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Composite DNA is a recent method to increase the base alphabet size in DNA-based data storage.This paper models synthesizing and sequencing of composite DNA and introduces coding techniques to correct substitutions, losses of entire…

Information Theory · Computer Science 2025-10-29 Frederik Walter , Omer Sabary , Antonia Wachter-Zeh , Eitan Yaakobi

This paper concerns non-overlapping codes, block codes motivated by synchronisation and DNA-based storage applications. Most existing constructions of these codes do not account for the restrictions posed by the physical properties of…

Information Theory · Computer Science 2025-02-05 Lidija Stanovnik , Miha Moškon , Miha Mraz

In this work, we investigate a challenging problem, which has been considered to be an important criterion in designing codewords for DNA computing purposes, namely secondary structure avoidance in single-stranded DNA molecules. In short,…

Information Theory · Computer Science 2023-02-28 Tuan Thanh Nguyen , Kui Cai , Han Mao Kiah , Duc Tu Dao , Kees A. Schouhamer Immink

Motivated by DNA storage in living organisms, and by known biological mutation processes, we study the reverse-complement string-duplication system. We fully classify the conditions under which the system has full expressiveness, for all…

Information Theory · Computer Science 2021-12-23 Eyar Ben-Tolila , Moshe Schwartz

Most DNA sequencing technologies are based on the shotgun paradigm: many short reads are obtained from random unknown locations in the DNA sequence. A fundamental question, studied in arXiv:1203.6233, is what read length and coverage depth…

Information Theory · Computer Science 2022-02-09 Aditya Narayan Ravi , Alireza Vahid , Ilan Shomorony

DNA strings and their properties are widely studied since last 20 years due to its applications in DNA computing. In this area, one designs a set of DNA strings (called DNA code) which satisfies certain thermodynamic and combinatorial…

Information Theory · Computer Science 2025-10-03 Krishna Gopal Benerjee , Manish K Gupta

Reconstruction codes are generalizations of error-correcting codes that can correct errors by a given number of noisy reads. The study of such codes was initiated by Levenshtein in 2001 and developed recently due to applications in modern…

Information Theory · Computer Science 2023-02-21 Zuo Ye , Xin Liu , Xiande Zhang , Gennian Ge

At the core of high throughput DNA sequencing platforms lies a bio-physical surface process that results in a random geometry of clusters of homogenous short DNA fragments typically hundreds of base pairs long - bridge amplification. The…

Genomics · Quantitative Biology 2015-08-13 Eliza O'Reilly , Francois Baccelli , Gustavo de Veciana , Haris Vikalo

The prevalent technique for DNA sequencing consists of two main steps: shotgun sequencing, where many randomly located fragments, called reads, are extracted from the overall sequence, followed by an assembly algorithm that aims to…

Genomics · Quantitative Biology 2016-01-28 Shirshendu Ganguly , Elchanan Mossel , Miklos Z. Racz

The problem of reconstructing a sequence from the set of its length-$k$ substrings has received considerable attention due to its various applications in genomics. We study an uncoded version of this problem where multiple random sources…

Information Theory · Computer Science 2023-05-11 Kel Levick , Ilan Shomorony

The process of DNA-based data storage (DNA storage for short) can be mathematically modelled as a communication channel, termed DNA storage channel, whose inputs and outputs are sets of unordered sequences. To design error correcting codes…

Information Theory · Computer Science 2020-06-11 Wentu Song , Kui Cai , Kees A. Schouhamer Immink

The high-throughput short-reads RNA-seq protocols often produce paired-end reads, with the middle portion of the fragments being unsequenced. We explore if the full-length fragments can be computationally reconstructed from the sequenced…

Genomics · Quantitative Biology 2023-10-06 Xiang Li , Mingfu Shao

Relative compression, where a set of similar strings are compressed with respect to a reference string, is a very effective method of compressing DNA datasets containing multiple similar sequences. Relative compression is fast to perform…

Quantitative Methods · Quantitative Biology 2011-06-21 Shanika Kuruppu , Simon Puglisi , Justin Zobel

A method to construct and count all the linear codes (of arbitrary length) in $\mathbb{F}_{4}$ that are invariant under reverse permutation and that contain the repetition code is presented. These codes are suitable for constructing DNA…

Information Theory · Computer Science 2025-06-25 E. J. García-Claro

We introduce a novel method to analyse complete genomes and recognise some distinctive features by means of an adaptive compression algorithm, which is not DNA-oriented. We study the Information Content as a function of the number of…

Genomics · Quantitative Biology 2007-05-23 Giulia Menconi

As DNA data storage moves closer to practical deployment, minimizing sequencing coverage depth is essential to reduce both operational costs and retrieval latency. This paper addresses the recently studied Random Access Problem, which…

Information Theory · Computer Science 2026-01-13 Chen Wang , Eitan Yaakobi

DNA synthesis is considered as one of the most expensive components in current DNA storage systems. In this paper, focusing on a common synthesis machine, which generates multiple DNA strands in parallel following a fixed supersequence,we…

Information Theory · Computer Science 2025-05-13 Yajuan Liu , Tolga M. Duman

We describe properties and constructions of constraint-based codes for DNA-based data storage which account for the maximum repetition length and AT/GC balance. We present algorithms for computing the number of sequences with maximum…

Information Theory · Computer Science 2018-12-18 Kees A. Schouhamer Immink , Kui Cai

We consider the problem of assembling a sequence based on a collection of its substrings observed through a noisy channel. The mathematical basis of the problem is the construction and design of sequences that may be discriminated based on…

Information Theory · Computer Science 2015-11-04 Han Mao Kiah , Gregory J. Puleo , Olgica Milenkovic

We provide an overview of current approaches to DNA-based storage system design and accompanying synthesis, sequencing and editing methods. We also introduce and analyze a suite of new constrained coding schemes for both archival and random…

Emerging Technologies · Computer Science 2015-07-08 S. M. Hossein Tabatabaei Yazdi , Han Mao Kiah , Eva Ruiz Garcia , Jian Ma , Huimin Zhao , Olgica Milenkovic