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The automatic registration of noninvasive second-harmonic generation microscopy to hematoxylin and eosin slides is a highly desired, yet still unsolved problem. The task is challenging because the second-harmonic images contain only partial…

Computer Vision and Pattern Recognition · Computer Science 2024-09-25 Marek Wodzinski , Henning Müller

According to GLOBOCAN 2020, prostate cancer is the second most common cancer in men worldwide and the fourth most prevalent cancer overall. For pathologists, grading prostate cancer is challenging, especially when discriminating between…

Image and Video Processing · Electrical Eng. & Systems 2023-03-22 Zahra Tabatabaei , Adrian colomer , Kjersti Engan , Javier Oliver , Valery Naranjo

Immunohistochemical (IHC) staining serves as a valuable technique for detecting specific antigens or proteins through antibody-mediated visualization. However, the IHC staining process is both time-consuming and costly. To address these…

Computer Vision and Pattern Recognition · Computer Science 2025-04-02 Zetong Chen , Yuzhuo Chen , Hai Zhong , Xu Qiao

The popular use of histopathology images, such as hematoxylin and eosin (H&E), has proven to be useful in detecting tumors. However, moving such cancer cases forward for treatment requires accurate on the amount of the human epidermal…

Computer Vision and Pattern Recognition · Computer Science 2025-12-30 Olaide N. Oyelade , Oliver Hoxey , Yulia Humrye

Histopathology image analysis is fundamental to digital pathology, with hematoxylin and eosin (H&E) staining as the gold standard for diagnostic and prognostic assessments. While H&E imaging effectively highlights cellular and tissue…

Image and Video Processing · Electrical Eng. & Systems 2025-11-18 Yao Du , Jiaxin Zhuang , Xiaoyu Zheng , Jing Cong , Limei Guo , Chao He , Lin Luo , Xiaomeng Li

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

Histopathological images provide rich information for disease diagnosis. Large numbers of histopathological images have been digitized into high resolution whole slide images, opening opportunities in developing computational image analysis…

Image and Video Processing · Electrical Eng. & Systems 2020-11-06 Jiayun Li , Wenyuan Li , Anthony Sisk , Huihui Ye , W. Dean Wallace , William Speier , Corey W. Arnold

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Confocal laser endomicroscopy (CLE) allow on-the-fly in vivo intraoperative imaging in a discreet field of view, especially for brain tumors, rather than extracting tissue for examination ex vivo with conventional light microscopy.…

Background and Purpose: Colorectal cancer is a common fatal malignancy, the fourth most common cancer in men, and the third most common cancer in women worldwide. Timely detection of cancer in its early stages is essential for treating the…

Purpose: In digital histopathology, virtual multi-staining is important for diagnosis and biomarker research. Additionally, it provides accurate ground-truth for various deep-learning tasks. Virtual multi-staining can be obtained using…

Image and Video Processing · Electrical Eng. & Systems 2023-12-15 Johannes Lotz , Nick Weiss , Jeroen van der Laak , Stefan Heldmann

Histopathology relies on the analysis of microscopic tissue images to diagnose disease. A crucial part of tissue preparation is staining whereby a dye is used to make the salient tissue components more distinguishable. However, differences…

Image and Video Processing · Electrical Eng. & Systems 2022-08-03 Haseeb Nazki , Ognjen Arandjelović , InHwa Um , David Harrison

The development of reliable imaging biomarkers for the analysis of colorectal cancer (CRC) in hematoxylin and eosin (H&E) stained histopathology images requires an accurate and reproducible classification of the main tissue components in…

Breast Cancer is a major cause of death worldwide among women. Hematoxylin and Eosin (H&E) stained breast tissue samples from biopsies are observed under microscopes for the primary diagnosis of breast cancer. In this paper, we propose a…

Computer Vision and Pattern Recognition · Computer Science 2018-07-26 Aditya Golatkar , Deepak Anand , Amit Sethi

Synthetic images can be used for the development and evaluation of deep learning algorithms in the context of limited availability of data. In the field of computational pathology, where histology images are large in size and visual context…

Image and Video Processing · Electrical Eng. & Systems 2021-03-29 Srijay Deshpande , Fayyaz Minhas , Simon Graham , Nasir Rajpoot

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

Image and Video Processing · Electrical Eng. & Systems 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

We propose a novel hematoxylin and eosin (H&E) stain normalization method based on a modified U-Net neural network architecture. Unlike previous deep-learning methods that were often based on generative adversarial networks (GANs), we take…

Image and Video Processing · Electrical Eng. & Systems 2022-11-11 Chi-Chen Lee , Po-Tsun Paul Kuo , Chi-Han Peng

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

Image and Video Processing · Electrical Eng. & Systems 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

In addition to evaluating tumor morphology using H&E staining, immunohistochemistry is used to assess the presence of specific proteins within the tissue. However, this is a costly and labor-intensive technique, for which virtual staining,…

Computer Vision and Pattern Recognition · Computer Science 2025-11-25 José Teixeira , Pascal Klöckner , Diana Montezuma , Melis Erdal Cesur , João Fraga , Hugo M. Horlings , Jaime S. Cardoso , Sara P. Oliveira