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Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation…

Optical beams and starlight distorted by atmospheric turbulence can be corrected with adaptive optics systems to enable efficient coupling into single-mode fibers. Deformable mirrors, used to flatten the wavefront in astronomical…

Confocal Raman microscopy, a highly specific and label-free technique for the microscale study of thick samples, often presents difficulties due to weak Raman signals. Inhomogeneous samples introduce wavefront aberrations that further…

Optical imaging through scattering media is a fundamental challenge in many applications. Recently, substantial breakthroughs such as imaging through biological tissues and looking around corners have been obtained by the use of…

Optics · Physics 2020-07-09 Tomer Yeminy , Ori Katz

Multi-photon microscopy is a powerful technique for deep-tissue imaging, providing high spatial resolution at increased penetration depth. Nevertheless, imaging remains largely restricted to superficial tissue layers well below 1 mm.…

The highest three-dimensional (3D) resolution possible in in-vivo retinal imaging is achieved by combining optical coherence tomography (OCT) and adaptive optics (AO). However, this combination brings important limitations, such as small…

Medical Physics · Physics 2020-10-21 Jules Scholler , Kassandra Groux , Kate Grieve , Claude Boccara , Pedro Mecê

Wavefront shaping is increasingly being used in modern microscopy to obtain distortion-free, high-resolution images deep inside inhomogeneous media. Wavefront shaping methods typically rely on the presence of a 'guidestar' in order to find…

Optical wavefront-shaping has emerged as a powerful tool to manipulate light in strongly scattering media. It enables diffraction-limited focusing and imaging at depths where conventional microscopy techniques fail. However, while most…

Optics · Physics 2014-03-04 T. Chaigne , O. Katz , A. C. Boccara , M. Fink , E. Bossy , S. Gigan

Reconfigurable photonic devices capable of routing the flow of light enable flexible integrated-optic circuits that are not hard-wired but can be externally controlled. Analogous to free-space spatial light modulators, we demonstrate…

We present a promising approach to the extremely fast sensing and correction of small wavefront errors in adaptive optics systems. As our algorithm's computational complexity is roughly proportional to the number of actuators, it is…

Instrumentation and Methods for Astrophysics · Physics 2016-11-26 Christoph U. Keller , Visa Korkiakoski , Niek Doelman , Rufus Fraanje , Raluca Andrei , Michel Verhaegen

We present a reference-free computational wavefront sensor based on binary amplitude modulation and phase retrieval. The method employs Digital Micro-mirror Device as a programmable amplitude modulator and reconstructs the complex optical…

Optics · Physics 2026-02-12 Ondrej Denk , Jan Pilar , Martin Divoky , Miroslav Cech , Tomas Mocek

Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…

Optics · Physics 2026-04-21 Pablo Jara , Arthur Goetschy , Hui Cao , Alexey Yamilov

Multimode fibers (MMFs) can transmit multiple guided modes simultaneously, making them a promising platform for high-resolution biomedical imaging, endoscopy and high-bandwidth optical communication. However, their complex modal behavior,…

Optics · Physics 2026-05-28 Dvir Marsh , Lior Fridman , Stav Lotan , Amit Kam , Shie Mannor , Guy Bartal

Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…

Images obtained by microscopes are generally degraded by aberrations. Adaptive optics (AO) has been widely used in to compensate for aberrations and improve reduced image quality. Requiring no separate wavefront sensor, sensorless AO…

Optics · Physics 2025-09-23 Biwei Zhang , Qi Hu , Jingyu Wang , Martin J. Booth

Wavefront shaping systems aim to image deep into scattering tissue by reshaping incoming and outgoing light to correct aberrations caused by tissue inhomogeneity However, the desired modulation depends on the unknown tissue structure and…

Optics · Physics 2026-01-14 Sagi Monin , Marina Alterman , Anat Levin

One of the fundamental limitations in photonics is the lack of a bidirectional transducer that can convert optical information into electronic signals or vice versa. In acoustics or at microwave frequencies, wave signals can be…

Optics · Physics 2019-08-28 Seungwoo Shin , KyeoReh Lee , YoonSeok Baek , YongKeun Park

We present a novel learning-based method to build a differentiable computational model of a real fluorescence microscope. Our model can be used to calibrate a real optical setup directly from data samples and to engineer point spread…

Image and Video Processing · Electrical Eng. & Systems 2023-06-12 Josue Page , Paolo Favaro
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