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Lensless flexible fiber-bundle based endoscopes allow imaging at depths beyond the reach of conventional microscopes with a minimal footprint. These multicore fibers provide a simple solution for widefield fluorescent imaging when the…

Optics · Physics 2020-08-26 Noam Shekel , Ori Katz

We propose and experimentally demonstrate a differential microscopy method to obtain simultaneous amplitude, phase, and quantitative polarization gradient imaging in a single experimental embodiment. A full-field optical spatial…

Optics · Physics 2023-10-11 Jeeban Kumar Nayak , Niladri Modak , Sayan Ghosh , Nirmalya Ghosh

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

Multiplex Imaging (MI) enables the simultaneous visualization of multiple biological markers in separate imaging channels at subcellular resolution, providing valuable insights into cell-type heterogeneity and spatial organization. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-11-07 Simon Gutwein , Daria Lazic , Thomas Walter , Sabine Taschner-Mandl , Roxane Licandro

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Quantum entanglement and squeezing have significantly improved phase estimation and imaging in interferometric settings beyond the classical limits. However, for a wide class of non-interferometric phase imaging/retrieval methods vastly…

This work presents the Griffith-type phase-field formation at large deformation in the framework of adaptive edge-based smoothed finite element method (ES-FEM) for the first time. Therein the phase-field modeling of fractures has attracted…

Numerical Analysis · Mathematics 2021-11-09 Fucheng Tian , Xiaoliang Tang , Tingyu Xu , Liangbin Li

The complex physics behind electroadhesion-based tactile displays poses an enormous modeling challenge since not only the fingerpad structure with multiple nonlinear layers, but also the roughness at the microscopic scale play a decisive…

Soft Condensed Matter · Physics 2022-12-19 Fabian Forsbach , Markus Heß , Antonio Papangelo

Raster-scan optoacoustic mesoscopy (RSOM) is a powerful, non-invasive optical imaging technique for functional, anatomical, and molecular skin and tissue analysis. However, both the manual and the automated analysis of such images are…

Image and Video Processing · Electrical Eng. & Systems 2020-12-04 Stefan Gerl , Johannes C. Paetzold , Hailong He , Ivan Ezhov , Suprosanna Shit , Florian Kofler , Amirhossein Bayat , Giles Tetteh , Vasilis Ntziachristos , Bjoern Menze

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…

Skin diseases affect millions of people worldwide, across all ethnicities. Increasing diagnosis accessibility requires fair and accurate segmentation and classification of dermatology images. However, the scarcity of annotated medical…

Computer Vision and Pattern Recognition · Computer Science 2023-07-24 Héctor Carrión , Narges Norouzi

Optical microscopy has a diffraction limited resolution of about 250 nm. Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are still limited to 10 s of nm, and the images are an indirect pointillist representation of only…

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Reflectance Confocal Microscopy (RCM) is a non-invasive imaging technique used in biomedical research and clinical dermatology. It provides virtual high-resolution images of the skin and superficial tissues, reducing the need for physical…

Image and Video Processing · Electrical Eng. & Systems 2024-04-26 Hong-Jun Yoon , Chris Keum , Alexander Witkowski , Joanna Ludzik , Tracy Petrie , Heidi A. Hanson , Sancy A. Leachman

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically-transparent specimens such as biological samples, but lacks chemical sensitivity limiting its application to…

Optics · Physics 2019-09-04 Miu Tamamitsu , Keiichiro Toda , Ryoichi Horisaki , Takuro Ideguchi

Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable,…

Accurate tumor segmentation and classification in breast ultrasound (BUS) imaging remain challenging due to low contrast, speckle noise, and diverse lesion morphology. This study presents a multi-task deep learning framework that jointly…

Computer Vision and Pattern Recognition · Computer Science 2026-01-12 Samuel E. Johnny , Bernes L. Atabonfack , Israel Alagbe , Assane Gueye

Confocal and multi-photon microscopy are widely used for in-vivo fluorescence imaging of biological tissues such as the brain, offering non-invasive access up to ~1 mm depth without major loss in performance. A recently-developed…

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