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Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such…

Optics · Physics 2021-01-05 Sharika Mohanan , Alexander D. Corbett

Remote focusing is widely used in 3D two-photon microscopy and 3D photostimulation because it enables fast axial scanning without moving the objective lens or specimen. However, due to the design constraints of microscope optics, remote…

Oblique plane microscopy is a method enabling light-sheet fluorescence imaging through a single microscope objective lens by focusing on a tilted plane within the sample. To focus the fluorescence emitted by the oblique plane on a camera,…

Multichannel, infinite-conjugate optical systems easily allow implementation of multiple image paths and imaging modes into a single microscope. Traditional optical alignment methods which rely on additional hardware are not always simple…

Optics · Physics 2024-07-16 Gemma S. Cairns , Brian R. Patton

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

Instrumentation and Detectors · Physics 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of the residual spherical…

Optics · Physics 2023-05-17 Sharika Mohanan , Alexander D. Corbett

The axis tilt of light beam in optical system would introduce the dispersion of orbital angular momentum (OAM) spectrum. To deal with it, a two-step method is proposed and demonstrated. First, the tilt angle of optical axis is identified…

Optics · Physics 2020-04-27 Peng Zhao , Shikang Li , Yu Wang , Xue Feng , Kaiyu Cui , Fang Liu , Wei Zhang , Yidong Huang

Off-axis parabolic mirrors (OAPs) are occasionally desirable for specialized applications, but are known to introduce field-dependent astigmatic aberrations. In an experiment where optical tweezers are formed by OAPs, another OAP is added…

Optics · Physics 2025-11-20 Meimei Liu , Yuqi Zhu , Giorgio Gratta

In the rapidly evolving field of optical engineering, precise alignment of multi-lens imaging systems is critical yet challenging, as even minor misalignments can significantly degrade performance. Traditional alignment methods rely on…

Optics · Physics 2025-07-01 Tomer Slor , Dean Oren , Shira Baneth , Tom Coen , Haim Suchowski

Selective plane illumination microscopy (SPIM) is an optical sectioning imaging approach based on orthogonal light pathways for excitation and detection. The excitation pathway has an inverse relation between the optical sectioning strength…

Optics · Physics 2024-07-29 Steven J. Sheppard , Peter T. Brown , Douglas P. Shepherd

Current optoacoustic microscopy configurations often have narrow focal ranges that limit their use for fast volumetric imaging applications. Here, the focal range of optoacoustic microscopes is extended by matching the elongated optical…

Optics · Physics 2021-04-15 Zakiullah Ali , Christian Zakian , Vasilis Ntziachristos

Remote focusing of light in a graded-index medium via mode interference is demonstrated using exact analytical solutions of the wave equation. Strong focusing of light occurs at extremely long distances and it revivals periodically with…

Optics · Physics 2015-12-09 Nikolai I. Petrov

In this work, we measure and model tilted x-ray refractive lenses to investigate their effects on an x-ray beam. The modelling is benchmarked against at-wavelength metrology obtained with x-ray speckle vector tracking experiments (XSVT) at…

Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration. However, axial distances can be distorted by both the point spread function and by a…

Soft Condensed Matter · Physics 2014-04-16 Thijs Herman Besseling , Jissy Jose , Alfons van Blaaderen

TianQin is a planned Chinese space-based gravitational wave (GW) observatory with a frequency band of 10-4 to 1Hz. Optical telescopes are essential for the delivery of the measurement beam to support a precise distance measurement between…

Instrumentation and Methods for Astrophysics · Physics 2023-03-07 Zichao Fan , Zhengbo Zhu , Huiru Ji , Yan Mo , Hao Tan , Lujia Zhao , Shengyi Cao , Donglin Ma

Modern extreme adaptive optics (AO) systems achieving diffraction-limited performance open up new possibilities for instrumentation. Especially important for the fields of spectroscopy and interferometry is that it enables the prospect to…

Angular differential imaging (ADI) (Marois et al. 2006) is an observational technique in high contrast imaging where the telescope is used in pupil tracking mode so that the image of the sky rotates with respect to the optical surfaces.…

Earth and Planetary Astrophysics · Physics 2017-11-28 Michael Bottom , Garreth Ruane , Dimitri Mawet

The far-field resolution of optical imaging systems is restricted by the Abbe diffraction limit, a direct result of the wave nature of light. One successful technological approach to circumventing this limit is to reduce the effective size…

Adaptive optics (AO) have been used to correct wavefronts to achieve diffraction limited point spread functions in a broad range of optical applications, prominently ground-based astronomical telescopes operating in near infra-red. While…

Instrumentation and Methods for Astrophysics · Physics 2018-10-03 Alexandre J. T. S. Mello , Antonin H. Bouchez , Andrew Szentgyorgyi , Marcos A. van Dam , Henrique Lupinari

Rather than using an adaptive optics (AO) system to correct a telescope s entire pupil, it can instead be used to more finely correct a smaller sub-aperture. Indeed, existing AO systems can be used to correct a sub-aperture 1/3 to 1/2 the…

Astrophysics · Physics 2009-11-13 E. Serabyn , K. Wallace , M. Troy , B. Mennesson , P. Haguenauer , R. Gappinger , R. Burruss
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