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We explore the possibility of applying physical phase plates (PPs) in combination with aberration-corrected transmission electron microscopy. Phase-contrast transfer characteristics are calculated and compared for a thin-film based Zernike…

Instrumentation and Detectors · Physics 2022-05-10 Simon Hettler , Raul Arenal

In this theoretical study we analyze contrast transfer of weak-phase objects in a transmission electron microscope, which is equipped with an aberration corrector (Cs-corrector) in the imaging lens system and a physical phase plate in the…

Materials Science · Physics 2010-09-22 B. Gamm , K. Schultheiß , R. R. Schröder , D. Gerthsen

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a…

Quantitative Methods · Quantitative Biology 2024-03-14 Jeremy J. Axelrod , Jessie T. Zhang , Petar N. Petrov , Robert M. Glaeser , Holger Mueller

Low image contrast is a major limitation in transmission electron microscopy, since samples with low atomic number only weakly phase-modulate the illuminating electron beam, and beam-induced sample damage limits the usable electron dose.…

Instrumentation and Detectors · Physics 2024-03-19 Jeremy J. Axelrod

We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser-electron-interaction,…

Instrumentation and Detectors · Physics 2010-09-10 Holger Mueller , Jian Jin , Radostin Danev , John Spence , Howard Padmore , Robert M. Glaeser

For decades since the development of phase-contrast optical microscopy, an analogous approach has been sought for maximizing the image contrast of weakly-scattering objects in transmission electron microscopy (TEM). The recent development…

Numerous physical phase plates (PP) for phase-contrast enhancement in transmission electron microscopy (TEM) have been proposed and studied with the hole-free or Volta PP having a high impact and interest in recent years. This study is…

Instrumentation and Detectors · Physics 2021-05-25 Simon Hettler , Raul Arenal

The use of phase plates in the back focal plane of a microscope is a well established technique in optical microscopy to increase the contrast of weakly interacting samples and is gaining interest in electron microscopy as well. In this…

Optics · Physics 2016-08-31 Roeland Juchtmans , Laura Clark , Axel Lubk , Jo Verbeeck

A novel scheme for Zernike-type hard X-ray phase-contrast imaging is proposed. The scheme relies on X-ray dynamical diffraction in a two-block crystal system with parallel crystal plates of equal thickness. A phase shifter providing a…

Optics · Physics 2026-02-27 Levon Haroutunyan

Electron microscopy is a powerful tool for visualizing the shapes of sub-nanometer objects. However, contrast is not in proportional to density distribution, and therefore achieving a quantitative understanding of specimens is not…

Instrumentation and Detectors · Physics 2020-05-14 Masato Tomita , Yukinori Nagatani , Kazuyoshi Murata , Atsushi Momose

Nonlinear metasurfaces that dynamically manipulate the phase of a passing light beam are of interest for a wide range of applications. The controlled operation of such devices requires accurate measurements of the optical transmission phase…

Scanning transmission electron microscopy (STEM) provides high-resolution visualization of atomic structures as well as various functional imaging modes utilizing phase contrast. In this study we introduce a semicircular aperture in STEM…

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Phase-contrast imaging is an emerging technology that may increase the signal-difference-to-noise ratio in medical imaging. One of the most promising phase-contrast techniques is Talbot interferometry, which, combined with energy-sensitive…

A device based on a three-block Fresnel zone plate interferometer is proposed for hard X-ray phase-contrast imaging. The device combines a low requirement for the coherence of the initial radiation (the interferometer operates in the…

Instrumentation and Detectors · Physics 2021-04-22 L. A. Haroutunyan

In order to efficiently image a non-absorbing sample (a phase object), dedicated phase contrast optics are required. Typically, these optics are designed with the assumption that the sample is weakly scattering, implying a linear relation…

Optics · Physics 2020-10-22 Stewart Koppell , Mark Kasevich

We discuss the use of parametric phase-diverse phase retrieval as an in-situ high-fidelity wavefront measurement method to characterize and optimize the transmitted wavefront of a high-contrast coronagraphic instrument. We apply our method…

Instrumentation and Methods for Astrophysics · Physics 2019-03-06 Gregory R. Brady , Christopher Moriarty , Peter Petrone , Iva Laginja , Keira Brooks , Tom Comeau , Lucie Leboulleux , Remi Soummer

A transmission electron microscope that takes advantage of superconducting quantum circuitry is proposed. The microscope is designed to improve image contrast of radiation-sensitive weak phase objects, in particular biological specimens.…

Quantum Physics · Physics 2015-05-27 Hiroshi Okamoto

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of…

Future Extremely Large Telescopes will adopt segmented primary mirrors with several hundreds of segments. Cophasing of the segments together is essential to reach high wavefront quality. The phasing sensor must be able to maintain very high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-27 Arthur Vigan , Kjetil Dohlen , Silvio Mazzanti
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