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Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

Optics · Physics 2022-03-09 James D. Manton

Structured illumination microscopy (SIM) achieves superresolution in fluorescence imaging through patterned illumination and computational image reconstruction, yet current methods require bulky, costly modulation optics and high-precision…

Optics · Physics 2024-04-15 Tamal Roy , Peter T. Brown , Douglas P. Shepherd , Lisa V. Poulikakos

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

Computer Vision and Pattern Recognition · Computer Science 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

Computer Vision and Pattern Recognition · Computer Science 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

Image and Video Processing · Electrical Eng. & Systems 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

Optics · Physics 2024-11-18 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…

Image and Video Processing · Electrical Eng. & Systems 2021-07-14 Qinnan Zhang , En Bo , Jiawei Chen , Jiaosheng Li , Heming Jiang , Xiaoxu Lu , Liyun Zhong , Jindong Tian

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Over the past decade, structured illumination microscopy (SIM) has found its niche in super-resolution (SR) microscopy due to its fast imaging speed and low excitation intensity. However, due to the significantly higher light dose compared…

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