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One of the primary challenges in live cell culture observation is achieving high-contrast imaging with minimal impact on sample behavior. Quantitative phase imaging (QPI) techniques address this by providing label-free, high-contrast images…

Lensless digital holographic microscopy (LDHM) relies on interference between an unscattered reference wave and a weakly scattered object wave - an assumption that rapidly fails in turbid samples under multiple scattering. To overcome this…

Lensless digital holographic microscopy (LDHM) offers very large field-of-view label-free imaging crucial, e.g., in high-throughput particle tracking and biomedical examination of cells and tissues. Compact layouts promote point-of-case and…

Optics · Physics 2023-10-24 Piotr Arcab , Mikolaj Rogalski , Maciej Trusiak

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Label-Free Multiphoton Microscopy is a very powerful optical microscopy that can be applied to study samples with no need for exogenous fluorescent probes, keeping the main benefits of a Multiphoton approach, like longer penetration depths…

Laser-based lensless digital holographic microscopy (LDHM) is often spoiled by considerable coherent noise factor. We propose a novel LDHM method with significantly limited coherent artifacts, e.g., speckle noise and parasitic interference…

Low-light optical imaging refers to the use of cameras to capture images with minimal photon flux. This area has broad application to diverse fields, including optical microscopy for biological studies. In such studies, it is important to…

This industrial Ph.D. project, carried out in collaboration between Radiometer Medical ApS and SDU Centre for Photonics Engineering at the University of Southern Denmark, explored the use of digital holographic microscopy (DHM) for the…

Optics · Physics 2026-02-10 Andreas Erik Gejl Madsen

Usually, off-axis digital holographic microscopy requires a coherent light source in order to record a full-field hologram. Nevertheless, a LASER-based illumination leads to a non-negligible coherent noise, decreasing then the imaging…

Optics · Physics 2021-11-02 Stephane Perrin , Jonas Kuhn , Christian Depeursinge

Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…

Optics · Physics 2019-04-10 Stephan Amann , Max von Witzleben , and Stefan Breuer

Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…

Optics · Physics 2020-11-04 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

We propose to enhance the performance of localized plasmon structured illumination microscopy (LP-SIM) via intensity correlations. LP-SIM uses sub-wavelength illumination patterns to encode high spatial frequency information. It can enhance…

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

Optics · Physics 2025-06-24 Byung Gyu Chae

Lensless in-line holography is a simple, portable, and cost-effective method of imaging especially for the biomedical microscopy applications. We propose a multiplicative gradient descent optimization based method to obtain multi-depth…

Image and Video Processing · Electrical Eng. & Systems 2020-04-20 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Low-energy electrons (30-250 eV) have been successfully employed for imaging individual biomolecules. The most simple and elegant design of a low-energy electron microscope for imaging biomolecules is a lensless setup that operates in the…

Instrumentation and Detectors · Physics 2014-09-05 Tatiana Latychevskaia , Jean-Nicolas Longchamp , Conrad Escher , Hans-Werner Fink

Low light very likely leads to the degradation of an image's quality and even causes visual task failures. Existing image enhancement technologies are prone to overenhancement, color distortion or time consumption, and their adaptability is…

Image and Video Processing · Electrical Eng. & Systems 2022-05-17 Xiaozhou Lei , Zixiang Fei , Wenju Zhou , Huiyu Zhou , Minrui Fei

Holographic displays are a promising technology for immersive visual experiences, and their potential for compact form factor makes them a strong candidate for head-mounted displays. However, at the short propagation distances needed for a…

Advances in microscopy imaging enable researchers to visualize structures at the nanoscale level thereby unraveling intricate details of biological organization. However, challenges such as image noise, photobleaching of fluorophores, and…

Image and Video Processing · Electrical Eng. & Systems 2024-09-19 Pamela Osuna-Vargas , Maren H. Wehrheim , Lucas Zinz , Johanna Rahm , Ashwin Balakrishnan , Alexandra Kaminer , Mike Heilemann , Matthias Kaschube

Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase…

Optics · Physics 2020-10-28 Michael R Hughes
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