English
Related papers

Related papers: Super-resolved FRET imaging by confocal fluorescen…

200 papers

Single-beam scanning microscopy (SBSM) is one of the most robust strategies for commercial optical systems. Although structured illumination combined with Fourier-domain spatial spectrum fusion can enhance SBSM resolution beyond the…

Optics · Physics 2025-07-15 Yongtao Liu , Tianxiao Wu , Xiao Zhou , Fan Wang

Kernel-phase is a recently developed paradigm that tackles the classical problem of image deconvolution, based on an interferometric point of view of image formation. Kernel-phase inherits and borrows from the notion of closure-phase,…

Instrumentation and Methods for Astrophysics · Physics 2015-06-17 Frantz Martinache

Coherent diffractive imaging of individual free nanoparticles has opened novel routes for the in-situ analysis of their transient structural, optical, and electronic properties. So far, single-shot single-particle diffraction was assumed to…

Current Flash X-ray single-particle diffraction Imaging (FXI) experiments, which operate on modern X-ray Free Electron Lasers (XFELs), can record millions of interpretable diffraction patterns from individual biomolecules per day. Due to…

Computer Vision and Pattern Recognition · Computer Science 2019-02-20 Jing Liu , Gijs van der Schot , Stefan Engblom

We propose a method of spatial resolution enhancement in metrology (thermometry, magnetometry, pH estimation, and similar methods) with blinking fluorescent nanosensors by combining sensing with super-resolution optical fluctuation imaging…

Optics · Physics 2026-05-11 Alexander Mikhalychev , Alex Ulyanenkov

We demonstrate fluorescence microscopy of individual fermionic potassium atoms in a 527-nm-period optical lattice. Using electromagnetically induced transparency (EIT) cooling on the 770.1-nm D$_1$ transition of $^{40}$K, we find that atoms…

Quantum Gases · Physics 2015-12-15 G. J. A. Edge , R. Anderson , D. Jervis , D. C. McKay , R. Day , S. Trotzky , J. H. Thywissen

SPAD cameras offer single photon detection sensitivity, high frame rates and zero readout noise. They are a core technology for widefield FLIM, but have further potential in ultra-fast imaging applications. However, in practice sensitivity…

Instrumentation and Detectors · Physics 2016-02-15 Istvan Gyongy , Amy Davies , Neale A. W. Dutton , Rory Duncan , Colin Rickman , Robert K. Henderson , Paul Dalgarno

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…

We show that de-focused single particle images recorded using a cryo-electron microscope (cryoEM) system may be processed like a Fresnel zone in-line hologram to obtain physically meaningful quantitative phase maps associated with…

Image and Video Processing · Electrical Eng. & Systems 2021-11-24 Apoorv Pant , Manidipa Banerjee , Kedar Khare

Single molecule time traces reveal the time evolution of unsynchronized kinetic systems. Especially single molecule F\"orster resonance energy transfer (smFRET) provides access to enzymatically important timescales, combined with molecular…

Quantitative Methods · Quantitative Biology 2018-01-17 Sonja Schmid , Thorsten Hugel

Microscopy has been key to tremendous advances in science, technology, and medicine, revealing structure and dynamics across time and length scales. However, combining high spatial and temporal resolution in a non-invasive, label-free…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

A major challenge in many modern superresolution fluorescence microscopy techniques at the nanoscale lies in the correct alignment of long sequences of sparse but spatially and temporally highly resolved images. This is caused by the…

Atom probe tomography (APT) helps elucidate the link between the nanoscale chemical variations and physical properties, but it has limited structural resolution. Field ion microscopy (FIM), a predecessor technique to APT, is capable of…

We demonstrate single-site-resolved fluorescence imaging of ultracold $^{87}\mathrm{Rb}$ atoms in a triangular optical lattice by employing Raman sideband cooling. Combining a Raman transition at the D1 line and a photon scattering through…

Quantum Gases · Physics 2020-12-22 Ryuta Yamamoto , Hideki Ozawa , David C. Nak , Ippei Nakamura , Takeshi Fukuhara

Single-atom-resolved detection in optical lattices using quantum-gas microscopes has enabled a new generation of experiments in the field of quantum simulation. Fluorescence imaging of individual atoms has so far been achieved for bosonic…

Accelerating imaging speed in optical microscopy is often realized at the expense of image contrast, image resolution, and detection sensitivity- a common predicament for advancing high-speed and high-throughput cellular imaging. We here…

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

In this work, we report a confocal laser induced fluorescence (LIF) configuration, which allows for high spatial resolution measurements of plasma properties in plasma setups and sources with a limited optical access. The proposed LIF…

Plasma Physics · Physics 2023-07-26 I. Romadanov , Y. Raitses

MINFLUX (Minimal Photon Flux) is a single-molecule imaging technique capable of resolving fluorophores at a precision of <5 nm. Interpretation of the point patterns generated by this technique presents challenges due to variable emitter…

Applications · Statistics 2026-02-24 Jack Peyton , Benjamin Davis , Emily Gribbin , Daniel Rolfe , Hannah Mitchell