Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy
Abstract
Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.
Keywords
Cite
@article{arxiv.2012.08836,
title = {Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy},
author = {Stephanos Yerolatsitis and András Kufcsák and Katjana Ehrlich and Harry A. C. Wood and Susan Fernandes and Tom Quinn and Vikki Young and Irene Young and Katie Hamilton and Ahsan R. Akram and Robert R. Thomson and Keith Finlayson and Kevin Dhaliwal and James M. Stone},
journal= {arXiv preprint arXiv:2012.08836},
year = {2020}
}