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Multimodal and multi-information microscopy techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) extend the informational channels beyond intensity-based fluorescence microscopy but suffer from reduced image quality due to…

Image and Video Processing · Electrical Eng. & Systems 2025-04-15 Hao Chen , Julian Najera , Dagmawit Geresu , Meenal Datta , Cody Smith , Scott Howard

4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail…

Image and Video Processing · Electrical Eng. & Systems 2026-02-17 Cunmin Zhao , Ziyuan Luo , Guoye Guan , Zelin Li , Yiming Ma , Zhongying Zhao , Renjie Wan

Third harmonic generation (THG) provides a valuable, label-free approach to imaging biological systems. To date, THG microscopy has been performed using point scanning methods that rely on intensity measurements lacking phase information of…

Droplet microlasers, as promising tools for biophotonics and biomedical sciences, have witnessed rapid advances due to their flexible reconfigurability, high sensitivity to stimuli, and label-free biosensing ability. However, designing…

High-resolution transmission electron microscopy (HRTEM) is crucial for observing material's structural and morphological evolution at Angstrom scales, but the electron beam can alter these processes. Devices such as CMOS-based…

Materials Science · Physics 2025-12-04 Brian Lee , Meng Li , Judith C Yang , Dmitri N Zakharov , Xiaohui Qu

Mask-based lensless cameras can be flat, thin, and light-weight, which makes them suitable for novel designs of computational imaging systems with large surface areas and arbitrary shapes. Despite recent progress in lensless cameras, the…

Image and Video Processing · Electrical Eng. & Systems 2023-01-11 Yucheng Zheng , M. Salman Asif

Correlative light-electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Most CLEM techniques use…

Aligning light spots into arbitrary shapes is a fundamental challenge in holography, leading to various applications across diverse fields in science and engineering. However, as the spot interval approaches the wavelength of light,…

Lensless optical imaging eliminates the need for refractive optics, enabling compact and low-cost cameras with a large field-of-view, supporting point-of-care diagnostics and industrial monitoring. Practical deployments, however, remain…

When one captures images in low-light conditions, the images often suffer from low visibility. This poor quality may significantly degrade the performance of many computer vision and multimedia algorithms that are primarily designed for…

Computer Vision and Pattern Recognition · Computer Science 2016-07-26 Xiaojie Guo

Motivated by the need for less destructive imaging of nanostructures, we pursue point-source in-line holography (also known as point projection microscopy, or PPM) with very low energy electrons (-100 eV). This technique exploits the recent…

Materials Science · Physics 2015-05-27 Lucian Livadaru , Josh Mutus , Robert A. Wolkow

We present a signal-foreground separation algorithm for filtering observational data to extract spectral distortions of the cosmic microwave background (CMB). Our linear method, called the least response method (LRM), is based on the idea…

Cosmology and Nongalactic Astrophysics · Physics 2024-02-16 J. -P. Maillard , A. Mihalchenko , D. Novikov , A. Osipova , S. Pilipenko , J. Silk

Retinex model has been applied to low-light image enhancement in many existing methods. More appropriate decomposition of a low-light image can help achieve better image enhancement. In this paper, we propose a new pixel-level non-local…

Image and Video Processing · Electrical Eng. & Systems 2021-06-16 Hao Hou , Yingkun Hou , Yuxuan Shi , Benzheng Wei , Jun Xu

Digital holographic microscopy is a powerful tool for characterizing transparent and reflective phase objects. Its ability to reconstruct amplitude and phase can also offer great insight into wavefront shaping and design of all-dielectric…

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

Optics · Physics 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

Visualizing the spatial profile of light beams is essential for evaluating irradiance, characterizing beam quality, and achieving precise alignment. In the optical spectral range, this is readily performed using silicon-based CCD and CMOS…

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

High Dynamic Range (HDR) imaging aims to reproduce the wide range of brightness levels present in natural scenes, which the human visual system can perceive but conventional digital cameras often fail to capture due to their limited dynamic…

Image and Video Processing · Electrical Eng. & Systems 2025-10-28 Kumbha Nagaswetha

We developed a microdisplay-based microscope projection photolithography (MDMPP) technique in which a liquid crystal (LC) microdisplay is used as a reconfigurable photomask for a microscope projector. The LC microdisplay provides a…

Physics Education · Physics 2022-03-23 Young-Gu Ju , Hyeon-Jun Kim , Young-Min Ko

Low light level charge coupled devices (L3CCDs) have recently been developed, incorporating on-chip gain. They may be operated to give an effective readout noise much less than one electron by implementing an on-chip gain process allowing…

Astrophysics · Physics 2009-11-10 A. G. Basden , C. A. Haniff , C. D. Mackay