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Related papers: SOFFLFM: Super-resolution optical fluctuation Four…

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This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…

Optics · Physics 2015-10-30 Roarke Horstmeyer , Changhuei Yang

Fourier ptychography microscopy (FPM) is a new computational imaging technique that can provide gigapixel images with both high resolution and a wide field of view (FOV). However, time consuming of the data-acquisition process is a critical…

Image and Video Processing · Electrical Eng. & Systems 2018-08-15 Ao Zhou , Ni Chen , Haichao Wang , Guohai Situ

We present a new two-snapshot structured light illumination (SLI) reconstruction algorithm for fast image acquisition. The new algorithm, which only requires two mutually {\pi} phase-shifted raw structured images, is implemented on a…

Optics · Physics 2017-10-25 Yunlong Meng , Wei Lin , Chenglin Li , Shih-chi Chen

We provide a quantitative analysis of super-resolution imaging techniques which exploit temporal fluctuations of luminosity of the sources in order to beat the Rayleigh limit. We define an operationally justified resolution gain figure of…

Quantum Physics · Physics 2021-06-08 Stanislaw Kurdzialek , Rafal Demkowicz-Dobrzanski

Optical phase-space functions describe spatial and angular information simultaneously; examples include light fields in ray optics and Wigner functions in wave optics. Measurement of phase-space enables digital refocusing, aberration…

Optics · Physics 2017-08-02 Hsiou-Yuan Liu , Jingshan Zhong , Laura Waller

Optical diffraction tomography (ODT) is a three-dimensional (3D) label-free imaging technique. The 3D refractive index distribution of a sample can be reconstructed from multiple two-dimensional optical field images via ODT. Herein, we…

Optics · Physics 2021-02-03 Chansuk Park , Kyeoreh Lee , Yoonseok Baek , Yongkeun Park

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Varun Mannam , Xiaotong Yuan , Scott Howard

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Ultrafast 3D imaging is indispensable for visualizing complex and dynamic biological processes. Conventional scanning-based techniques necessitate an inherent trade-off between acquisition speed and space-bandwidth product (SBP). Emerging…

Fourier ptychographic microscopy (FPM), characterized by high-throughput computational imaging, theoretically provides a cunning solution to the trade-off between spatial resolution and field of view (FOV), which has a promising prospect in…

Optics · Physics 2024-02-29 Shuhe Zhang , Aiye Wang , Jinghao Xu , Tianci Feng , Jinhua Zhou , An Pan

Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

Ultrasound super-localization microscopy techniques presented in the last few years enable non-invasive imaging of vascular structures at the capillary level by tracking the flow of ultrasound contrast agents (gas microbubbles). However,…

Tissues and Organs · Quantitative Biology 2016-08-10 Avinoam Bar-Zion , Charles Tremblay-Darveau , Oren Solomon , Dan Adam , Yonina C. Eldar

Imaging below the diffraction limit is always a public interest because of the restricted resolution of conventional imaging systems. To beat the limit, evanescent harmonics decaying in space must participate in the imaging process. Here,…

Optics · Physics 2019-10-02 Tie-Jun Huang , Li-Zheng Yin , Ya Shuang , Jiang-Yu Liu , Yunhua Tan , Pu-Kun Liu

Light-field microscopy (LFM) enables rapid volumetric imaging through single-frame acquisition and fast 3D reconstruction algorithms. The high speed and low phototoxicity of LFM make it highly suitable for real-time 3D fluorescence imaging,…

Optics · Physics 2025-02-24 Bohan Qu , Zhouyu Jin , You Zhou , Bo Xiong , Xun Cao

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Accurate calibration of internal parameters is a crucial yet challenging prerequisite for 3D reconstruction using light field cameras. In this paper, we propose a linear fractional transformation(LFT) parameter $\alpha$ to decoupled the…

Computer Vision and Pattern Recognition · Computer Science 2025-11-07 Zhong Chen , Changfeng Chen

Fourier ptychographi cmicroscopy(FPM) is a newly developed computational imaging technique that can provide gigapixel images with both high resolution (HR) and wide field of view (FOV). However, the positional misalignment of the LED array…

Computer Vision and Pattern Recognition · Computer Science 2018-09-03 Ao Zhou , Wei Wang , Ni Chen , Edmund Y. Lam , Byoungho Lee , Guohai Situ

Real-time 3D fluorescence microscopy is crucial for the spatiotemporal analysis of live organisms, such as neural activity monitoring. The eXtended field-of-view light field microscope (XLFM), also known as Fourier light field microscope,…

Image and Video Processing · Electrical Eng. & Systems 2023-06-16 Josué Page Vizcaíno , Panagiotis Symvoulidis , Zeguan Wang , Jonas Jelten , Paolo Favaro , Edward S. Boyden , Tobias Lasser

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang