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Atomic resolution imaging in transmission electron microscopy (TEM) and scanning TEM (STEM) of light elements in electron-transparent materials has long been a challenge. Biomolecular materials, for example, are rapidly altered when…

Instrumentation and Detectors · Physics 2018-12-05 Fehmi S. Yasin , Tyler R. Harvey , Jordan J. Chess , Jordan S. Pierce , Colin Ophus , Peter Ercius , Benjamin J. McMorran

The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…

Optics · Physics 2022-09-07 Lukas Wesemann , Jon Rickett , Timothy J. Davis , Ann Roberts

The phase-resolved imaging of confined light fields by homodyne detection is a cornerstone of metrology in nano-optics and photonics, but its application in electron microscopy has been limited so far. Here, we report the mapping of optical…

Mesoscale and Nanoscale Physics · Physics 2024-08-27 Jonathan T. Weber , Niklas Müller , Alexander Schröder , Sascha Schäfer

Recent progress in phase modulation using nanofabricated electron holograms has demonstrated how the phase of an electron beam can be controlled. In this paper, we apply this concept to the correction of spherical aberration in a scanning…

Despite the tremendous progresses in wavefront control through or inside complex scattering media, several limitations prevent reaching practical feasibility for nonlinear imaging in biological tissues. While the optimization of nonlinear…

Optics · Physics 2016-12-06 Hilton B. de Aguiar , Sylvain Gigan , Sophie Brasselet

Contrast transfer mechanisms for electron scattering have been extensively studied in transmission electron microscopy. Here we revisit H. Rose's generalized contrast formalism from scattering theory to understand where information is…

Optics · Physics 2025-10-28 Desheng Ma , Guanxing Li , David A Muller , Steven E Zeltmann

Phase imaging techniques extract the optical path-length information of a scene, whereas wavefront sensors provide the shape of an optical wavefront. Since these two applications have different technical requirements, they have developed…

Optics · Physics 2018-02-28 F. Soldevila , V. Durán , P. Clemente , J. Lancis , E. Tajahuerce

We employ ptychography, a phase-retrieval imaging technique, to show experimentally for the first time that a partially coherent high-energy matter (electron) wave emanating from an extended source can be decomposed into a set of mutually…

Mesoscale and Nanoscale Physics · Physics 2017-01-04 S. Cao , P. Kok , P. Li , A. M. Maiden , J. M. Rodenburg

Characterization and control of the transverse phase space of high-brightness electron beams is required at free-electron lasers or electron diffraction experiments for emittance measurement and beam optimization as well as at advanced…

Scanning transmission electron microscopy is a common tool used to study the atomic structure of materials. It is an inherently multimodal tool allowing for the simultaneous acquisition of multiple information channels. Despite its…

Forescatter electron imaging is a popular microscopy technique, especially for scanning electron microscopes equipped with an electron backscatter diffraction detector. In principal, this method enables qualitative imaging of microstructure…

Materials Science · Physics 2018-06-04 Ben Britton , Daniel Goran , Vivian Tong

Tracing the motion of electrons has enormous relevance to understanding ubiquitous phenomena in ultrafast science, such as the dynamical evolution of the electron density during complex chemical and biological processes. Scattering of…

Atomic Physics · Physics 2013-03-28 Gopal Dixit , Jan Malte Slowik , Robin Santra

Coherent Diffractive Imaging is a lensless technique that allows imaging of matter at a spatial resolution not limited by lens aberrations. This technique exploits the measured diffraction pattern of a coherent beam scattered by periodic…

Computational Physics · Physics 2025-08-27 Alessandro Colombo , Davide Emilio Galli , Liberato De Caro , Francesco Scattarella , Elvio Carlino

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Electron ptychography provides highly sensitive, dose efficient phase images which can be corrected for aberrations after the data has been acquired. This is crucial when very precise quantification is required, such as with sensitivity to…

Materials Science · Physics 2023-07-27 Christoph Hofer , Timothy J. Pennycook

Here a new microscopic method is proposed to image and characterize very thin samples like few-layer materials, organic molecules, and nanostructures with nanometer or sub-nanometer resolution using electron beams of energies lower than 20…

Instrumentation and Detectors · Physics 2016-01-06 Ing-Shouh Hwang

This letter proposes a new method based on ultrafast high energy electron radiography to diagnose transient electromagnetic field. For the traditional methods, large scattering from matter will increase the uncertainty of measurement, but…

Accelerator Physics · Physics 2022-02-09 J. H. Xiao , Y. C. Du , H. Q. Li , Y. T. Zhao , L. Sheng

Phase imaging is gaining importance due to its applications in fields like biomedical imaging and material characterization. In biomedical applications, it can provide quantitative information missing in label-free microscopy modalities.…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Gabriel della Maggiora , Luis Alberto Croquevielle , Harry Horsley , Thomas Heinis , Artur Yakimovich

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

Quantitative Methods · Quantitative Biology 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

Not long after the laser was invented, it has been marked as a candidate source of strong, high-frequency electromagnetic radiation for acceleration of particles. Indeed, while the complex particle accelerator facilities today are an…

Accelerator Physics · Physics 2022-05-25 Roy Shiloh , Tomas Chlouba , Peter Hommelhoff