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Universal, transferable whole-slide image (WSI) representations are central to computational pathology. Incorporating multiple markers (e.g., immunohistochemistry, IHC) alongside H&E enriches H&E-based features with diverse, biologically…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yizhi Zhang , Lei Fan , Zhulin Tao , Donglin Di , Yang Song , Sidong Liu , Cong Cong

Separating the contributions of individual chromogenic stains in RGB histology whole slide images (WSIs) is essential for stain normalization, quantitative assessment of marker expression, and cell-level readouts in immunohistochemistry…

Computer Vision and Pattern Recognition · Computer Science 2026-01-19 Mark Eastwood , Thomas McKee , Zedong Hu , Sabine Tejpar , Fayyaz Minhas

Computer-aided diagnosis (CAD) can be used as an important tool to aid and enhance pathologists' diagnostic decision-making. Deep learning techniques, such as convolutional neural networks (CNN) and fully convolutional networks (FCN), have…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Dalí F. D. dos Santos , Paulo R. de Faria , Adriano M. Loyola , Sérgio V. Cardoso , Bruno A. N. Travençolo , Marcelo Z. do Nascimento

Pathologists routinely use immunohistochemical (IHC)-stained tissue slides against MelanA in addition to hematoxylin and eosin (H&E)-stained slides to improve their accuracy in diagnosing melanomas. The use of diagnostic Deep Learning…

The rapid development of digital pathology and modern deep learning has facilitated the emergence of pathology foundation models that are expected to solve general pathology problems under various disease conditions in one unified model,…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Yutong Sun , Sichen Zhu , Peng Qiu

Conditional generative adversarial networks (cGANs) have enabled high-fidelity computational staining and destaining of hematoxylin and eosin (H&E) in digital pathology whole-slide images (WSI). However, their ability to generalize to…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Aarushi Kulkarni , Alarice Lowe , Pratik Shah

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

Computer Vision and Pattern Recognition · Computer Science 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

Immunohistochemical (IHC) staining highlights the molecular information critical to diagnostics in tissue samples. However, compared to H&E staining, IHC staining can be much more expensive in terms of both labor and the laboratory…

Computer Vision and Pattern Recognition · Computer Science 2023-03-14 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

Computer assisted diagnosis in digital pathology is becoming ubiquitous as it can provide more efficient and objective healthcare diagnostics. Recent advances have shown that the convolutional Neural Network (CNN) architectures, a…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Sruthi Krishna , Suganthi S. S , Shivsubramani Krishnamoorthy , Arnav Bhavsar

Increased levels of tumor infiltrating lymphocytes (TILs) in cancer tissue indicate favourable outcomes in many types of cancer. Manual quantification of immune cells is inaccurate and time consuming for pathologists. Our aim is to leverage…

Histopathology slides are routinely marked by pathologists using permanent ink markers that should not be removed as they form part of the medical record. Often tumour regions are marked up for the purpose of highlighting features or other…

Computer Vision and Pattern Recognition · Computer Science 2019-05-14 Sharib Ali , Nasullah Khalid Alham , Clare Verrill , Jens Rittscher

Histopathological evaluation of tissue samples is a key practice in patient diagnosis and drug development, especially in oncology. Historically, Hematoxylin and Eosin (H&E) has been used by pathologists as a gold standard staining.…

Computer Vision and Pattern Recognition · Computer Science 2018-10-16 Amal Lahiani , Jacob Gildenblat , Irina Klaman , Shadi Albarqouni , Nassir Navab , Eldad Klaiman

Holographic microscopy presents challenges for color reproduction due to the usage of narrow-band illumination sources, which especially impacts the imaging of stained pathology slides for clinical diagnoses. Here, an accurate color…

Medical Physics · Physics 2019-03-06 Yibo Zhang , Tairan Liu , Yujia Huang , Da Teng , Yinxu Bian , Yichen Wu , Yair Rivenson , Alborz Feizi , Aydogan Ozcan

Chemical staining methods are dependable but require extensive time, expensive chemicals, and raise environmental concerns. These challenges highlight the need for alternative solutions like virtual staining, which accelerates the…

Image and Video Processing · Electrical Eng. & Systems 2024-07-02 Mehdi Ounissi , Ilias Sarbout , Jean-Pierre Hugot , Christine Martinez-Vinson , Dominique Berrebi , Daniel Racoceanu

Medical practitioners use a number of diagnostic tests to make a reliable diagnosis. Traditionally, Haematoxylin and Eosin (H&E) stained glass slides have been used for cancer diagnosis and tumor detection. However, recently a variety of…

Image and Video Processing · Electrical Eng. & Systems 2023-12-27 Abubakr Shafique , Morteza Babaie , Ricardo Gonzalez , H. R. Tizhoosh

Color inconsistency is an inevitable challenge in computational pathology, which generally happens because of stain intensity variations or sections scanned by different scanners. It harms the pathological image analysis methods, especially…

Image and Video Processing · Electrical Eng. & Systems 2022-03-01 Bingchao Zhao , Jiatai Lin , Changhong Liang , Zongjian Yi , Xin Chen , Bingbing Li , Weihao Qiu , Danyi Li , Li Liang , Chu Han , Zaiyi Liu

This study systematically investigates the impact of image enhancement techniques on Convolutional Neural Network (CNN)-based Brain Tumor Segmentation, focusing on Histogram Equalization (HE), Contrast Limited Adaptive Histogram…

Image and Video Processing · Electrical Eng. & Systems 2024-04-09 Shoffan Saifullah , Andri Pranolo , Rafał Dreżewski

Stain variation is a unique challenge associated with automated analysis of digital pathology. Numerous methods have been developed to improve the robustness of machine learning methods to stain variation, but comparative studies have…

Image and Video Processing · Electrical Eng. & Systems 2023-11-14 Michael Yeung , Todd Watts , Sean YW Tan , Pedro F. Ferreira , Andrew D. Scott , Sonia Nielles-Vallespin , Guang Yang

Automatization of the diagnosis of any kind of disease is of great importance and it's gaining speed as more and more deep learning solutions are applied to different problems. One of such computer aided systems could be a decision support…

Computer Vision and Pattern Recognition · Computer Science 2018-04-24 Tomas Iesmantas , Robertas Alzbutas

Generalization is one of the main challenges of computational pathology. Slide preparation heterogeneity and the diversity of scanners lead to poor model performance when used on data from medical centers not seen during training. In order…

Image and Video Processing · Electrical Eng. & Systems 2024-01-09 Nicolas Nerrienet , Rémy Peyret , Marie Sockeel , Stéphane Sockeel
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