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Related papers: H&E Stain Normalization using U-Net

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Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

The variation in histologic staining between different medical centers is one of the most profound challenges in the field of computer-aided diagnosis. The appearance disparity of pathological whole slide images causes algorithms to become…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Martin J. Hetz , Tabea-Clara Bucher , Titus J. Brinker

The rapid growth of digital pathology in recent years has provided an ideal opportunity for the development of artificial intelligence-based tools to improve the accuracy and efficiency of clinical diagnoses. One of the significant…

Image and Video Processing · Electrical Eng. & Systems 2024-03-08 Jack Breen , Kieran Zucker , Katie Allen , Nishant Ravikumar , Nicolas M. Orsi

Histopathological diagnoses of tumors in tissue biopsy after Hematoxylin and Eosin (H&E) staining is the gold standard for oncology care. H&E staining is slow and uses dyes, reagents and precious tissue samples that cannot be reused.…

Variations in hematoxylin and eosin (H&E) stained images (due to clinical lab protocols, scanners, etc) directly impact the quality and accuracy of clinical diagnosis, and hence it is important to control for these variations for a reliable…

Image and Video Processing · Electrical Eng. & Systems 2020-06-26 Saad Nadeem , Travis Hollmann , Allen Tannenbaum

In practice, digital pathology images are often affected by various factors, resulting in very large differences in color and brightness. Stain normalization can effectively reduce the differences in color and brightness of digital…

Image and Video Processing · Electrical Eng. & Systems 2024-07-17 Hongtao Kang , Die Luo , Li Chen , Junbo Hu , Tingwei Quan , Shaoqun Zeng , Shenghua Cheng , Xiuli Liu

Double staining in histopathology, particularly for metaplastic breast cancer, typically employs H&E and P63 dyes. However, P63's tissue damage and high cost necessitate alternative methods. This study introduces xAI-CycleGAN, an advanced…

Image and Video Processing · Electrical Eng. & Systems 2023-12-07 Tibor Sloboda , Lukáš Hudec , Wanda Benešová

In digital pathology, many image analysis tasks are challenged by the need for large and time-consuming manual data annotations to cope with various sources of variability in the image domain. Unsupervised domain adaptation based on…

Image and Video Processing · Electrical Eng. & Systems 2022-05-18 Nassim Bouteldja , Barbara Mara Klinkhammer , Tarek Schlaich , Peter Boor , Dorit Merhof

Immunohistochemical (IHC) staining serves as a valuable technique for detecting specific antigens or proteins through antibody-mediated visualization. However, the IHC staining process is both time-consuming and costly. To address these…

Computer Vision and Pattern Recognition · Computer Science 2025-04-02 Zetong Chen , Yuzhuo Chen , Hai Zhong , Xu Qiao

Computer assisted diagnosis in digital pathology is becoming ubiquitous as it can provide more efficient and objective healthcare diagnostics. Recent advances have shown that the convolutional Neural Network (CNN) architectures, a…

Image and Video Processing · Electrical Eng. & Systems 2022-01-05 Sruthi Krishna , Suganthi S. S , Shivsubramani Krishnamoorthy , Arnav Bhavsar

Hematoxylin and eosin (H&E) staining visualizes histology but lacks specificity for diagnostic markers. Immunohistochemistry (IHC) staining provides protein-targeted staining but is restricted by tissue availability and antibody…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Anran Liu , Xiaofei Wang , Jing Cai , Chao Li

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-28 Shikha Dubey , Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Histopathology relies on the analysis of microscopic tissue images to diagnose disease. A crucial part of tissue preparation is staining whereby a dye is used to make the salient tissue components more distinguishable. However, differences…

Image and Video Processing · Electrical Eng. & Systems 2022-08-03 Haseeb Nazki , Ognjen Arandjelović , InHwa Um , David Harrison

The automated analysis of medical images is currently limited by technical and biological noise and bias. The same source tissue can be represented by vastly different images if the image acquisition or processing protocols vary. For an…

In whole slide imaging, commonly used staining techniques based on hematoxylin and eosin (H&E) and immunohistochemistry (IHC) stains accentuate different aspects of the tissue landscape. In the case of detecting metastases, IHC provides a…

Image and Video Processing · Electrical Eng. & Systems 2022-08-30 Joseph Boyd , Irène Villa , Marie-Christine Mathieu , Eric Deutsch , Nikos Paragios , Maria Vakalopoulou , Stergios Christodoulidis

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Virtual stain transfer is a promising area of research in Computational Pathology, which has a great potential to alleviate important limitations when applying deeplearningbased solutions such as lack of annotations and sensitivity to a…

Computer Vision and Pattern Recognition · Computer Science 2022-10-19 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

Compared to hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) not only maintains the structural features of tissue samples, but also provides high-resolution protein localization, which is essential for aiding in pathology…

Computer Vision and Pattern Recognition · Computer Science 2025-11-04 Yuhang Kang , Ziyu Su , Tianyang Wang , Zaibo Li , Wei Chen , Muhammad Khalid Khan Niazi

Staining is critical to cell imaging and medical diagnosis, which is expensive, time-consuming, labor-intensive, and causes irreversible changes to cell tissues. Recent advances in deep learning enabled digital staining via supervised model…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Ziwang Xu , Lanqing Guo , Shuyan Zhang , Alex C. Kot , Bihan Wen

Conditional generative adversarial networks (cGANs) have enabled high-fidelity computational staining and destaining of hematoxylin and eosin (H&E) in digital pathology whole-slide images (WSI). However, their ability to generalize to…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Aarushi Kulkarni , Alarice Lowe , Pratik Shah