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Endoscopy is the most widely used medical technique for cancer and polyp detection inside hollow organs. However, images acquired by an endoscope are frequently affected by illumination artefacts due to the enlightenment source orientation.…

Image and Video Processing · Electrical Eng. & Systems 2022-07-07 Axel Garcia-Vega , Ricardo Espinosa , Gilberto Ochoa-Ruiz , Thomas Bazin , Luis Eduardo Falcon-Morales , Dominique Lamarque , Christian Daul

Label-free observation of nanoparticles by far-field optical microscopy is challenging because their ability to scatter or absorb light dramatically diminishes with decreasing size. Surface plasmon resonance (SPR) and localized surface…

Optics · Physics 2020-12-02 Nareg Ohannesian , Ibrahim Misbah , Steven H Lin , Wei-Chuan Shih

We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…

Optics · Physics 2021-05-24 Hao Wang , Waleed Tahir , Jiabei Zhu , Lei Tian

A single-pixel digital holography system with phase-encoded illumination using a digital micromirror device (DMD) as a spatial light modulator (SLM) is presented. The enhanced switching rate of DMDs, far exceeding the stringent frame-rate…

A new development in photoacoustic (PA) imaging has been the use of compact, portable and low-cost laser diodes (LDs), but LD-based PA imaging suffers from low signal intensity recorded by the conventional transducers. A common method to…

Image and Video Processing · Electrical Eng. & Systems 2023-03-28 Vincent Vousten , Hamid Moradi , Emad M. Boctor , Septimiu E. Salcudean

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

We propose to use high numerical aperture single mode optical fibers like photonic crystal fiber for lensless in-line holographic microscopy. Highly divergent beam helps to overcome the spatial sampling limitation of the image sensor. In…

Optics · Physics 2020-07-01 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab K. Dutta

Lensless imaging offers a lightweight, compact alternative to traditional lens-based systems, ideal for exploration in space-constrained environments. However, the absence of a focusing lens and limited lighting in such environments often…

Image and Video Processing · Electrical Eng. & Systems 2025-01-14 Ziyang Liu , Tianjiao Zeng , Xu Zhan , Xiaoling Zhang , Edmund Y. Lam

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…

Biological Physics · Physics 2018-08-10 Chiara Garbellotto , Jonathan M. Taylor

Our proposed method of random phase-free holography using virtual convergence light can obtain large reconstructed images exceeding the size of the hologram, without the assistance of random phase. The reconstructed images have low-speckle…

We demonstrate a motion-free intensity diffraction tomography technique that enables direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model,…

Image and Video Processing · Electrical Eng. & Systems 2018-06-26 Ruilong Ling , Waleed Tahir , Hsing-Ying Lin , Hakho Lee , Lei Tian

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

Interaction-free measurement (IFM) has been proposed as a means of high-resolution, low-damage imaging of radiation-sensitive samples, such as biomolecules and proteins. The basic setup for IFM is a Mach-Zehnder interferometer, and recent…

Instrumentation and Detectors · Physics 2019-06-12 Akshay Agarwal , Karl K. Berggren , Vivek Goyal

Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

Multimodal nonlinear endoscopes have been a topic of intense research over the past two decades, enabling sub-cellular and label-free imaging in areas not reachable with table-top microscopes. They are sophisticated systems that can be…

This research presents a novel framework for the compression and decompression of medical images utilizing the Latent Diffusion Model (LDM). The LDM represents advancement over the denoising diffusion probabilistic model (DDPM) with a…

Image and Video Processing · Electrical Eng. & Systems 2023-10-10 InChan Hwang , MinJae Woo

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

High-resolution extreme ultraviolet (XUV) imaging remains limited by conventional approaches that require complex optics such as multilayer mirrors and zone plates. These methods are expensive, suffer from chromatic aberrations and narrow…

Optics · Physics 2025-11-14 Sukyoon Oh , Monalisa Mallick , Thomas Siefke , Christian Spielmann