Related papers: Optical Diffraction Tomography Meets Fluorescence …
Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…
Confocal laser scanning microscopy (CLSM) stands out as one of the most widely used microscopy techniques, thanks to its three-dimensional imaging capability and its sub-diffraction spatial resolution, achieved through the closure of a…
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…
Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…
Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…
Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be…
Image deblurring aims to restore the detailed texture information or structures from blurry images, which has become an indispensable step in many computer vision tasks. Although various methods have been proposed to deal with the image…
Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…
Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…
Light-field microscopy (LFM) is a 3D microscopy technique whereby volumetric information of a sample is gained by simultaneously capturing both the position and momentum (angular) information of light illuminating a scene. Conventional LFM…
Fluorescence molecular tomography (FMT) is an emerging powerful tool for biomedical research. There are two factors that influence FMT reconstruction most effectively. The first one is the regularization techniques. Traditional methods such…
With the invention of scanning probe techniques, direct imaging of single atoms and molecules became possible. Today, scanning tunnelling microscopy (STM) routinely provides angstrom-scale image resolution. At the same time, however, STM…
Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…
Schlieren deflectometry aims at characterizing the deflections undergone by refracted incident light rays at any surface point of a transparent object. For smooth surfaces, each surface location is actually associated with a sparse…
Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit…
Shape from Polarization (SfP) estimates surface normals using photos captured at different polarizer rotations. Fundamentally, the SfP model assumes that light is reflected either diffusely or specularly. However, this model is not valid…
The modulation transfer function (MTF) is widely used to characterise the performance of optical systems. Measuring it is costly and it is thus rarely available for a given lens specimen. Instead, MTFs based on simulations or, at best, MTFs…
We consider a set of M images, whose pixel intensities at a common point can be treated as the components of a M-dimensional vector. We are interested in the estimation of the modulus of such a vector associated to a compact source. For…
We propose and demonstrate a new imaging technique to noninvasively see through scattering layers with the aid of a spatial light modulator (SLM). A relay system projects the incoherent light pattern emitting from the scattering layer onto…