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Exposure to intense illumination light is an unavoidable consequence of fluorescence microscopy, and poses a risk to the health of the sample in every live-cell fluorescence microscopy experiment. Furthermore, the possible side-effects of…

Computer Vision and Pattern Recognition · Computer Science 2017-01-24 David Richmond , Anna Payne-Tobin Jost , Talley Lambert , Jennifer Waters , Hunter Elliott

The complexity of human biology and its intricate systems holds immense potential for advancing human health, disease treatment, and scientific discovery. However, traditional manual methods for studying biological interactions are often…

Other Quantitative Biology · Quantitative Biology 2025-01-24 Himanshu Buckchash , Gyanendra Kumar Verma , Dilip K. Prasad

Artificial intelligence (AI) is being deployed within radiology at a rapid pace. AI has proven an excellent tool for reconstructing and enhancing images that appear sharper, smoother, and more detailed, can be acquired more quickly, and…

Artificial Intelligence · Computer Science 2026-02-11 Jana G. Delfino , Jason L. Granstedt , Frank W. Samuelson , Robert Ochs , Krishna Juluru

4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…

Image and Video Processing · Electrical Eng. & Systems 2026-02-17 Cunmin Zhao , Ziyuan Luo , Guoye Guan , Zelin Li , Yiming Ma , Zhongying Zhao , Renjie Wan

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

The brightfield microscope is instrumental in the visual examination of both biological and physical samples at sub-millimeter scales. One key clinical application has been in cancer histopathology, where the microscopic assessment of the…

Computer Vision and Pattern Recognition · Computer Science 2020-06-03 Po-Hsuan Cameron Chen , Krishna Gadepalli , Robert MacDonald , Yun Liu , Kunal Nagpal , Timo Kohlberger , Jeffrey Dean , Greg S. Corrado , Jason D. Hipp , Martin C. Stumpe

Research in photonic computing has flourished due to the proliferation of optoelectronic components on photonic integration platforms. Photonic integrated circuits have enabled ultrafast artificial neural networks, providing a framework for…

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at sub-micron…

Optics · Physics 2021-04-08 Yi Zhang , Haonan Zong , Cheng Zong , Yuying Tan , Meng Zhang , Yuewei Zhan , Ji-Xin Cheng

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy,…

Quantitative Methods · Quantitative Biology 2024-01-04 Hari Shroff , Ilaria Testa , Florian Jug , Suliana Manley

With the growth of artificial intelligence (AI), there has been an increase in the adoption of computer vision and deep learning (DL) techniques for the evaluation of microscopy images and movies. This adoption has not only addressed…

Quantitative Methods · Quantitative Biology 2023-07-21 Binghao Chai , Christoforos Efstathiou , Haoran Yue , Viji M. Draviam

Artificial intelligence represents a new frontier in human medicine that could save more lives and reduce the costs, thereby increasing accessibility. As a consequence, the rate of advancement of AI in cancer medical imaging and more…

Recently, there has been great interest in developing Artificial Intelligence (AI) enabled computer-aided diagnostics solutions for the diagnosis of skin cancer. With the increasing incidence of skin cancers, low awareness among a growing…

Image and Video Processing · Electrical Eng. & Systems 2020-06-23 Manu Goyal , Thomas Knackstedt , Shaofeng Yan , Saeed Hassanpour

Optical microscopy is an indispensable tool in life sciences research, but conventional techniques require compromises between imaging parameters like speed, resolution, field-of-view, and phototoxicity. To overcome these limitations,…

Quantitative Methods · Quantitative Biology 2024-04-01 Leonor Morgado , Estibaliz Gómez-de-Mariscal , Hannah S. Heil , Ricardo Henriques

Real-time computer-aided diagnosis using artificial intelligence (AI), with images, can help oncologists diagnose cancer with high accuracy and in an early phase. We reviewed real-time AI-based analyzed images for decision-making in…

Image and Video Processing · Electrical Eng. & Systems 2025-03-17 Leila Bagheriye , Johan Kwisthout

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

Cancer is one of the leading causes of death worldwide. Fast and safe early-stage, pre- and intra-operative diagnostics can significantly contribute to successful cancer identification and treatment. Artificial intelligence has played an…

Tissues and Organs · Quantitative Biology 2022-08-10 Marina Zajnulina

Diabetes mellitus (DM) predisposes patients to vascular complications. Retinal images and vasculature reflect the body's micro- and macrovascular health. They can be used to diagnose DM complications, including diabetic retinopathy (DR),…

Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation,…

Instrumentation and Detectors · Physics 2025-09-16 Ian Coghill , Alienor Lahlou , Andrea Lodetti , Shizue Matsubara , Johann Boucle , Thomas Le Saux , Ludovic Jullien

Artificial Intelligence (AI) technology is based on theory and development of computer systems able to perform tasks that normally require human intelligence. In this context, deep learning is a family of computational methods that allow an…

Computers and Society · Computer Science 2019-05-17 Mario Coccia

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard
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