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The molecular mechanism of the solvent motion that is required to instigate the protein structural relaxation above a critical hydration level or transition temperature has yet to be determined. In this work we use quasi-elastic neutron…

Biomolecules · Quantitative Biology 2016-09-08 Daniela Russo , Greg Hura , Teresa Head-Gordon

Freezing interface interacting with soft cells is a core issue in cryopreservation. However, most of existing cryopreservation approaches face challenges such as complex processing and poor controllability. Herein we report a new,…

Applied Physics · Physics 2020-07-27 Jiaxue You , Yunhan Zhang , Qiao Chen , Yifan Xie , Yixiao Li , Lifang Hu , Li Shang

A recent Monte Carlo simulation determined the potential of mean force between two lysozyme molecules in various aqueous solutions [M. Lund et al. Phys. Rev. Lett. 100, 258105 (2008)]. The study involved a combination of explicit solvent…

Chemical Physics · Physics 2009-11-13 Steven Lettieri , Xiaofei Li , J. D. Gunton

We examine temperature dependent picosecond dynamics as a function of structure and function for lysozyme and cytochrome c using temperature dependent terahertz permittivity measurements. A double Arrhenius temperature dependence with…

Biomolecules · Quantitative Biology 2011-05-24 J. Y. Chen , D. K. George , Yunfen He , J. R. Knab , A. G. Markelz

Ethanol is a common protein crystallization agent, precipitant, and denaturant, but also alters the dielectric properties of solutions. While ethanol-induced unfolding is largely ascribed to its hydrophobic parts, its effect on protein…

Soft Condensed Matter · Physics 2021-11-03 Jan Hansen , Rajeevann Uthayakumar , Jan Skov Pedersen , Stefan U. Egelhaaf , Florian Platten

Intermetallic clathrates are candidate materials for thermoelectric applications above room temperature. Here we explore whether their intrinsically low lattice thermal conductivities can be further reduced by nanostructuring and whether…

Poly(N-isopropylacrylamide) (PNIPAM) is a synthetic polymer that is widely studied for its thermoresponsive character. However, recent works also reported evidence of a low temperature (protein-like) dynamical transition around 225 K in…

Sucralose is a commonly employed artificial sweetener that appears to destabilize protein native structures. This is in direct contrast to the bio-preservative nature of its natural counterpart, sucrose, which enhances the stability of…

We present a detailed dynamic light scattering study on the phase separation in the ocular lens emerging during cold cataract development. Cold cataract is a phase separation effect that proceeds via spinodal decomposition of the lens…

Soft Condensed Matter · Physics 2009-11-13 V. Petta , N. Pharmakakis , G. N. Papatheodorou , S. N. Yannopoulos

The structural evolution of suspensions upon freezing is studied with optical microscopy in a suspended droplet configuration. Droplets have millimeter size and consist in an aqueous mixture of silica particles while the surroundings phase…

Soft Condensed Matter · Physics 2018-05-10 Mathieu Nespoulous , Renaud Denoyel , Mickaël Antoni

A low-temperature dynamical transition has been reported in several proteins. We provide the first observation of a `protein-like' dynamical transition in nonbiological aqueous environments. To this aim we exploit the popular colloidal…

We show that the effect of size dependence of the melting temperature of nanocrystals may be used to govern anomalies of thermodynamic properties of nanocrystals in the premelting range. For example, if temperature of nanocrystals is near…

Materials Science · Physics 2009-10-16 A. I. Karasevskii , V. V. Lubashenko

The nucleation and growth of CdS nanoparticles within a polymer matrix was followed by in-situ synchrotron X-ray diffraction. The nanoparticles form by effect of the thermolysis of thiolate precursors at temperatures between 200 and 300…

Materials Science · Physics 2007-05-23 T. Di Luccio , A. M. Laera , L. Tapfer , S. Kempter , R. Kraus , B. Nickel

We performed molecular dynamics simulations to investigate the viscoelastic properties of aqueous protein solutions containing an antifreeze protein, a toxin protein, and bovine serum albumin. These simulations covered a temperature range…

Soft Condensed Matter · Physics 2023-09-19 Dillon F. Hanlon , Ivan Saika-Voivod , M. Shajahan G. Razul , G. Todd Andrews

We report a dynamic light scattering study on protein suspensions of bovine lens homogenates at conditions (pH and ionic strength) similar to the physiological ones. Light scattering data were collected at two temperatures, 20 oC and 37 oC,…

Soft Condensed Matter · Physics 2009-11-13 A. Giannopoulou , A. J. Aletras , N. Pharmakakis , G. N. Papatheodorou , S. N. Yannopoulos

Employing X-ray photon correlation spectroscopy we measure the kinetics and dynamics of a pressure-induced liquid-liquid phase separation (LLPS) in a water-lysozyme solution. Scattering invariants and kinetic information provide evidence…

Soft Condensed Matter · Physics 2021-07-13 M. Moron , A. Al-Masoodi , C. Lovato , M. Reiser , L. Randolph , G. Surmeier , J. Bolle , F. Westermeier , M. Sprung , R. Winter , M. Paulus , C. Gutt

The protein-polysaccharide combinations that lead to electrostatic complex and coacervates formation are the object of extensive research using both layer-by-layer and mixed emulsion approaches. The protein-polysaccharide conjugates…

Soft Condensed Matter · Physics 2022-01-25 Luigi Gentile

Control of the crystallization process at the microscopic level makes it possible to generate the nanocrystalline samples with the desired structural and morphological properties, that is of great importance for modern industry. In the…

Materials Science · Physics 2019-03-25 Bulat N. Galimzyanov , Dinar T. Yarullin , Anatolii V. Mokshin

The influence of three well-known disaccharides, namely trehalose, maltose and sucrose, on some structural and dynamical properties of lysozyme has been investigated by means of molecular dynamics computer simulations in the 37-60 wt %…

Chemical Physics · Physics 2007-06-12 A. Lerbret , P. Bordat , F. Affouard , A. Hedoux , Y. Guinet , M. Descamps

The formation of ice affects many aspects of our everyday life as well as technologies such as cryotherapy and cryopreservation. Foreign substances almost always aid water freezing through heterogeneous ice nucleation, but the molecular…