Related papers: Predicting cell stress and strain during extrusion…
In the emerging field of 3D bioprinting, cell damage due to large deformations is considered a main cause for cell death and loss of functionality inside the printed construct. Those deformations, in turn, strongly depend on the…
A novel piezoelectric, drop-on-demand (DOD) inkjet system has been developed and used to print L929 rat fibroblast cells. We investigate the survivability of the cells subjected to the large stresses during the printing process. These…
Animal cells in tissues are supported by biopolymer matrices, which typically exhibit highly nonlinear mechanical properties. While the linear elasticity of the matrix can significantly impact cell mechanics and functionality, it remains…
Bioprinting is an enabling biofabrication technique to create heterogeneous tissue constructs according to patient-specific geometries and compositions. Optimization of bioinks as per requirements for specific tissue applications is a…
Cell layers eliminate unwanted cells through the extrusion process, which underlines healthy versus flawed tissue behaviors. Although several biochemical pathways have been identified, the underlying mechanical basis including the forces…
The optimal design seeks the best possible solution(s) for a mechanical structure, device, or system, satisfying a series of requirements and leading to the best performance. In this work, optimized nozzle shapes have been designed for a…
This paper presents in-situ X-ray computed tomography (CT) experiments used to study the flow behavior within a conventional hot-end of a fused filament fabrication printer. Three types of experiments were performed to better understand the…
Biological cells can actively tune their intracellular architecture according to their overall shape. Here we explore the rheological implication of such coupling in a minimal model of a dense cellular material where each cell exerts an…
A thorough study of shear stress within the lattice Boltzmann method is provided. Via standard multiscale Chapman-Enskog expansion we investigate the dependence of the error in shear stress on grid resolution showing that the shear stress…
Recent experiments in various cell types have shown that two-dimensional tissues often display local nematic order, with evidence of extensile stresses manifest in the dynamics of topological defects. Using a mesoscopic model where tissue…
This paper describes a lattice Boltzmann-based binary fluid model for inkjet printing. In this model, a time-dependent driving force is applied to actuate the droplet ejection. As a result, the actuation can be accurately controlled by…
The flow driven by a rapidly expanding and collapsing cavitation bubble in a narrow cylindrical gap is studied with the volume of fluid method. The simulations reveal a developing plug flow during the early expansion followed by flow…
We measure the dynamical mechanical properties of human red blood cells. Single cell response is measured with optical tweezers. We investigate both the stress relaxation following a fast deformation, and the effect of varying the strain…
The alignment of fibers and cells in living tissues affect their mechanical properties and functionality. In this context, one can draw an analogy between tissues and nematic liquid crystal elastomers. We explore this analogy by growing…
A hallmark of biological tissues, viewed as complex cellular materials, is the active generation of mechanical stresses by cellular processes, such as cell divisions. Each cellular event generates a force dipole that deforms the surrounding…
Understanding how cells escape from embedded spheroids requires a mechanical framework linking stress generation within cells, across cells, and between cells and the surrounding extracellular matrix (ECM). We develop such a framework by…
We formulate the continuum field equations and constitutive equations that govern deformation, stress, and electric current flow in a Li-ion half-cell. The model considers mass transport through the system, deformation and stress in the…
Cell extrusion is an essential mechanism for controlling cell density in epithelial tissues. Another essential element of epithelia is curvature, which is required to achieve complex shapes, like in the lung or intestine. Here we introduce…
During embryonic morphogenesis, tissues undergo dramatic deformations in order to form functional organs. Similarly, in adult animals, living cells and tissues are continually subjected to forces and deformations. Therefore, the success of…
Tissues of living cells are a prime example of active fluids. There is experimental evidence that tissues generate extensile active stress even though their constituting cells are contractile. Fluctuating forces that could result from…