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Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

Data Analysis, Statistics and Probability · Physics 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

As a neuromorphic sensor with high temporal resolution, spike camera can generate continuous binary spike streams to capture per-pixel light intensity. We can use reconstruction methods to restore scene details in high-speed scenarios.…

Computer Vision and Pattern Recognition · Computer Science 2024-07-09 Liwen Hu , Ziluo Ding , Mianzhi Liu , Lei Ma , Tiejun Huang

The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

Data Analysis, Statistics and Probability · Physics 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

This work proposes a deep learning (DL)-based framework, namely Sim2Real, for spectral signal reconstruction in reconstructive spectroscopy, focusing on efficient data sampling and fast inference time. The work focuses on the challenge of…

Machine Learning · Computer Science 2024-08-20 Jiyi Chen , Pengyu Li , Yutong Wang , Pei-Cheng Ku , Qing Qu

Spike cameras, as innovative neuromorphic devices, generate continuous spike streams to capture high-speed scenes with lower bandwidth and higher dynamic range than traditional RGB cameras. However, reconstructing high-quality images from…

Computer Vision and Pattern Recognition · Computer Science 2025-03-05 Kang Chen , Yajing Zheng , Tiejun Huang , Zhaofei Yu

A method is proposed for assessing the temporal resolution of Structured Illumination Microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating…

Optics · Physics 2020-08-05 Abderrahim Boualam , Christopher J Rowlands

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong

In recent years there has been great interest in using deep neural networks (DNN) for super-resolution image reconstruction including for structured illumination microscopy (SIM). While these methods have shown very promising results, they…

Image and Video Processing · Electrical Eng. & Systems 2023-03-22 Zachary Burns , Zhaowei Liu

Spike camera is a new type of bio-inspired vision sensor that records light intensity in the form of a spike array with high temporal resolution (20,000 Hz). This new paradigm of vision sensor offers significant advantages for many vision…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Lin Zhu , Yunlong Zheng , Mengyue Geng , Lizhi Wang , Hua Huang

Photoluminesce (PL) spectroscopy offers excellent methods for mapping the PL decay on the nanosecond time scale. However, capturing maps of emission dynamics on the microsecond time scale can be highly time-consuming. We present a new…

Optics · Physics 2021-02-24 Jiri Junek , Karel Zidek

Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…

Image and Video Processing · Electrical Eng. & Systems 2021-07-14 Qinnan Zhang , En Bo , Jiawei Chen , Jiaosheng Li , Heming Jiang , Xiaoxu Lu , Liyun Zhong , Jindong Tian

We propose SLARM, a feed-forward model that unifies dynamic scene reconstruction, semantic understanding, and real-time streaming inference. SLARM captures complex, non-uniform motion through higher-order motion modeling, trained solely on…

Computer Vision and Pattern Recognition · Computer Science 2026-03-27 Zhicheng Qiu , Jiarui Meng , Tong-an Luo , Yican Huang , Xuan Feng , Xuanfu Li , ZHan Xu

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

Structure from motion (SfM) enables us to reconstruct a scene via casual capture from cameras at different viewpoints, and novel view synthesis (NVS) allows us to render a captured scene from a new viewpoint. Both are hard with casual…

Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM…

Neuromorphic vision sensors, such as the dynamic vision sensor (DVS) and spike camera, have gained increasing attention in recent years. The spike camera can detect fine textures by mimicking the fovea in the human visual system, and output…

Computer Vision and Pattern Recognition · Computer Science 2024-12-17 Wei Zhang , Weiquan Yan , Yun Zhao , Wenxiang Cheng , Gang Chen , Huihui Zhou , Yonghong Tian