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Digital PCR (dPCR) has revolutionized nucleic acid diagnostics by enabling absolute quantification of rare mutations and target sequences. However, current detection methodologies face challenges, as flow cytometers are costly and complex,…

Quantitative Methods · Quantitative Biology 2024-03-29 Yuanyuan Wei , Shanhang Luo , Changran Xu , Yingqi Fu , Qingyue Dong , Yi Zhang , Fuyang Qu , Guangyao Cheng , Yi-Ping Ho , Ho-Pui Ho , Wu Yuan

Accurate molecular quantification is essential for advancing research and diagnostics in fields such as infectious diseases, cancer biology, and genetic disorders. Droplet digital PCR (ddPCR) has emerged as a gold standard for achieving…

Quantitative Methods · Quantitative Biology 2025-01-17 Yuanyuan Wei , Yucheng Wu , Fuyang Qu , Yao Mu , Yi-Ping Ho , Ho-Pui Ho , Wu Yuan , Mingkun Xu

Real-time PCR, or Real-time Quantitative PCR (qPCR) is an effective approach to quantify nucleic acid samples. Given the complicated reaction system along with thermal cycles, there has been long-term confusion on accurately calculating the…

Quantitative Methods · Quantitative Biology 2021-03-02 Chuanbo Liu , Jin Wang

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

The increasing use of microfluidics in industrial, biomedical, and clinical applications requires a more and more precise control of the microfluidic flows and suspended particles or cells. This leads to higher demands in three-dimensional…

Fluid Dynamics · Physics 2020-11-10 Massimiliano Rossi , Rune Barnkob

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Flow-Imaging Microscopy (FIM) is commonly used in both academia and industry to characterize subvisible particles (those $\le 25 \mu m$ in size) in protein therapeutics. Pharmaceutical companies are required to record vast volumes of FIM…

Quantitative Methods · Quantitative Biology 2017-09-04 Christopher P. Calderon , Austin L. Daniels , Theodore W. Randolph

Recent advancements in machine learning achieved by Deep Neural Networks (DNNs) have been significant. While demonstrating high accuracy, DNNs are associated with a huge number of parameters and computations, which leads to high memory…

Machine Learning · Computer Science 2023-12-20 Babak Rokh , Ali Azarpeyvand , Alireza Khanteymoori

Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available. In the area of measurement technology,…

Quantitative Methods · Quantitative Biology 2019-08-20 Yueqin Li , Ata Mahjoubfar , Claire Lifan Chen , Kayvan Reza Niazi , Li Pei , Bahram Jalali

Automated in-vitro cell detection and counting have been a key theme for artificial and intelligent biological analysis such as biopsy, drug analysis and decease diagnosis. Along with the rapid development of microfluidics and lab-on-chip…

Computer Vision and Pattern Recognition · Computer Science 2020-01-08 Tiancheng Xia , Richard Jiang , YongQing Fu , Nanlin Jin

Motivation: Protein embedding, which represents proteins as numerical vectors, is a crucial step in various learning-based protein annotation/classification problems, including gene ontology prediction, protein-protein interaction…

Genomics · Quantitative Biology 2024-05-21 Jiayu Shang , Cheng Peng , Yongxin Ji , Jiaojiao Guan , Dehan Cai , Xubo Tang , Yanni Sun

We describe a novel experimental setup that combines the advantages of both laser-induced fluorescence and cavity ring-down techniques. The simultaneous and correlated measurement of the ring-down and fluorescence signals yields absolute…

Chemical Physics · Physics 2013-10-15 Scott E. Sanders , Oliver R. Willis , N. Hendrik Nahler , Eckart Wrede

The deep neural network is a research hotspot for histopathological image analysis, which can improve the efficiency and accuracy of diagnosis for pathologists or be used for disease screening. The whole slide pathological image can reach…

Image and Video Processing · Electrical Eng. & Systems 2022-05-09 Tingting Zheng , Weixing chen , Shuqin Li , Hao Quan , Qun Bai , Tianhang Nan , Song Zheng , Xinghua Gao , Yue Zhao , Xiaoyu Cui

Sub-visible particle analysis using flow imaging microscopy combined with deep learning has proven effective in identifying particle types, enabling the distinction of harmless components such as silicone oil from protein particles.…

Computer Vision and Pattern Recognition · Computer Science 2025-08-11 Utku Ozbulak , Michaela Cohrs , Hristo L. Svilenov , Joris Vankerschaver , Wesley De Neve

Deep neural network (DNN) quantization converting floating-point (FP) data in the network to integers (INT) is an effective way to shrink the model size for memory saving and simplify the operations for compute acceleration. Recently,…

Machine Learning · Computer Science 2020-01-01 Yukuan Yang , Shuang Wu , Lei Deng , Tianyi Yan , Yuan Xie , Guoqi Li

Accurately modeling the protein fitness landscapes holds great importance for protein engineering. Recently, due to their capacity and representation ability, pre-trained protein language models have achieved state-of-the-art performance in…

Biomolecules · Quantitative Biology 2024-02-06 Ziyi Zhou , Liang Zhang , Yuanxi Yu , Mingchen Li , Liang Hong , Pan Tan

Diffusion MRI (dMRI) is the primary imaging modality used to study brain microstructure in vivo. Reliable and computationally efficient parameter inference for common dMRI biophysical models is a challenging inverse problem, due to factors…

Image and Video Processing · Electrical Eng. & Systems 2025-03-03 William Consagra , Lipeng Ning , Yogesh Rathi

Protein function is inherently linked to its localization within the cell, and fluorescent microscopy data is an indispensable resource for learning representations of proteins. Despite major developments in molecular representation…

Quantitative Methods · Quantitative Biology 2022-05-25 Anastasia Razdaibiedina , Alexander Brechalov

Traditional deep learning-based methods for classifying cellular features in microscopy images require time- and labor-intensive processes for training models. Among the current limitations are major time commitments from domain experts for…

Image and Video Processing · Electrical Eng. & Systems 2025-01-22 Abhiram Kandiyana , Peter R. Mouton , Yaroslav Kolinko , Lawrence O. Hall , Dmitry Goldgof

Machine- and deep-learning approaches for biological sequences depend critically on transforming raw DNA, RNA, and protein FASTA files into informative numerical representations. However, this process is often fragmented across multiple…

Genomics · Quantitative Biology 2025-12-01 Hamid Ismail , Marwan Bikdash
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