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An holographic microscopy reconstruction method compatible with high numerical aperture microscope objective (MO) up to NA=1.4 is proposed. After off axis and reference field curvature corrections, and after selection of the +1 grating…

Optics · Physics 2015-11-16 Nicolas Verrier , Dario Donnarumma , Gilles Tessier , Michel Gross

High-throughput biological imaging is often constrained by a trade-off between acquisition speed and image quality. Fast imaging modalities, such as wide-field fluorescence microscopy, enable large-scale data acquisition but suffer from…

Image and Video Processing · Electrical Eng. & Systems 2026-04-20 Dominik Panek , Carina Rząca , Maksymilian Szczypior , Joanna Sorysz , Krzysztof Misztal , Zbigniew Baster , Zenon Rajfur

Dusty star-forming galaxies (DSFGs) dominate the far-infrared and sub-millimetre number counts, but single-dish surveys suffer from poor angular resolution, complicating mult-wavelength counterpart identification. Prior-driven deblending…

The resolution limits of classical spectroscopy can be surpassed by quantum-inspired methods leveraging the information contained in the phase of the complex electromagnetic field. Their counterpart in spatial imaging has been widely…

Optics · Physics 2024-09-02 Michał Lipka , Michał Parniak

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

We report on a new x-ray imaging method, which combines the high spatial resolution of coherent diffraction imaging with the ability of dark field microscopy to map grains within thick polycrystalline specimens. An x-ray objective serves to…

Instrumentation and Detectors · Physics 2020-07-15 Anders Filsøe Pedersen , Virginie Chamard , Carsten Detlefs , Tao Zhou , Dina Carbone , Henning Friis Poulsen

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

High contrast imaging (HCI) systems rely on active wavefront control (WFC) to deliver deep raw contrast in the focal plane, and on calibration techniques to further enhance contrast by identifying planet light within the residual speckle…

We demonstrate a simple scheme for high-resolution imaging of nanoplasmonic structures that basically removes most of the resolution limiting allowed light usually transmitted to the far field. This is achieved by implementing a Fourier…

Optics · Physics 2015-06-18 O. Mollet , S. Huant , A. Drezet

Light scattering by tissue severely limits how deep beneath the surface one can image, and the spatial resolution one can obtain from these images. Diffuse optical tomography (DOT) is one of the most powerful techniques for imaging deep…

Image and Video Processing · Electrical Eng. & Systems 2021-05-31 Yongyi Zhao , Ankit Raghuram , Hyun K. Kim , Andreas H. Hielscher , Jacob T. Robinson , Ashok Veeraraghavan

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

Scanning electron microscopy (SEM) is indispensable in diverse applications ranging from microelectronics to food processing because it provides large depth-of-field images with a resolution beyond the optical diffraction limit. However,…

Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining…

Learning-based image super-resolution aims to reconstruct high-frequency (HF) details from the prior model trained by a set of high- and low-resolution image patches. In this paper, HF to be estimated is considered as a combination of two…

Computer Vision and Pattern Recognition · Computer Science 2016-11-18 Jian Zhang , Chen Zhao , Ruiqin Xiong , Siwei Ma , Debin Zhao

Following the recent developement of Fourier ptychographic microscopy (FPM) in the visible range by Zheng et al. (2013), we propose an adaptation for hard x-rays. FPM employs ptychographic reconstruction to merge a series of low-resolution,…

Instrumentation and Detectors · Physics 2016-09-27 H. Simons , H. F. Poulsen , J. P. Guigay , C. Detlefs

Femtosecond laser ablation has been demonstrated to be a versatile tool to produce micro/nanoscale features with high precision and accuracy. However, the use of high laser fluence to increase the ablation efficiency usually results in…

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

We present a progress report on the design and construction of the Field-Imaging Far-Infrared Line Spectrometer (FIFI LS) for the SOFIA airborne observatory. The design of the instrument is driven by the goal of maximizing observing…

Astrophysics · Physics 2015-06-24 L. W. Looney , N. Geis , R. Genzel , W. K. Park , A. Poglitsch , W. Raab , D. Rosenthal , A. Urban , T. Henning