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X-ray single particle imaging (SPI) has offered the potential to visualize structures of biomolecules at near-atomic resolution. However, state-of-the-art structures at X-ray free electron lasers (XFELs) are limited to moderate resolution,…

Optics · Physics 2025-08-12 Abhishek Mall , Zhou Shen , Kartik Ayyer

The development of X-ray Free Electron Lasers (XFELs) has opened numerous opportunities to probe atomic structure and ultrafast dynamics of various materials. Single Particle Imaging (SPI) with XFELs enables the investigation of biological…

Computer Vision and Pattern Recognition · Computer Science 2023-11-29 Zhantao Chen , Cong Wang , Mingye Gao , Chun Hong Yoon , Jana B. Thayer , Joshua J. Turner

X-ray free-electron lasers (XFELs) offer unique capabilities for measuring the structure and dynamics of biomolecules, helping us understand the basic building blocks of life. Notably, high-repetition-rate XFELs enable single particle…

Computer Vision and Pattern Recognition · Computer Science 2023-12-25 Jay Shenoy , Axel Levy , Frédéric Poitevin , Gordon Wetzstein

X-ray single particle imaging involves the measurement of a large number of noisy diffraction patterns of isolated objects in random orientations. The missing information about these patterns is then computationally recovered in order to…

Image and Video Processing · Electrical Eng. & Systems 2020-05-25 Kartik Ayyer

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is particularly well suited to determine the 3D structure of particles in their native environment. For a successful reconstruction, diffraction patterns originating from a…

Data Analysis, Statistics and Probability · Physics 2021-12-17 Dameli Assalauova , Alexandr Ignatenko , Fabian Isensee , Sergey Bobkov , Darya Trofimova , Ivan A. Vartanyants

Single-particle imaging experiments of biomolecules at x-ray free-electron lasers (XFELs) require processing of hundreds of thousands (or more) of images that contain very few x-rays. Each low-flux image of the diffraction pattern is…

Instrumentation and Detectors · Physics 2012-03-16 Hugh T. Philipp , Kartik Ayyer , Mark W. Tate , Veit Elser , Sol M. Gruner

A well-characterised wavefront is important for many X-ray free-electron laser (XFEL) experiments, especially for single-particle imaging (SPI), where individual bio-molecules randomly sample a nanometer-region of highly-focused femtosecond…

Single-pixel imaging (SPI) is a novel imaging technique whose working principle is based on the compressive sensing (CS) theory. In SPI, data is obtained through a series of compressive measurements and the corresponding image is…

Image and Video Processing · Electrical Eng. & Systems 2022-07-15 Stephen L. H. Lau , Edwin K. P. Chong

Single-pixel imaging (SPI) is significant for applications constrained by transmission bandwidth or lighting band, where 3D SPI can be further realized through capturing signals carrying depth. Sampling strategy and reconstruction algorithm…

Image and Video Processing · Electrical Eng. & Systems 2025-04-01 Xinyue Ma , Chenxing Wang

Single-shot wide-angle diffraction imaging is a widely used method to investigate the structure of non-crystallizing objects such as nanoclusters, large proteins or even viruses. Its main advantage is that information about the…

Data Analysis, Statistics and Probability · Physics 2021-06-02 Thomas Stielow , Stefan Scheel

Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized. One of the challenges in processing the data…

Biological Physics · Physics 2013-10-16 O. M. Yefanov , I. A. Vartanyants

Single-Pixel Imaging (SPI) enables the reconstruction of objects using a single detector through sequential illuminations with structured light patterns. The choice of illumination patterns is critical, particularly in highly undersampled…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Serban Cristian Tudosie , Alexander Denker , Zeljko Kereta , Simon Arridge

We propose an encryption-decryption framework for validating diffraction intensity volumes reconstructed using single-particle imaging (SPI) with x-ray free-electron lasers (XFELs) when the ground truth volume is absent. This framework…

Data Analysis, Statistics and Probability · Physics 2020-08-03 Zhou Shen , Colin Zhi Wei Teo , Kartik Ayyer , N. Duane Loh

Single-pixel imaging (SPI) is an emerging technique which has attracts wide attention in various research fields. However, restricted by the low reconstruction quality and large amount of measurements, the practical application is still in…

Image and Video Processing · Electrical Eng. & Systems 2018-11-09 Chao Deng , Xuemei Hu , Xiaoxu Li , Jinli Suo , Zhili Zhang , Qionghai Dai

With X-ray free-electron lasers (XFELs), it is possible to determine the three-dimensional structure of noncrystalline nanoscale particles using X-ray single-particle imaging (SPI) techniques at room temperature. Classifying SPI scattering…

Machine Learning · Computer Science 2023-02-15 Cong Wang , Eric Florin , Hsing-Yin Chang , Jana Thayer , Chun Hong Yoon

We introduce a compressive single-pixel imaging (SPI) framework for high-resolution image capture in fractions of a second. This framework combines a dedicated sampling strategy with a tailored reconstruction method to enable high-quality…

Image and Video Processing · Electrical Eng. & Systems 2025-09-03 Anna Pastuszczak , Rafał Stojek , Piotr Wróbel , Magdalena Cwojdzińska , Kacper Sobczak , Rafał Kotyński

Single particle imaging (SPI) is a promising method for native structure determination which has undergone a fast progress with the development of X-ray Free-Electron Lasers. Large amounts of data are collected during SPI experiments,…

Image and Video Processing · Electrical Eng. & Systems 2021-04-23 Alexandr Ignatenko , Dameli Assalauova , Sergey A. Bobkov , Luca Gelisio , Anton B. Teslyuk , Viacheslav A. Ilyin , Ivan A. Vartanyants

The Linac Coherent Light Source (LCLS) is an X- ray free electron laser (XFEL) facility enabling the study of the structure and dynamics of single macromolecules. A major upgrade will bring the repetition rate of the X-ray source from 120…

Computational Physics · Physics 2021-09-14 Hsing-Yin Chang , Elliott Slaughter , Seema Mirchandaney , Jeffrey Donatelli , Chun Hong Yoon
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