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Correlative light-electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Most CLEM techniques use…

Transmission electron microscopes (TEMs) enable atomic-scale imaging and characterisation, driving advances across fields from materials science to biology. Quantum correlations, specifically entanglement, may provide a basis for novel…

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

State-of-the-art electron microscopes such as scanning electron microscopes (SEM), scanning transmission electron microscopes (STEM) and transmission electron microscopes (TEM) have become increasingly sophisticated. However, the quality of…

Computational Physics · Physics 2023-03-31 I. Lobato , T. Friedrich , S. Van Aert

If we are ever to unravel the mysteries of brain function at its most fundamental level, we will need a precise understanding of how its component neurons connect to each other. Electron Microscopes (EM) can now provide the nanometer…

Computer Vision and Pattern Recognition · Computer Science 2016-09-28 Pascal Fua , Graham Knott

Defects in crystalline materials control the properties of materials, and their characterization focuses our strategies to optimize performance. Electron microscopy has served as the backbone of our understanding of defect structure and…

Materials Science · Physics 2019-03-19 Daniel S. Gianola , T. Ben Britton , Stefan Zaefferer

In multi-view fluorescence microscopy, each angular acquisition needs to be aligned with care to obtain an optimal volumetric reconstruction. Here, instead, we propose a neat protocol based on auto-correlation inversion, that leads directly…

Image and Video Processing · Electrical Eng. & Systems 2021-10-22 Daniele Ancora , Gianluca Valentini , Antonio Pifferi , Andrea Bassi

Low-energy electron microscopy (LEEM) is a surface science method that works primarily in the UHV environment. It provides information complementary to the other established techniques: it extends the limited view of scanning probe…

Mesoscale and Nanoscale Physics · Physics 2025-07-16 Jan Čechal , Pavel Procházka

Correlative light electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe.…

Self-supervised monocular depth estimation serves as a key task in the development of endoscopic navigation systems. However, performance degradation persists due to uneven illumination inherent in endoscopic images, particularly in…

Computer Vision and Pattern Recognition · Computer Science 2025-11-26 Mingyang Ou , Haojin Li , Yifeng Zhang , Ke Niu , Zhongxi Qiu , Heng Li , Jiang Liu

Scanning Electron Microscopy (SEM) is indispensable in modern materials science, enabling high-resolution imaging across a wide range of structural, chemical, and functional investigations. However, SEM imaging remains constrained by…

Cellular Electron Cryo-Tomography (CECT) is a powerful imaging technique for the 3D visualization of cellular structure and organization at submolecular resolution. It enables analyzing the native structures of macromolecular complexes and…

Quantitative Methods · Quantitative Biology 2018-05-16 Chang Liu , Xiangrui Zeng , Ruogu Lin , Xiaodan Liang , Zachary Freyberg , Eric Xing , Min Xu

Blind deconvolution aims to recover an original image from a blurred version in the case where the blurring kernel is unknown. It has wide applications in diverse fields such as astronomy, microscopy, and medical imaging. Blind…

Numerical Analysis · Mathematics 2024-02-06 Markus Haltmeier , Gyeongha Hwang

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

The living body is composed of innumerable fine and complex structures and although these structures have been studied in the past, a vast amount of information pertaining to them still remains unknown. When attempting to observe these…

Medical Physics · Physics 2021-11-30 Raku Son , Kenji Yamazawa , Akiko Oguchi , Mitsuo Suga , Masaru Tamura , Yasuhiro Murakawa , Satoshi Kume

The most established method of reconstructing neural circuits from animals involves slicing tissue very thin, then taking mosaics of electron microscope (EM) images. To trace neurons across different images and through different sections,…

Quantitative Methods · Quantitative Biology 2013-04-23 Louis K. Scheffer , Bill Karsh , Shiv Vitaladevun

Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…

Optics · Physics 2020-11-04 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

This paper studies 3D low-dose computed tomography (CT) imaging. Although various deep learning methods were developed in this context, typically they focus on 2D images and perform denoising due to low-dose and deblurring for…

Image and Video Processing · Electrical Eng. & Systems 2024-01-10 Zhihao Chen , Chuang Niu , Qi Gao , Ge Wang , Hongming Shan

Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images,…

Recently, deep learning approaches have become the main research frontier for biological image reconstruction and enhancement problems thanks to their high performance, along with their ultra-fast inference times. However, due to the…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Mehmet Akçakaya , Burhaneddin Yaman , Hyungjin Chung , Jong Chul Ye
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