electrical Eng. & Systems 2026-03-03 Vinith Kishore , Valentin Debarnot , AmirEhsan Khorashadizadeh , Ricardo D. Righetto , Benjamin D. Engel , Ivan Dokmanić

We present a Machine Learning-based method for tomographic reconstruction of dense layered objects, with range of projection angles limited to $\pm $10$^\circ$. Whereas previous approaches to phase tomography generally require two steps,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-08 Alexandre Goy , Girish Rughoobur , Shuai Li , Kwabena Arthur , Akintunde I. Akinwande , George Barbastathis

Cryo-electron tomography (cryoET) is a technique that captures images of biological samples at different tilts, preserving their native state as much as possible. Along with the partial tilt series and noise, one of the major challenges in…

Signal Processing · Electrical Eng. & Systems 2023-07-26 Vinith Kishore , Valentin Debarnot , Ivan Dokmanić

Electron tomography is becoming an increasingly important tool in materials science for studying the three-dimensional morphologies and chemical compositions of nanostructures. The image quality obtained by many current algorithms is…

A long-standing challenge in tomography is the 'missing wedge' problem, which arises when the acquisition of projection images within a certain angular range is restricted due to geometrical constraints. This incomplete dataset results in…

Materials Science · Physics 2025-03-26 Chonghang Zhao , Mingyuan Ge , Xiaogang Yang , Yong S. Chu , Hanfei Yan

Microscopy image analysis often requires the segmentation of objects, but training data for this task is typically scarce and hard to obtain. Here we propose DenoiSeg, a new method that can be trained end-to-end on only a few annotated…

Computer Vision and Pattern Recognition · Computer Science 2020-06-12 Tim-Oliver Buchholz , Mangal Prakash , Alexander Krull , Florian Jug

Electron tomography is a powerful tool for understanding the morphology of materials in three dimensions, but conventional reconstruction algorithms typically suffer from missing-wedge artifacts and data misalignment imposed by experimental…

Image and Video Processing · Electrical Eng. & Systems 2025-12-10 Cedric Lim , Corneel Casert , Arthur R. C. McCray , Serin Lee , Andrew Barnum , Jennifer Dionne , Colin Ophus

Limited-angle electron tomography aims to reconstruct 3D shapes from 2D projections of Transmission Electron Microscopy (TEM) within a restricted range and number of tilting angles, but it suffers from the missing-wedge problem that causes…

Computer Vision and Pattern Recognition · Computer Science 2026-05-05 Zhantao Deng , Mériem Er-Rafik , Anna Sushko , Cécile Hébert , Pascal Fua

Electron tomographic reconstruction is a method for obtaining a three-dimensional image of a specimen with a series of two dimensional microscope images taken from different viewing angles. Filtered backprojection, one of the most popular…

Computer Vision and Pattern Recognition · Computer Science 2018-08-01 Chen Mu , Chiwoo Park

Ultrafast electron beam X-ray computed tomography produces noisy data due to short measurement times, causing reconstruction artifacts and limiting overall image quality. To counteract these issues, two self-supervised deep learning methods…

Machine Learning · Computer Science 2025-11-24 Israt Jahan Tulin , Sebastian Starke , Dominic Windisch , André Bieberle , Peter Steinbach

In recent years, deep learning-based image compression, particularly through generative models, has emerged as a pivotal area of research. Despite significant advancements, challenges such as diminished sharpness and quality in…

Image and Video Processing · Electrical Eng. & Systems 2024-09-18 Ryugo Morita , Hitoshi Nishimura , Ko Watanabe , Andreas Dengel , Jinjia Zhou

Most of the classical denoising methods restore clear results by selecting and averaging pixels in the noisy input. Instead of relying on hand-crafted selecting and averaging strategies, we propose to explicitly learn this process with deep…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Xiangyu Xu , Muchen Li , Wenxiu Sun

In this study we explore the possibility to use deep learning for the reconstruction of phase images from 4D scanning transmission electron microscopy (4D-STEM) data. The process can be divided into two main steps. First, the complex…

Materials Science · Physics 2023-02-15 Thomas Friedrich , Chu-Ping Yu , Jo Verbeeck , Sandra Van Aert

Electrical impedance tomography is an imaging modality for extracting information on the conductivity distribution inside a physical body from boundary measurements of current and voltage. In many practical applications, it is a priori…

Numerical Analysis · Mathematics 2014-06-06 Lauri Harhanen , Nuutti Hyvönen , Helle Majander , Stratos Staboulis

We propose a framework to jointly determine the deformation parameters and reconstruct the unknown volume in electron cryotomography (CryoET). CryoET aims to reconstruct three-dimensional biological samples from two-dimensional projections.…

Image and Video Processing · Electrical Eng. & Systems 2023-10-17 Valentin Debarnot , Sidharth Gupta , Konik Kothari , Ivan Dokmanic

Cryo-electron microscopy (cryo-EM) has achieved near-atomic level resolution of biomolecules by reconstructing 2D micrographs. However, the resolution and accuracy of the reconstructed particles are significantly reduced due to the…

Computer Vision and Pattern Recognition · Computer Science 2024-01-03 Jing Zhang , Tengfei Zhao , ShiYu Hu , Xin Zhao

Recovering a high-quality image from noisy indirect measurements is an important problem with many applications. For such inverse problems, supervised deep convolutional neural network (CNN)-based denoising methods have shown strong…

Image and Video Processing · Electrical Eng. & Systems 2020-09-16 Allard A. Hendriksen , Daniel M. Pelt , K. Joost Batenburg

Electron tomography, as an important 3D imaging method, offers a powerful method to probe the 3D structure of materials from the nano- to the atomic-scale. However, as a grant challenge, radiation intolerance of the nanoscale samples and…

Materials Science · Physics 2020-03-30 Chunyang Wang , Guanglei Ding , Yitong Liu , Huolin L. Xin
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