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Hydrated proteins undergo a transition in the deeply supercooled regime, which is attributed to rapid changes in hydration water and protein structural dynamics. Here, we investigate the nanoscale stress relaxation in hydrated lysozyme…

Concentrated aqueous solutions of the protein lysozyme undergo a liquid solid transition upon a temperature quench into the unstable spinodal region below a characteristic arrest temperature of Tf=15C. We use video microscopy and…

Soft Condensed Matter · Physics 2015-05-13 Thomas Gibaud , Peter Schurtenberger

Drying colloidal droplets have a wide range of applications from medical diagnostics to coatings for industries. This paper explores the effects of the substrate temperature (ranging from $25$ to $55 ^{\circ}$C) and various initial…

Soft Condensed Matter · Physics 2021-05-14 Anusuya Pal , Amalesh Gope , Germano S. Iannacchione

The study of protein interactions has generated great interest in the food industry. Therefore, research on new supramolecular structures shows promise. Supramolecular structures of the whey proteins {\alpha}-lactalbumin and…

We report theoretical and simulation studies of phase coexistence in model globular protein solutions, based on short-range, central, pair potential representations of the interaction among macro-particles. After reviewing our previous…

Soft Condensed Matter · Physics 2009-11-10 G. Pellicane , D. Costa , C. Caccamo

We report that protein confinement within nanoscopic vesicular compartments corresponds to a liquid-liquid phase transition with the protein/water within vesicle lumen interacting very differently than in bulk. We show this effect leads to…

Pressure provides a powerful parameter to control the protein conformation state, which at sufficiently high values can lead to unfolding. Here, we investigate the effects of increasing pressure up to $0.4$ GPa on hydrated lysozyme…

Studying the freezing of saltwater on a molecular level is of fundamental importance for improving freeze desalination techniques. Here, we investigate the freezing process of NaCl solutions using a combination of x-ray diffraction and…

Chemical Physics · Physics 2020-04-22 Ifigeneia Tsironi , Daniel Schlesinger , Alexander Späh , Lars Eriksson , Mo Segad , Fivos Perakis

A quantitative understanding of aggregation mechanisms leading to the formation of inorganic nanoparticles (NPs) and protein composites in aqueous media is of paramount interest for colloid chemistry. In particular, the interactions between…

In this work, an improved methodology for studying interactions of proteins in solution by small-angle scattering, is presented. Unlike the most common approach, where the protein-protein correlation functions $g_{ij}(r)$ are approximated…

Statistical Mechanics · Physics 2009-11-07 Francesco Spinozzi , Domenico Gazzillo , Achille Giacometti , Paolo Mariani , Flavio Carsughi

The dynamics of a folded protein is studied in water and glycerol at a series of temperatures below and above their respective dynamical transition. The system is modeled in two distinct states whereby the protein is decoupled from the bulk…

Soft Condensed Matter · Physics 2008-09-23 C. Atilgan , A. O. Aykut , A. R. Atilgan

Small angle neutron scattering intensity distributions taken from cytochrome C and lysozyme protein solutions show a rising intensity at very small wave vector, Q, which can be interpreted in terms of the presence of a weak long-range…

Soft Condensed Matter · Physics 2009-11-11 Yun Liu , Emiliano Fratini , Piero Baglioni , Wei-Ren Chen , Sow-Hsin Chen

We investigate theoretically the fluid-crystal coexistence of solutions of globular charged nanoparticles like proteins and inorganic colloids. The thermodynamic properties of the fluid phase are computed via the optimized Baxter model.…

Soft Condensed Matter · Physics 2009-11-11 Peter Prinsen , Theo Odijk

We present the frequency- and temperature-dependent dielectric properties of lysozyme solutions in a broad concentration regime, measured at subzero temperatures and compare the results with measurements above the freezing point of water…

Soft Condensed Matter · Physics 2015-10-01 M. Wolf , S. Emmert , R. Gulich , P. Lunkenheimer , A. Loidl

Water and glycerol are well-known to facilitate the structural relaxation of amorphous protein matrices. However, several studies evidenced that they may also limit fast ($\sim$ pico-nanosecond, ps-ns) and small-amplitude ($\sim$ \AA )…

Soft Condensed Matter · Physics 2017-10-03 Adrien Lerbret , Frédéric Affouard

Glutaraldehyde (GA) reacts with amino groups in proteins, forming intermolecular cross-links that, at sufficiently high protein concentration, can transform a protein solution into a gel. Although GA has been used as a cross-linking reagent…

Chemical Physics · Physics 2017-09-13 Shuji Kaieda , Tomás S. Plivelic , Bertil Halle

In this paper we report structural and optical properties of Magnesium substituted Zinc Oxide (Zn1-xMgxO) nanocrystals (~10-12nm) synthesized by low temperature route. In the low temperature synthesis route it was possible to reach x = 0.17…

Materials Science · Physics 2011-09-15 Manoranjan Ghosh , A. K. Raychaudhuri

Vitrification during cryopreservation requires a detailed understanding of the dynamic behavior of biological solutions. We investigate ferritin diffusion in glycerol-water mixtures at supercooled temperatures using X-ray Photon Correlation…

The effects of static electric field on the dynamics of lysozyme and its hydration water have been investigated by means of incoherent quasi-elastic neutron scattering (QENS). Measurements were performed on lysozyme samples, hydrated…

Soft Condensed Matter · Physics 2013-12-09 P. M. Favi , Q. Zhang , H. O'Neill , E. Mamontov , S. O. Diallo

We use computer simulation to study crystal-forming model proteins equipped with interactions that are both orientationally specific and nonspecific. Distinct dynamical pathways of crystal formation can be selected by tuning the strengths…

Soft Condensed Matter · Physics 2012-04-16 Stephen Whitelam
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