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Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy…

Computer Vision and Pattern Recognition · Computer Science 2019-05-21 Adrian Shajkofci , Michael Liebling

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet

Ultrasound imaging systems rely on accurate point spread function (PSF) estimation to support advanced image quality enhancement techniques such as deconvolution and speckle reduction. Phase aberration, caused by sound speed inhomogeneity…

Signal Processing · Electrical Eng. & Systems 2025-02-24 Wei-Hsiang Shen , Yu-An Lin , Meng-Lin Li

Fluorescence microscopy plays an important role in biomedical research. The depth-variant point spread function (PSF) of a fluorescence microscope produces low-quality images especially in the out-of-focus regions of thick specimens.…

Image and Video Processing · Electrical Eng. & Systems 2019-07-09 Da He , De Cai , Jiasheng Zhou , Jiajia Luo , Sung-Liang Chen

In this paper, we propose a novel design of image deblurring in the form of one-shot convolution filtering that can directly convolve with naturally blurred images for restoration. The problem of optical blurring is a common disadvantage to…

Image and Video Processing · Electrical Eng. & Systems 2019-07-22 Mahdi S. Hosseini , Konstantinos N. Plataniotis

Deconvolution is the most commonly used image processing method to remove the blur caused by the point-spread-function (PSF) in optical imaging systems. While this method has been successful in deblurring, it suffers from several…

Image and Video Processing · Electrical Eng. & Systems 2019-10-10 Huangxuan Zhao , Ziwen Ke , Ningbo Chen , Ke Li , Lidai Wang , Xiaojing Gong , Wei Zheng , Liang Song , Zhicheng Liu , Dong Liang , Chengbo Liu

Optical imaging quality can be severely degraded by system and sample induced aberrations. Existing adaptive optics systems typically rely on iterative search algorithm to correct for aberrations and improve images. This study demonstrates…

The process of acquiring microscopic images in life sciences often results in image degradation and corruption, characterised by the presence of noise and blur, which poses significant challenges in accurately analysing and interpreting the…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Tomáš Chobola , Gesine Müller , Veit Dausmann , Anton Theileis , Jan Taucher , Jan Huisken , Tingying Peng

Richardson-Lucy deconvolution is widely used to restore images from degradation caused by the broadening effects of a point spread function and corruption by photon shot noise, in order to recover an underlying object. In practice, this is…

Computer Vision and Pattern Recognition · Computer Science 2024-11-05 Zachary H. Hendrix , Peter T. Brown , Tim Flanagan , Douglas P. Shepherd , Ayush Saurabh , Steve Pressé

Microscopy is one of the most essential imaging techniques in life sciences. High-quality images are required in order to solve (potentially life-saving) biomedical research problems. Many microscopy techniques do not achieve sufficient…

Computer Vision and Pattern Recognition · Computer Science 2018-10-24 Joris Roels , Jan Aelterman , Jonas De Vylder , Hiep Luong , Yvan Saeys , Wilfried Philips

Phase aberration is an inherent side effect of ultrasound imaging due to the speed of sound inhomogeneity nature of human tissues, resulting in focusing error and reduced image contrast. This work introduces a phase aberration correction…

Signal Processing · Electrical Eng. & Systems 2023-08-29 Wei-Hsiang Shen , Yu-An Lin , Pai-Chi Li , Meng-Lin Li

Estimation of optical aberrations from volumetric intensity images is a key step in sensorless adaptive optics for 3D microscopy. Recent approaches based on deep learning promise accurate results at fast processing speeds. However,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Debayan Saha , Uwe Schmidt , Qinrong Zhang , Aurelien Barbotin , Qi Hu , Na Ji , Martin J. Booth , Martin Weigert , Eugene W. Myers

Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data. While deconvolution methods and other…

Computer Vision and Pattern Recognition · Computer Science 2017-04-06 Martin Weigert , Loic Royer , Florian Jug , Gene Myers

Due to limited size and imperfect of the optical components in a spectrometer, aberration has inevitably been brought into two-dimensional multi-fiber spectrum image in LAMOST, which leads to obvious spacial variation of the point spread…

Instrumentation and Methods for Astrophysics · Physics 2020-10-28 Jiali Xu , Qian Yin , Ping Guo , Xin Zheng

Image deconvolution is still to be a challenging ill-posed problem for recovering a clear image from a given blurry image, when the point spread function is known. Although competitive deconvolution methods are numerically impressive and…

Computer Vision and Pattern Recognition · Computer Science 2016-09-07 Hang Yang , Zhongbo Zhang , Yujing Guan

A point-spread function describes the optics of an imaging system and can be used to correct collected images for instrumental effects. The state of the art for deconvolving images with the point-spread function is the Richardson-Lucy…

Instrumentation and Methods for Astrophysics · Physics 2024-05-07 Stefan Johann Hofmeister

The optics of any camera degrades the sharpness of photographs, which is a key visual quality criterion. This degradation is characterized by the point-spread function (PSF), which depends on the wavelengths of light and is variable across…

Image and Video Processing · Electrical Eng. & Systems 2022-08-02 Thomas Eboli , Jean-Michel Morel , Gabriele Facciolo

This paper demonstrates a practical method that can correct spatial varying blur from a set of images of the same object. The algorithm jointly estimates the object and local point spread functions~(PSF). The method prioritizes sections…

Image and Video Processing · Electrical Eng. & Systems 2020-11-04 Wouter van de Ketterij , Oleg Soloviev , Michel Verhaegen

In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…

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